FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4241946216> ?p ?o ?g. }

• W4241946216 abstract "Event Abstract Back to Event Surface expression of human Respiratory Syncytial Virus (hRSV) Nucleoprotein inhibits immunological synapse assembly by T cells Pablo F. Céspedes1, 2*, Susan M. Bueno1, 2, Bruno A. Ramirez1, 2, Roberto S. Gómez1, 2, Sebastián A. Riquelme1, 2, 3, Christian E. Palavecino1, 2, Juan P. Mackern-Oberti1, 2, Jorge Mora1, 2, David Depoil4, 5, Catarina Sacristán4, Michael Cammer4, Alison Creneguy3, Tuan H. Nguyen3, Claudia A. Riedel2, 6, Michael L. Dustin4, 5 and Alexis M. Kalergis1, 2, 7* 1 Pontificia Universidad Católica de Chile, Facultad de Ciencias Biológicas, Departamento de Genética Molecular y Microbiología, Chile 2 Millennium Institute on Immunology and Immunotherapy, Chile 3 INSERM, U1064, France 4 New York University, Helen and Martin Kimmel Center for Biology and Medicine, Skirball Institute of Biomolecular Medicine, United States 5 University of Oxford, Kennedy Institute of Rheumatology, United Kingdom 6 Universidad Nacional Andrés Bello, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas y Facultad de Medicina,, Chile 7 Pontificia Universidad Católica de Chile, Facultad de Medicina, Departamento de Inmunología Clínica y Reumatología, Chile The human Respiratory Syncytial Virus (hRSV) is the major cause of children severe respiratory tract infections worldwide. Both, the poor activation of T cells and the limited generation of memory T cells following disease resolution are considered key processes favoring hRSV epidemics, which are based on re-infections. It is known that hRSV infects Dendritic cells (DCs) dampening their capacity to activate naïve T cells, a critical process in the initiation, expansion and establishment of proper antiviral immune responses. Here we show that the hRSV nucleoprotein (N) is expressed at the surface of a variety of infected cells, including DCs. After estimating the approximate density of N in the surface of DCs and other cells, we evaluated whether these physiological densities could affect the assembly of the immunological synapse (IS) by naïve and antigen-experienced CD4+ T cells. We observed that N, but not control proteins inhibited the assembly of IS by interfering with TCR-pMHC interactions, which was accompanied with reduced accumulation of TCR and reduced central clustering of pMHC at the T cell-bilayer interface. As expected, reduced synapse assembly, as well as reduced pMHC and TCR accumulation was associated with a consequent reduction in the phosphorylation of tyrosine residues in the interacting surface of the stimulated T cells. Similarly, either DCs transduced with lentivirus encoding hRSV N or DCs loaded with purified N protein failed at inducing the up-regulation of the TCR-driven activation marker CD69 in stimulated T cells. Altogether, our findings propose N-hRSV as a key virulence factor responsible for the suboptimal T cell activation observed in hRSV-infected individuals and the poor acquisition of memory T cell responses following disease resolution. Acknowledgements This research was supported by CONICYT grants FONDECYT POSTDOCTORADO N° 3140455, and by FONDECYT grant 1070352; FONDEF D061008; and the Millennium Institute on Immunology and Immunotherapy (P07/088-F) and grant “Nouvelles Equipes-nouvelles thématiques” from the La Région Pays De La Loire and the Evaluation-Orientation of Scientific Cooperation France-Chile grant. Keywords: T cell priming, memory T cells, TCR signaling, cSMAC, pSMAC, Nucleocapsid protein, Ribonucleoproteins, Human Respiratory Syncytial Virus A Conference: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015. Presentation Type: Oral Presentation Topic: Adaptive Immunity Citation: Céspedes PF, Bueno SM, Ramirez BA, Gómez RS, Riquelme SA, Palavecino CE, Mackern-Oberti JP, Mora J, Depoil D, Sacristán C, Cammer M, Creneguy A, Nguyen TH, Riedel CA, Dustin ML and Kalergis AM (2015). Surface expression of human Respiratory Syncytial Virus (hRSV) Nucleoprotein inhibits immunological synapse assembly by T cells. Front. Immunol. Conference Abstract: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología. doi: 10.3389/conf.fimmu.2015.05.00315 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 15 Apr 2015; Published Online: 15 Sep 2015. * Correspondence: Dr. Pablo F Céspedes, Pontificia Universidad Católica de Chile, Facultad de Ciencias Biológicas, Departamento de Genética Molecular y Microbiología, Santiago, RM, 8331150, Chile, pfcespedes@icloud.com Dr. Alexis M Kalergis, Pontificia Universidad Católica de Chile, Facultad de Ciencias Biológicas, Departamento de Genética Molecular y Microbiología, Santiago, RM, 8331150, Chile, akalergis@bio.puc.cl Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Pablo F Céspedes Susan M Bueno Bruno A Ramirez Roberto S Gómez Sebastián A Riquelme Christian E Palavecino Juan P Mackern-Oberti Jorge Mora David Depoil Catarina Sacristán Michael Cammer Alison Creneguy Tuan H Nguyen Claudia A Riedel Michael L Dustin Alexis M Kalergis Google Pablo F Céspedes Susan M Bueno Bruno A Ramirez Roberto S Gómez Sebastián A Riquelme Christian E Palavecino Juan P Mackern-Oberti Jorge Mora David Depoil Catarina Sacristán Michael Cammer Alison Creneguy Tuan H Nguyen Claudia A Riedel Michael L Dustin Alexis M Kalergis Google Scholar Pablo F Céspedes Susan M Bueno Bruno A Ramirez Roberto S Gómez Sebastián A Riquelme Christian E Palavecino Juan P Mackern-Oberti Jorge Mora David Depoil Catarina Sacristán Michael Cammer Alison Creneguy Tuan H Nguyen Claudia A Riedel Michael L Dustin Alexis M Kalergis PubMed Pablo F Céspedes Susan M Bueno Bruno A Ramirez Roberto S Gómez Sebastián A Riquelme Christian E Palavecino Juan P Mackern-Oberti Jorge Mora David Depoil Catarina Sacristán Michael Cammer Alison Creneguy Tuan H Nguyen Claudia A Riedel Michael L Dustin Alexis M Kalergis Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page." @default.
• W4241946216 created "2022-05-12" @default.
• W4241946216 creator A5002224002 @default.
• W4241946216 creator A5010249723 @default.
• W4241946216 creator A5013958038 @default.
• W4241946216 creator A5016214784 @default.
• W4241946216 creator A5037758363 @default.
• W4241946216 creator A5038082842 @default.
• W4241946216 creator A5049474292 @default.
• W4241946216 creator A5054172921 @default.
• W4241946216 creator A5062812641 @default.
• W4241946216 creator A5064674643 @default.
• W4241946216 creator A5071731581 @default.
• W4241946216 creator A5073585696 @default.
• W4241946216 creator A5073890799 @default.
• W4241946216 creator A5079206508 @default.
• W4241946216 creator A5089051377 @default.
• W4241946216 creator A5091892078 @default.
• W4241946216 date "2015-01-01" @default.
• W4241946216 modified "2023-10-14" @default.
• W4241946216 title "Surface expression of human Respiratory Syncytial Virus (hRSV) Nucleoprotein inhibits immunological synapse assembly by T cells" @default.
• W4241946216 doi "https://doi.org/10.3389/conf.fimmu.2015.05.00315" @default.
• W4241946216 hasPublicationYear "2015" @default.
• W4241946216 type Work @default.
• W4241946216 citedByCount "0" @default.
• W4241946216 crossrefType "journal-article" @default.
• W4241946216 hasAuthorship W4241946216A5002224002 @default.
• W4241946216 hasAuthorship W4241946216A5010249723 @default.
• W4241946216 hasAuthorship W4241946216A5013958038 @default.
• W4241946216 hasAuthorship W4241946216A5016214784 @default.
• W4241946216 hasAuthorship W4241946216A5037758363 @default.
• W4241946216 hasAuthorship W4241946216A5038082842 @default.
• W4241946216 hasAuthorship W4241946216A5049474292 @default.
• W4241946216 hasAuthorship W4241946216A5054172921 @default.
• W4241946216 hasAuthorship W4241946216A5062812641 @default.
• W4241946216 hasAuthorship W4241946216A5064674643 @default.
• W4241946216 hasAuthorship W4241946216A5071731581 @default.
• W4241946216 hasAuthorship W4241946216A5073585696 @default.
• W4241946216 hasAuthorship W4241946216A5073890799 @default.
• W4241946216 hasAuthorship W4241946216A5079206508 @default.
• W4241946216 hasAuthorship W4241946216A5089051377 @default.
• W4241946216 hasAuthorship W4241946216A5091892078 @default.
• W4241946216 hasBestOaLocation W42419462161 @default.
• W4241946216 hasConcept C105702510 @default.
• W4241946216 hasConcept C159047783 @default.
• W4241946216 hasConcept C19317047 @default.
• W4241946216 hasConcept C203014093 @default.
• W4241946216 hasConcept C2522874641 @default.
• W4241946216 hasConcept C2776090121 @default.
• W4241946216 hasConcept C534529494 @default.
• W4241946216 hasConcept C60325843 @default.
• W4241946216 hasConcept C86803240 @default.
• W4241946216 hasConcept C8891405 @default.
• W4241946216 hasConcept C95444343 @default.
• W4241946216 hasConcept C9756297 @default.
• W4241946216 hasConceptScore W4241946216C105702510 @default.
• W4241946216 hasConceptScore W4241946216C159047783 @default.
• W4241946216 hasConceptScore W4241946216C19317047 @default.
• W4241946216 hasConceptScore W4241946216C203014093 @default.
• W4241946216 hasConceptScore W4241946216C2522874641 @default.
• W4241946216 hasConceptScore W4241946216C2776090121 @default.
• W4241946216 hasConceptScore W4241946216C534529494 @default.
• W4241946216 hasConceptScore W4241946216C60325843 @default.
• W4241946216 hasConceptScore W4241946216C86803240 @default.
• W4241946216 hasConceptScore W4241946216C8891405 @default.
• W4241946216 hasConceptScore W4241946216C95444343 @default.
• W4241946216 hasConceptScore W4241946216C9756297 @default.
• W4241946216 hasLocation W42419462161 @default.
• W4241946216 hasOpenAccess W4241946216 @default.
• W4241946216 hasPrimaryLocation W42419462161 @default.
• W4241946216 hasRelatedWork W1487857142 @default.
• W4241946216 hasRelatedWork W1590027774 @default.
• W4241946216 hasRelatedWork W1989353793 @default.
• W4241946216 hasRelatedWork W2020314332 @default.
• W4241946216 hasRelatedWork W2100196824 @default.
• W4241946216 hasRelatedWork W2151148014 @default.
• W4241946216 hasRelatedWork W2172096973 @default.
• W4241946216 hasRelatedWork W2313303031 @default.
• W4241946216 hasRelatedWork W2612443549 @default.
• W4241946216 hasRelatedWork W3003494581 @default.
• W4241946216 hasVolume "6" @default.
• W4241946216 isParatext "false" @default.
• W4241946216 isRetracted "false" @default.
• W4241946216 workType "article" @default.