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• W4240790008 abstract "Abstract The coronavirus disease 2019 (COVID-19) pandemic, caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is an ongoing global health threat with more than two million infected people since its emergence in late 2019. Detailed knowledge of the molecular biology of the infection is indispensable for understanding of the viral replication, host responses, and disease progression. We provide gene expression profiles of SARS-CoV and SARS-CoV-2 infections in three human cell lines (H1299, Caco-2 and Calu-3 cells), using bulk and single-cell transcriptomics. Small RNA profiling showed strong expression of the immunity and inflammation-associated microRNA miRNA-155 upon infection with both viruses. SARS-CoV-2 elicited approximately two-fold higher stimulation of the interferon response compared to SARS-CoV in the permissive human epithelial cell line Calu-3, and induction of cytokines such as CXCL10 or IL6. Single cell RNA sequencing data showed that canonical interferon stimulated genes such as IFIT2 or OAS2 were broadly induced, whereas interferon beta (IFNB1) and lambda (IFNL1-4) were expressed only in a subset of infected cells. In addition, temporal resolution of transcriptional responses suggested interferon regulatory factors (IRFs) activities precede that of nuclear factor-κB (NF-κB). Lastly, we identified heat shock protein 90 (HSP90) as a protein relevant for the infection. Inhibition of the HSP90 charperone activity by Tanespimycin/17-N-allylamino-17-demethoxygeldanamycin (17-AAG) resulted in a reduction of viral replication, and of TNF and IL1B mRNA levels. In summary, our study established in vitro cell culture models to study SARS-CoV-2 infection and identified HSP90 protein as potential drug target for therapeutic intervention of SARS-CoV-2 infection." @default.
• W4240790008 created "2022-05-12" @default.
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• W4240790008 date "2020-05-05" @default.
• W4240790008 modified "2023-10-05" @default.
• W4240790008 title "Bulk and single-cell gene expression profiling of SARS-CoV-2 infected human cell lines identifies molecular targets for therapeutic intervention" @default.
• W4240790008 cites W144423133 @default.
• W4240790008 cites W1544561280 @default.
• W4240790008 cites W1545869027 @default.
• W4240790008 cites W1546689286 @default.
• W4240790008 cites W1573098634 @default.
• W4240790008 cites W1862727846 @default.
• W4240790008 cites W1964833020 @default.
• W4240790008 cites W1965158331 @default.
• W4240790008 cites W1969841753 @default.
• W4240790008 cites W1975915247 @default.
• W4240790008 cites W1989207797 @default.
• W4240790008 cites W1989600727 @default.
• W4240790008 cites W1994195919 @default.
• W4240790008 cites W2001029058 @default.
• W4240790008 cites W2001788362 @default.
• W4240790008 cites W2029955900 @default.
• W4240790008 cites W2035618305 @default.
• W4240790008 cites W2037692580 @default.
• W4240790008 cites W2039689649 @default.
• W4240790008 cites W2044973755 @default.
• W4240790008 cites W2053476250 @default.
• W4240790008 cites W2055463868 @default.
• W4240790008 cites W2058008662 @default.
• W4240790008 cites W2059763393 @default.
• W4240790008 cites W2068633454 @default.
• W4240790008 cites W2072517432 @default.
• W4240790008 cites W2079294041 @default.
• W4240790008 cites W2079674166 @default.
• W4240790008 cites W2088138646 @default.
• W4240790008 cites W2094331408 @default.
• W4240790008 cites W2102619694 @default.
• W4240790008 cites W2103208261 @default.
• W4240790008 cites W2110417468 @default.
• W4240790008 cites W2120604940 @default.
• W4240790008 cites W2129467097 @default.
• W4240790008 cites W2130116522 @default.
• W4240790008 cites W2130305733 @default.
• W4240790008 cites W2130641893 @default.
• W4240790008 cites W2132702191 @default.
• W4240790008 cites W2133402546 @default.
• W4240790008 cites W2144857397 @default.
• W4240790008 cites W2146512944 @default.
• W4240790008 cites W2153593258 @default.
• W4240790008 cites W2169456326 @default.
• W4240790008 cites W2179438025 @default.
• W4240790008 cites W2272989235 @default.
• W4240790008 cites W2275381817 @default.
• W4240790008 cites W2276034383 @default.
• W4240790008 cites W2343186061 @default.
• W4240790008 cites W2460946499 @default.
• W4240790008 cites W2519279061 @default.
• W4240790008 cites W2570708526 @default.
• W4240790008 cites W2580396797 @default.
• W4240790008 cites W2584322210 @default.
• W4240790008 cites W2610356532 @default.
• W4240790008 cites W2759082725 @default.
• W4240790008 cites W2763354058 @default.
• W4240790008 cites W2766437506 @default.
• W4240790008 cites W2790016310 @default.
• W4240790008 cites W2793728773 @default.
• W4240790008 cites W2794480084 @default.
• W4240790008 cites W2802933453 @default.
• W4240790008 cites W2886296986 @default.
• W4240790008 cites W2908126987 @default.
• W4240790008 cites W2918701820 @default.
• W4240790008 cites W2943222525 @default.
• W4240790008 cites W2947244263 @default.
• W4240790008 cites W2949138297 @default.
• W4240790008 cites W2949380229 @default.
• W4240790008 cites W2950470413 @default.
• W4240790008 cites W2951277388 @default.
• W4240790008 cites W2953044679 @default.

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