Matches in SemOpenAlex for { <https://semopenalex.org/work/W4242077314> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W4242077314 endingPage "314" @default.
- W4242077314 startingPage "305" @default.
- W4242077314 abstract "The transforming activity of cloned Moloney sarcoma virus (MSV) proviral DNA was inhibited by in vitro methylation of the DNA at cytosine residues, using HpaII and HhaI methylases before transfection into NIH 3T3 cells. The inhibition of transforming activity due to HpaII methylation was reversed by treatment of the transfected cells with 5-azacytidine, a specific inhibitor of methylation. Analysis of the genomic DNA from the transformed cells which resulted from the transfection of methylated MSV DNA revealed that the integrated MSV proviral DNA was sensitive to HpaII digestion in all cell lines examined, suggesting that loss of methyl groups was necessary for transformation. When cells were infected with Moloney murine leukemia virus at various times after transfection with methylated MSV DNA, the amount of transforming virus produced indicated that the loss of methyl groups occurred within 24 h. Methylation of MSV DNA at HhaI sites was as inhibitory to transforming activity as methylation at HpaII sites. In addition, methylation at both HpaII and HhaI sites did not further reduce the transforming activity of the DNA. These results suggested that; whereas methylation of specific sites on the provirus may not be essential for inhibiting the transforming activity of MSV DNA, methylation of specific regions may be necessary. Thus, by cotransfection of plasmids containing only specific regions of the MSV provirus, it was determined that methylation of the v-mos gene was more inhibitory to transformation than methylation of the viral long terminal repeat." @default.
- W4242077314 created "2022-05-12" @default.
- W4242077314 creator A5066807407 @default.
- W4242077314 creator A5081346761 @default.
- W4242077314 creator A5084705049 @default.
- W4242077314 creator A5091344747 @default.
- W4242077314 date "1983-03-01" @default.
- W4242077314 modified "2023-09-26" @default.
- W4242077314 title "In vitro methylation of specific regions of the cloned Moloney sarcoma virus genome inhibits its transforming activity." @default.
- W4242077314 doi "https://doi.org/10.1128/mcb.3.3.305" @default.
- W4242077314 hasPublicationYear "1983" @default.
- W4242077314 type Work @default.
- W4242077314 citedByCount "26" @default.
- W4242077314 crossrefType "journal-article" @default.
- W4242077314 hasAuthorship W4242077314A5066807407 @default.
- W4242077314 hasAuthorship W4242077314A5081346761 @default.
- W4242077314 hasAuthorship W4242077314A5084705049 @default.
- W4242077314 hasAuthorship W4242077314A5091344747 @default.
- W4242077314 hasBestOaLocation W42420773141 @default.
- W4242077314 hasConcept C102906071 @default.
- W4242077314 hasConcept C104317684 @default.
- W4242077314 hasConcept C141231307 @default.
- W4242077314 hasConcept C150194340 @default.
- W4242077314 hasConcept C153911025 @default.
- W4242077314 hasConcept C190727270 @default.
- W4242077314 hasConcept C22744801 @default.
- W4242077314 hasConcept C2777162945 @default.
- W4242077314 hasConcept C33288867 @default.
- W4242077314 hasConcept C54009773 @default.
- W4242077314 hasConcept C552990157 @default.
- W4242077314 hasConcept C55493867 @default.
- W4242077314 hasConcept C86803240 @default.
- W4242077314 hasConceptScore W4242077314C102906071 @default.
- W4242077314 hasConceptScore W4242077314C104317684 @default.
- W4242077314 hasConceptScore W4242077314C141231307 @default.
- W4242077314 hasConceptScore W4242077314C150194340 @default.
- W4242077314 hasConceptScore W4242077314C153911025 @default.
- W4242077314 hasConceptScore W4242077314C190727270 @default.
- W4242077314 hasConceptScore W4242077314C22744801 @default.
- W4242077314 hasConceptScore W4242077314C2777162945 @default.
- W4242077314 hasConceptScore W4242077314C33288867 @default.
- W4242077314 hasConceptScore W4242077314C54009773 @default.
- W4242077314 hasConceptScore W4242077314C552990157 @default.
- W4242077314 hasConceptScore W4242077314C55493867 @default.
- W4242077314 hasConceptScore W4242077314C86803240 @default.
- W4242077314 hasIssue "3" @default.
- W4242077314 hasLocation W42420773141 @default.
- W4242077314 hasLocation W42420773142 @default.
- W4242077314 hasLocation W42420773143 @default.
- W4242077314 hasOpenAccess W4242077314 @default.
- W4242077314 hasPrimaryLocation W42420773141 @default.
- W4242077314 hasRelatedWork W1993354505 @default.
- W4242077314 hasRelatedWork W2063359241 @default.
- W4242077314 hasRelatedWork W2072526150 @default.
- W4242077314 hasRelatedWork W2075404403 @default.
- W4242077314 hasRelatedWork W2118034837 @default.
- W4242077314 hasRelatedWork W2365306427 @default.
- W4242077314 hasRelatedWork W2410054136 @default.
- W4242077314 hasRelatedWork W3158293184 @default.
- W4242077314 hasRelatedWork W4242077314 @default.
- W4242077314 hasRelatedWork W631446107 @default.
- W4242077314 hasVolume "3" @default.
- W4242077314 isParatext "false" @default.
- W4242077314 isRetracted "false" @default.
- W4242077314 workType "article" @default.