Matches in SemOpenAlex for { <https://semopenalex.org/work/W4241258884> ?p ?o ?g. }
Showing items 1 to 50 of
50
with 100 items per page.
- W4241258884 endingPage "539" @default.
- W4241258884 startingPage "535" @default.
- W4241258884 abstract "The Edman degradation is a series of chemical reactions that sequentially removes N-terminal amino acids from a peptide or protein. The overall reaction sequence is shown in Fig. 1. In the first step (the coupling reaction) phenylisothocyanate (PITC) reacts with the N-terminal amino group of the peptide or protein. The sample is then dried and treated with an anhydrous acid (e.g., trifluoracetic acid), which results in cleavage of the peptide bond between the first and second amino acid. The N-terminal amino acids is therefore released as a derivative (the thiazolinone). The thiazolinone is extracted into an organic solvent, dried down, and then converted to the more stable phenylthiohydantoin (PTH) derivative (the conversion step). The PTH amino acid is then identified, normally by reverse-phase HPLC. This is known as the direct Edman degradation and is, for example, the method used in an automated sequencing machine. The dansyl-Edman method for peptide sequencing described here is based on the Edman degradation, but with the following modifications. Following the cleavage step the thiazolinone is extracted, but rather than being converted to the PTH derivative it is discarded. Instead, a small fraction (5%) of the remaining peptide is taken and the newly liberated N-terminal amino acid determined in this sample by the dansyl method (see Chapter 89). Although the dansyl-Edman method results in successively less peptide being present at each cycle of the Edman degradation, this loss of material is compensated for by the considerable sensitivity of the dansyl method for identifying N-terminal amino acids. The dansyl-Edman method described here was originally introduced by Hartley (1)." @default.
- W4241258884 created "2022-05-12" @default.
- W4241258884 creator A5054773163 @default.
- W4241258884 date "1996-01-01" @default.
- W4241258884 modified "2023-09-25" @default.
- W4241258884 title "The Dansyl-Edman Method for Peptide Sequencing" @default.
- W4241258884 cites W184399086 @default.
- W4241258884 cites W2020613768 @default.
- W4241258884 doi "https://doi.org/10.1007/978-1-60327-259-9_90" @default.
- W4241258884 hasPublicationYear "1996" @default.
- W4241258884 type Work @default.
- W4241258884 citedByCount "1" @default.
- W4241258884 crossrefType "book-chapter" @default.
- W4241258884 hasAuthorship W4241258884A5054773163 @default.
- W4241258884 hasConcept C104317684 @default.
- W4241258884 hasConcept C167625842 @default.
- W4241258884 hasConcept C185592680 @default.
- W4241258884 hasConcept C2779281246 @default.
- W4241258884 hasConcept C31684184 @default.
- W4241258884 hasConcept C43617362 @default.
- W4241258884 hasConcept C515207424 @default.
- W4241258884 hasConcept C55493867 @default.
- W4241258884 hasConcept C87933860 @default.
- W4241258884 hasConceptScore W4241258884C104317684 @default.
- W4241258884 hasConceptScore W4241258884C167625842 @default.
- W4241258884 hasConceptScore W4241258884C185592680 @default.
- W4241258884 hasConceptScore W4241258884C2779281246 @default.
- W4241258884 hasConceptScore W4241258884C31684184 @default.
- W4241258884 hasConceptScore W4241258884C43617362 @default.
- W4241258884 hasConceptScore W4241258884C515207424 @default.
- W4241258884 hasConceptScore W4241258884C55493867 @default.
- W4241258884 hasConceptScore W4241258884C87933860 @default.
- W4241258884 hasLocation W42412588841 @default.
- W4241258884 hasOpenAccess W4241258884 @default.
- W4241258884 hasPrimaryLocation W42412588841 @default.
- W4241258884 hasRelatedWork W2016062340 @default.
- W4241258884 hasRelatedWork W2020644290 @default.
- W4241258884 hasRelatedWork W2035457379 @default.
- W4241258884 hasRelatedWork W2409857678 @default.
- W4241258884 hasRelatedWork W2440069514 @default.
- W4241258884 hasRelatedWork W2950555525 @default.
- W4241258884 hasRelatedWork W3140259171 @default.
- W4241258884 hasRelatedWork W3213245720 @default.
- W4241258884 hasRelatedWork W421199967 @default.
- W4241258884 hasRelatedWork W4255945608 @default.
- W4241258884 isParatext "false" @default.
- W4241258884 isRetracted "false" @default.
- W4241258884 workType "book-chapter" @default.