Matches in SemOpenAlex for { <https://semopenalex.org/work/W4240824668> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W4240824668 abstract "Abstract BackgroundLDHA is overexpressed in many kinds of cancer, and associated with poor prognosis. In the current study, we evaluated the prognostic value of LDHA expression in non-small cell lung cancer (NSCLC), and tested whether LDHA inhibition might improve radiotherapy efficacy in NSCLC.MethodsLDHA mRNA expression was investigated in NSCLC patients, using online database and further verified by immunohistochemistry. The prognostic value of LDHA was evaluated using Kaplan-Meier plotter database. In vitro, two NSCLC cell lines were pretreated with oxamate, an inhibitor of LDHA, and colony formation method was performed to determine cellular radiosensitivity. Comet assay was used to detect DNA damage after irradiation. Flow cytometry was applied to test cell cycle progression and apoptosis, and autophagy was examined by monodansylcadaverin (MDC) staining.ResultsBoth mRNA and protein levels of LDHA expression were up-regulated in NSCLC tissues. High LDHA expression was a poor prognostic factor and associated with radioresistance in NSCLC patients. LDHA inhibition by oxamate remarkably increased radiosensitivity in both A549 and H1975 cancer cells, and enhanced ionizing radiation (IR)-induced apoptosis and autophagy, accompanied by cell cycle distribution alternations. Furthermore, LDHA inhibition induced reactive oxygen species (ROS) accumulation and cellular ATP depletion, which might increase DNA injury and hinder DNA repair activity.ConclusionsOur study suggests inhibition of LDHA may be a potential strategy to improve radiotherapy efficacy in NSCLC patients, which needs to be further tested by clinical trials." @default.
- W4240824668 created "2022-05-12" @default.
- W4240824668 creator A5004138615 @default.
- W4240824668 creator A5004823247 @default.
- W4240824668 creator A5005461513 @default.
- W4240824668 creator A5009239084 @default.
- W4240824668 creator A5019384060 @default.
- W4240824668 creator A5031746274 @default.
- W4240824668 creator A5038996995 @default.
- W4240824668 creator A5067404460 @default.
- W4240824668 date "2021-02-23" @default.
- W4240824668 modified "2023-10-14" @default.
- W4240824668 title "Targeting Lactate Dehydrogenase A Improves Radiotherapy Efficacy in Non-Small Cell Lung Cancer: From Bedside to Bench" @default.
- W4240824668 doi "https://doi.org/10.21203/rs.3.rs-222733/v1" @default.
- W4240824668 hasPublicationYear "2021" @default.
- W4240824668 type Work @default.
- W4240824668 citedByCount "1" @default.
- W4240824668 countsByYear W42408246682023 @default.
- W4240824668 crossrefType "posted-content" @default.
- W4240824668 hasAuthorship W4240824668A5004138615 @default.
- W4240824668 hasAuthorship W4240824668A5004823247 @default.
- W4240824668 hasAuthorship W4240824668A5005461513 @default.
- W4240824668 hasAuthorship W4240824668A5009239084 @default.
- W4240824668 hasAuthorship W4240824668A5019384060 @default.
- W4240824668 hasAuthorship W4240824668A5031746274 @default.
- W4240824668 hasAuthorship W4240824668A5038996995 @default.
- W4240824668 hasAuthorship W4240824668A5067404460 @default.
- W4240824668 hasBestOaLocation W42408246681 @default.
- W4240824668 hasConcept C121608353 @default.
- W4240824668 hasConcept C126322002 @default.
- W4240824668 hasConcept C143425029 @default.
- W4240824668 hasConcept C143998085 @default.
- W4240824668 hasConcept C181199279 @default.
- W4240824668 hasConcept C190283241 @default.
- W4240824668 hasConcept C206684579 @default.
- W4240824668 hasConcept C2776256026 @default.
- W4240824668 hasConcept C2777318727 @default.
- W4240824668 hasConcept C2780358455 @default.
- W4240824668 hasConcept C2910680157 @default.
- W4240824668 hasConcept C29537977 @default.
- W4240824668 hasConcept C502942594 @default.
- W4240824668 hasConcept C509974204 @default.
- W4240824668 hasConcept C53227056 @default.
- W4240824668 hasConcept C552990157 @default.
- W4240824668 hasConcept C55493867 @default.
- W4240824668 hasConcept C58962609 @default.
- W4240824668 hasConcept C71924100 @default.
- W4240824668 hasConcept C81729549 @default.
- W4240824668 hasConcept C86803240 @default.
- W4240824668 hasConcept C96232424 @default.
- W4240824668 hasConceptScore W4240824668C121608353 @default.
- W4240824668 hasConceptScore W4240824668C126322002 @default.
- W4240824668 hasConceptScore W4240824668C143425029 @default.
- W4240824668 hasConceptScore W4240824668C143998085 @default.
- W4240824668 hasConceptScore W4240824668C181199279 @default.
- W4240824668 hasConceptScore W4240824668C190283241 @default.
- W4240824668 hasConceptScore W4240824668C206684579 @default.
- W4240824668 hasConceptScore W4240824668C2776256026 @default.
- W4240824668 hasConceptScore W4240824668C2777318727 @default.
- W4240824668 hasConceptScore W4240824668C2780358455 @default.
- W4240824668 hasConceptScore W4240824668C2910680157 @default.
- W4240824668 hasConceptScore W4240824668C29537977 @default.
- W4240824668 hasConceptScore W4240824668C502942594 @default.
- W4240824668 hasConceptScore W4240824668C509974204 @default.
- W4240824668 hasConceptScore W4240824668C53227056 @default.
- W4240824668 hasConceptScore W4240824668C552990157 @default.
- W4240824668 hasConceptScore W4240824668C55493867 @default.
- W4240824668 hasConceptScore W4240824668C58962609 @default.
- W4240824668 hasConceptScore W4240824668C71924100 @default.
- W4240824668 hasConceptScore W4240824668C81729549 @default.
- W4240824668 hasConceptScore W4240824668C86803240 @default.
- W4240824668 hasConceptScore W4240824668C96232424 @default.
- W4240824668 hasLocation W42408246681 @default.
- W4240824668 hasLocation W42408246682 @default.
- W4240824668 hasOpenAccess W4240824668 @default.
- W4240824668 hasPrimaryLocation W42408246681 @default.
- W4240824668 hasRelatedWork W1596907138 @default.
- W4240824668 hasRelatedWork W2012917573 @default.
- W4240824668 hasRelatedWork W2028485403 @default.
- W4240824668 hasRelatedWork W2089826616 @default.
- W4240824668 hasRelatedWork W2159104816 @default.
- W4240824668 hasRelatedWork W2415398623 @default.
- W4240824668 hasRelatedWork W2516370945 @default.
- W4240824668 hasRelatedWork W2902302018 @default.
- W4240824668 hasRelatedWork W3012601909 @default.
- W4240824668 hasRelatedWork W2892891728 @default.
- W4240824668 isParatext "false" @default.
- W4240824668 isRetracted "false" @default.
- W4240824668 workType "article" @default.