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- W4240951437 abstract "<ns3:p><ns3:bold>Background:</ns3:bold> Accurate viral load (VL) determination is paramount to determine the efficacy of anti-HIV-1 therapy. The conventional method used, fit-point (FP), assumes an equal efficiency in the polymerase chain reaction (PCR) among samples that might not hold for low-input templates. An alternative approach, maxRatio, was introduced to compensate for inhibition in PCR.</ns3:p><ns3:p> <ns3:bold>Methods:</ns3:bold> Herein, we assessed whether maxRatio could improve VL quantification using 2,544 QIAgen artus HI virus-1 RT-PCR reactions. The assay’s standard dilutions were used to build external standard curves with either FP or maxRatio that re-calculated the VLs.</ns3:p><ns3:p> <ns3:bold>Results:</ns3:bold> FP and maxRatio were highly comparable (Pearson’s ρ=0.994, Cohen’s κ=0.885), and the combination of the two methods identified samples (n=41) with aberrant amplification profiles.</ns3:p><ns3:p> <ns3:bold>Conclusions:</ns3:bold> The combination of maxRatio and FP could improve the predictive value of the assay.</ns3:p>" @default.
- W4240951437 created "2022-05-12" @default.
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- W4240951437 date "2021-06-29" @default.
- W4240951437 modified "2023-10-14" @default.
- W4240951437 title "maxRatio improves the detection of samples with abnormal amplification profiles on QIAgen’s artus HIV-1 qPCR assay" @default.
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- W4240951437 doi "https://doi.org/10.12688/f1000research.25738.2" @default.
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