Matches in SemOpenAlex for { <https://semopenalex.org/work/W4241805017> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W4241805017 endingPage "104" @default.
- W4241805017 startingPage "89" @default.
- W4241805017 abstract "Human embryonic stem cells (hESC) involve long-term cultures that must remain undifferentiated. The real-time PCR (RT-PCR) technique allows the relative quantification of target genes, including undifferentiation and differentiation markers when referred to a housekeeping control with the addition of a calibrator that serves as an internal control to compare different lots of reactions during the time. The main aspects will include a minimal number of cells to be analyzed, genes to be tested, and how to choose the appropriate calibrator sample and the reference gene. In this chapter, we present how to apply the RT-PCR technique, protocols for its performance, experimental setup, and software analysis, as of the gene expression of hESC lines in consecutive passages for long-term culture surveillance." @default.
- W4241805017 created "2022-05-12" @default.
- W4241805017 creator A5011157499 @default.
- W4241805017 creator A5090345459 @default.
- W4241805017 date "2014-01-01" @default.
- W4241805017 modified "2023-10-17" @default.
- W4241805017 title "Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR" @default.
- W4241805017 cites W1986555888 @default.
- W4241805017 cites W2008083733 @default.
- W4241805017 cites W2024200662 @default.
- W4241805017 cites W2043816356 @default.
- W4241805017 cites W2093613096 @default.
- W4241805017 cites W2094557730 @default.
- W4241805017 cites W2101556559 @default.
- W4241805017 cites W2110301828 @default.
- W4241805017 cites W2120920194 @default.
- W4241805017 cites W2130953036 @default.
- W4241805017 cites W2135598172 @default.
- W4241805017 cites W2140451732 @default.
- W4241805017 cites W2160619422 @default.
- W4241805017 cites W2187113859 @default.
- W4241805017 cites W2418944125 @default.
- W4241805017 doi "https://doi.org/10.1007/7651_2014_131" @default.
- W4241805017 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25403464" @default.
- W4241805017 hasPublicationYear "2014" @default.
- W4241805017 type Work @default.
- W4241805017 citedByCount "0" @default.
- W4241805017 crossrefType "book-chapter" @default.
- W4241805017 hasAuthorship W4241805017A5011157499 @default.
- W4241805017 hasAuthorship W4241805017A5090345459 @default.
- W4241805017 hasConcept C104317684 @default.
- W4241805017 hasConcept C121332964 @default.
- W4241805017 hasConcept C145103041 @default.
- W4241805017 hasConcept C150194340 @default.
- W4241805017 hasConcept C153911025 @default.
- W4241805017 hasConcept C33922130 @default.
- W4241805017 hasConcept C41008148 @default.
- W4241805017 hasConcept C48023723 @default.
- W4241805017 hasConcept C54355233 @default.
- W4241805017 hasConcept C61797465 @default.
- W4241805017 hasConcept C62520636 @default.
- W4241805017 hasConcept C70721500 @default.
- W4241805017 hasConcept C86803240 @default.
- W4241805017 hasConcept C90861158 @default.
- W4241805017 hasConceptScore W4241805017C104317684 @default.
- W4241805017 hasConceptScore W4241805017C121332964 @default.
- W4241805017 hasConceptScore W4241805017C145103041 @default.
- W4241805017 hasConceptScore W4241805017C150194340 @default.
- W4241805017 hasConceptScore W4241805017C153911025 @default.
- W4241805017 hasConceptScore W4241805017C33922130 @default.
- W4241805017 hasConceptScore W4241805017C41008148 @default.
- W4241805017 hasConceptScore W4241805017C48023723 @default.
- W4241805017 hasConceptScore W4241805017C54355233 @default.
- W4241805017 hasConceptScore W4241805017C61797465 @default.
- W4241805017 hasConceptScore W4241805017C62520636 @default.
- W4241805017 hasConceptScore W4241805017C70721500 @default.
- W4241805017 hasConceptScore W4241805017C86803240 @default.
- W4241805017 hasConceptScore W4241805017C90861158 @default.
- W4241805017 hasLocation W42418050171 @default.
- W4241805017 hasLocation W42418050172 @default.
- W4241805017 hasOpenAccess W4241805017 @default.
- W4241805017 hasPrimaryLocation W42418050171 @default.
- W4241805017 hasRelatedWork W1963740134 @default.
- W4241805017 hasRelatedWork W2069386104 @default.
- W4241805017 hasRelatedWork W2165810656 @default.
- W4241805017 hasRelatedWork W2499641533 @default.
- W4241805017 hasRelatedWork W2772773964 @default.
- W4241805017 hasRelatedWork W3032293786 @default.
- W4241805017 hasRelatedWork W3129904083 @default.
- W4241805017 hasRelatedWork W4236341438 @default.
- W4241805017 hasRelatedWork W4362204953 @default.
- W4241805017 hasRelatedWork W2004603282 @default.
- W4241805017 isParatext "false" @default.
- W4241805017 isRetracted "false" @default.
- W4241805017 workType "book-chapter" @default.