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- W4241943428 abstract "Stallion semen is commonly cryopreserved in 0.5 ml straws at a concentration of 200-400 million sperm per milliliter. Traditional artificial insemination with frozen semen may use one to eight straws per dose. Intracytoplasmic sperm injection (ICSI) has made it possible to produce embryos and subsequent pregnancies utilizing a single motile sperm. Several strategies have been investigated as to how to optimize the use of frozen semen for ICSI. Stallion semen initially frozen in one cryopreservation extender can be thawed and diluted in a different extender and refrozen without significant detriment to the spermatozoa. Even the small number of motile spermatozoa obtained after thawing a small piece of a frozen straw can be diluted in freezing extender and refrozen." @default.
- W4241943428 created "2022-05-12" @default.
- W4241943428 creator A5073248194 @default.
- W4241943428 date "2021-02-19" @default.
- W4241943428 modified "2023-10-16" @default.
- W4241943428 title "Refreezing Semen" @default.
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- W4241943428 doi "https://doi.org/10.1002/9781119556015.ch154" @default.
- W4241943428 hasPublicationYear "2021" @default.
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