Matches in SemOpenAlex for { <https://semopenalex.org/work/W4241736772> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W4241736772 endingPage "865" @default.
- W4241736772 startingPage "856" @default.
- W4241736772 abstract "Nucleation of cholesterol monohydrate crystals from bile is a critical step in the formation of cholesterol gallstones. Measurement of nucleation in model bile systems and the characteristics of the initial nucleus have proven elusive. In this study we have used three separate physical chemical techniques to examine vesicle aggregation and fusion, including dynamic light scattering (DLS), transmission electron microscopy (TEM), and fluorescent biochemical assays. These assays enabled us to quantify the effect of biliary proteins, such as gallbladder mucin, on vesicle fusion and aggregation. In the absence of mucin, fusion is a relatively slow process occurring over 24 hours, whereas physiological concentrations of mucin are able to accelerate almost complete fusion of vesicles within 6 hours. Vesicle fusion and aggregation as characterized by TEM result in the formation of aggregates of multilamellar vesicles and giant fusion bodies associated with a background of mucin. These mucin-vesicle aggregate bodies may represent true nuclei and precede cholesterol monohydrate crystal nucleation. In future studies, these vesicle fusion assays can be used to quantitatively examine the effect of putative pro- and anti-nucleating proteins on the earliest steps of cholesterol crystal nucleation." @default.
- W4241736772 created "2022-05-12" @default.
- W4241736772 creator A5009926376 @default.
- W4241736772 date "1995-09-01" @default.
- W4241736772 modified "2023-09-26" @default.
- W4241736772 title "Mucin-vesicle interactions in model bile: Evidence for vesicle aggregation and fusion before cholesterol crystal formation*1, *2" @default.
- W4241736772 doi "https://doi.org/10.1016/0270-9139(95)90308-9" @default.
- W4241736772 hasPublicationYear "1995" @default.
- W4241736772 type Work @default.
- W4241736772 citedByCount "2" @default.
- W4241736772 crossrefType "journal-article" @default.
- W4241736772 hasAuthorship W4241736772A5009926376 @default.
- W4241736772 hasBestOaLocation W42417367721 @default.
- W4241736772 hasConcept C103038307 @default.
- W4241736772 hasConcept C12554922 @default.
- W4241736772 hasConcept C130316041 @default.
- W4241736772 hasConcept C138885662 @default.
- W4241736772 hasConcept C148785051 @default.
- W4241736772 hasConcept C158525013 @default.
- W4241736772 hasConcept C178790620 @default.
- W4241736772 hasConcept C179264091 @default.
- W4241736772 hasConcept C185154212 @default.
- W4241736772 hasConcept C185592680 @default.
- W4241736772 hasConcept C196330066 @default.
- W4241736772 hasConcept C2778163477 @default.
- W4241736772 hasConcept C41625074 @default.
- W4241736772 hasConcept C41895202 @default.
- W4241736772 hasConcept C55493867 @default.
- W4241736772 hasConcept C61048295 @default.
- W4241736772 hasConcept C8010536 @default.
- W4241736772 hasConcept C86803240 @default.
- W4241736772 hasConceptScore W4241736772C103038307 @default.
- W4241736772 hasConceptScore W4241736772C12554922 @default.
- W4241736772 hasConceptScore W4241736772C130316041 @default.
- W4241736772 hasConceptScore W4241736772C138885662 @default.
- W4241736772 hasConceptScore W4241736772C148785051 @default.
- W4241736772 hasConceptScore W4241736772C158525013 @default.
- W4241736772 hasConceptScore W4241736772C178790620 @default.
- W4241736772 hasConceptScore W4241736772C179264091 @default.
- W4241736772 hasConceptScore W4241736772C185154212 @default.
- W4241736772 hasConceptScore W4241736772C185592680 @default.
- W4241736772 hasConceptScore W4241736772C196330066 @default.
- W4241736772 hasConceptScore W4241736772C2778163477 @default.
- W4241736772 hasConceptScore W4241736772C41625074 @default.
- W4241736772 hasConceptScore W4241736772C41895202 @default.
- W4241736772 hasConceptScore W4241736772C55493867 @default.
- W4241736772 hasConceptScore W4241736772C61048295 @default.
- W4241736772 hasConceptScore W4241736772C8010536 @default.
- W4241736772 hasConceptScore W4241736772C86803240 @default.
- W4241736772 hasIssue "3" @default.
- W4241736772 hasLocation W42417367721 @default.
- W4241736772 hasOpenAccess W4241736772 @default.
- W4241736772 hasPrimaryLocation W42417367721 @default.
- W4241736772 hasRelatedWork W1971369150 @default.
- W4241736772 hasRelatedWork W1981926458 @default.
- W4241736772 hasRelatedWork W1982379525 @default.
- W4241736772 hasRelatedWork W2008976522 @default.
- W4241736772 hasRelatedWork W2056542147 @default.
- W4241736772 hasRelatedWork W2062367641 @default.
- W4241736772 hasRelatedWork W2067703043 @default.
- W4241736772 hasRelatedWork W2124302258 @default.
- W4241736772 hasRelatedWork W2792446218 @default.
- W4241736772 hasRelatedWork W2913640882 @default.
- W4241736772 hasVolume "22" @default.
- W4241736772 isParatext "false" @default.
- W4241736772 isRetracted "false" @default.
- W4241736772 workType "article" @default.