Matches in SemOpenAlex for { <https://semopenalex.org/work/W4242075156> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W4242075156 abstract "Abstract Background: Ticks are blood sucking ectoparasites that play a pivotal role in the transmission of various pathogens to humans and animals. In Korea, Haemaphysalis longicornis is the predominant tick species and is recognized as the vector of pathogens causing various diseases such as babesiosis, borreliosis, rickettsiosis, and severe fever with thrombocytopenia syndrome. Methods: In this study, we developed a method to screen bacterial pathogens in H. longicornis using targeted high-throughput sequencing of the 16S rRNA V4 region using the state-of-the-art sequencing instrument, iSeq 100, and compared the findings with those of conventional PCR with specific primers. Results: Rickettsia spp. were detected in 18 out of 39 samples using iSeq 100 but in only 14 samples using conventional PCR. In the phylogenetic analysis using gltA and ompA sequences of the detected Rickettsia , the highest sequence similarity was found with Candidatus Rickettsia jingxinensis isolate Xian-Hl-79, Ca. R. jingxinensis isolate F18, and Ca. R. longicornii isolate ROK-HL727. In the microbiome study, Coxiella AB001519, a known tick symbiont, was detected in all 39 tick samples. The Actinomycetospora chiangmaiensis group was more abundant in Rickettsia spp.-positive samples than in Rickettsia spp.-negative samples. Conclusions: Thus, we demonstrated that iSeq100 can rapidly and economically screen potential pathogens in ticks and can be applied to large-scale pathogen screening in arthropods for vector-borne disease control programs." @default.
- W4242075156 created "2022-05-12" @default.
- W4242075156 creator A5025766470 @default.
- W4242075156 creator A5037854128 @default.
- W4242075156 creator A5046445389 @default.
- W4242075156 creator A5059778316 @default.
- W4242075156 date "2021-04-14" @default.
- W4242075156 modified "2023-09-27" @default.
- W4242075156 title "iSeq100 for metagenomic pathogen screening in ticks" @default.
- W4242075156 doi "https://doi.org/10.21203/rs.3.rs-400674/v1" @default.
- W4242075156 hasPublicationYear "2021" @default.
- W4242075156 type Work @default.
- W4242075156 citedByCount "0" @default.
- W4242075156 crossrefType "posted-content" @default.
- W4242075156 hasAuthorship W4242075156A5025766470 @default.
- W4242075156 hasAuthorship W4242075156A5037854128 @default.
- W4242075156 hasAuthorship W4242075156A5046445389 @default.
- W4242075156 hasAuthorship W4242075156A5059778316 @default.
- W4242075156 hasBestOaLocation W42420751561 @default.
- W4242075156 hasConcept C104317684 @default.
- W4242075156 hasConcept C159047783 @default.
- W4242075156 hasConcept C2522874641 @default.
- W4242075156 hasConcept C2776005033 @default.
- W4242075156 hasConcept C2776127032 @default.
- W4242075156 hasConcept C2776460866 @default.
- W4242075156 hasConcept C2776607032 @default.
- W4242075156 hasConcept C2778434417 @default.
- W4242075156 hasConcept C2779420972 @default.
- W4242075156 hasConcept C2779620486 @default.
- W4242075156 hasConcept C2780147307 @default.
- W4242075156 hasConcept C2780414542 @default.
- W4242075156 hasConcept C40767141 @default.
- W4242075156 hasConcept C42062724 @default.
- W4242075156 hasConcept C523546767 @default.
- W4242075156 hasConcept C54355233 @default.
- W4242075156 hasConcept C86803240 @default.
- W4242075156 hasConcept C89423630 @default.
- W4242075156 hasConcept C92087593 @default.
- W4242075156 hasConceptScore W4242075156C104317684 @default.
- W4242075156 hasConceptScore W4242075156C159047783 @default.
- W4242075156 hasConceptScore W4242075156C2522874641 @default.
- W4242075156 hasConceptScore W4242075156C2776005033 @default.
- W4242075156 hasConceptScore W4242075156C2776127032 @default.
- W4242075156 hasConceptScore W4242075156C2776460866 @default.
- W4242075156 hasConceptScore W4242075156C2776607032 @default.
- W4242075156 hasConceptScore W4242075156C2778434417 @default.
- W4242075156 hasConceptScore W4242075156C2779420972 @default.
- W4242075156 hasConceptScore W4242075156C2779620486 @default.
- W4242075156 hasConceptScore W4242075156C2780147307 @default.
- W4242075156 hasConceptScore W4242075156C2780414542 @default.
- W4242075156 hasConceptScore W4242075156C40767141 @default.
- W4242075156 hasConceptScore W4242075156C42062724 @default.
- W4242075156 hasConceptScore W4242075156C523546767 @default.
- W4242075156 hasConceptScore W4242075156C54355233 @default.
- W4242075156 hasConceptScore W4242075156C86803240 @default.
- W4242075156 hasConceptScore W4242075156C89423630 @default.
- W4242075156 hasConceptScore W4242075156C92087593 @default.
- W4242075156 hasLocation W42420751561 @default.
- W4242075156 hasLocation W42420751562 @default.
- W4242075156 hasOpenAccess W4242075156 @default.
- W4242075156 hasPrimaryLocation W42420751561 @default.
- W4242075156 hasRelatedWork W10225528 @default.
- W4242075156 hasRelatedWork W15302533 @default.
- W4242075156 hasRelatedWork W23212264 @default.
- W4242075156 hasRelatedWork W32271426 @default.
- W4242075156 hasRelatedWork W36243659 @default.
- W4242075156 hasRelatedWork W36571330 @default.
- W4242075156 hasRelatedWork W41745555 @default.
- W4242075156 hasRelatedWork W6661793 @default.
- W4242075156 hasRelatedWork W7994175 @default.
- W4242075156 hasRelatedWork W9310743 @default.
- W4242075156 isParatext "false" @default.
- W4242075156 isRetracted "false" @default.
- W4242075156 workType "article" @default.