Matches in SemOpenAlex for { <https://semopenalex.org/work/W4242118137> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W4242118137 endingPage "1604" @default.
- W4242118137 startingPage "1598" @default.
- W4242118137 abstract "<h3>Objective.</h3> —To determine if the polymerase chain reaction (PCR) can detect bacterial DNA in pediatric middle ear effusions that are sterile by standard cultural methods. <h3>Design.</h3> —Single-center, blinded, comparative study of diagnostic assays. The PCR-based detection systems for<i>Moraxella catarrhalis, Haemophilus influenzae</i>, and<i>Streptococcus pneumoniae</i>were designed and validated using a battery of DNAs obtained from cultured bacteria. Chronic middle ear effusion specimens were collected and comparatively analyzed by culture and the PCR. <h3>Setting.</h3> —Tertiary care pediatric hospital. <h3>Patients.</h3> —A total of 97 middle ear effusions were collected from pediatric outpatients at Children's Hospital of Pittsburgh (Pa) during myringotomy and tube placement for chronic otitis media with effusion (duration >3 months). All patients had failed multiple courses of antimicrobial therapy and were diagnosed by a combination of validated otoscopy and tympanograms. <h3>Main Outcome Measure.</h3> —Differences in the percentage of positive test results between PCR-based assays and culture for<i>M catarrhalis, H influenzae</i>, and<i>S pneumoniae</i>. <h3>Results.</h3> —Of the 97 specimens of otitis media with effusion, 28 (28.9%) tested positive by both culture and PCR for<i>M catarrhalis, H influenzae</i>, or<i>S pneumoniae</i>. An additional 47 specimens (48%) were PCR positive/culture negative for these three bacterial species. Thus, 75 (77.3%) of the 97 specimens tested PCR positive for one or more of the three test organisms. The minimum number of bacterial genomic equivalents present in the average culture-negative ear was estimated to be greater than 10<sup>4</sup>based on dilutional experiments. <h3>Conclusions.</h3> —The PCR-based assay systems can detect the presence of bacterial DNA in a significant percentage of culturally sterile middle ear effusions. While this finding is not proof of an active bacterial infectious process, the large number of bacterial genomic equivalents present in the ears is suggestive of an active process. (<i>JAMA</i>. 1995;273:1598-1604)" @default.
- W4242118137 created "2022-05-12" @default.
- W4242118137 creator A5020711975 @default.
- W4242118137 date "1995-05-24" @default.
- W4242118137 modified "2023-10-03" @default.
- W4242118137 title "Molecular analysis of bacterial pathogens in otitis media with effusion" @default.
- W4242118137 doi "https://doi.org/10.1001/jama.273.20.1598" @default.
- W4242118137 hasPublicationYear "1995" @default.
- W4242118137 type Work @default.
- W4242118137 citedByCount "25" @default.
- W4242118137 countsByYear W42421181372012 @default.
- W4242118137 countsByYear W42421181372019 @default.
- W4242118137 crossrefType "journal-article" @default.
- W4242118137 hasAuthorship W4242118137A5020711975 @default.
- W4242118137 hasConcept C104317684 @default.
- W4242118137 hasConcept C126322002 @default.
- W4242118137 hasConcept C141071460 @default.
- W4242118137 hasConcept C163430725 @default.
- W4242118137 hasConcept C189539697 @default.
- W4242118137 hasConcept C2776408402 @default.
- W4242118137 hasConcept C2776439223 @default.
- W4242118137 hasConcept C2776551000 @default.
- W4242118137 hasConcept C2776925733 @default.
- W4242118137 hasConcept C2777315031 @default.
- W4242118137 hasConcept C2777846895 @default.
- W4242118137 hasConcept C2781253189 @default.
- W4242118137 hasConcept C2910443534 @default.
- W4242118137 hasConcept C49105822 @default.
- W4242118137 hasConcept C501593827 @default.
- W4242118137 hasConcept C523546767 @default.
- W4242118137 hasConcept C54355233 @default.
- W4242118137 hasConcept C55493867 @default.
- W4242118137 hasConcept C71924100 @default.
- W4242118137 hasConcept C86803240 @default.
- W4242118137 hasConcept C89423630 @default.
- W4242118137 hasConcept C90924648 @default.
- W4242118137 hasConceptScore W4242118137C104317684 @default.
- W4242118137 hasConceptScore W4242118137C126322002 @default.
- W4242118137 hasConceptScore W4242118137C141071460 @default.
- W4242118137 hasConceptScore W4242118137C163430725 @default.
- W4242118137 hasConceptScore W4242118137C189539697 @default.
- W4242118137 hasConceptScore W4242118137C2776408402 @default.
- W4242118137 hasConceptScore W4242118137C2776439223 @default.
- W4242118137 hasConceptScore W4242118137C2776551000 @default.
- W4242118137 hasConceptScore W4242118137C2776925733 @default.
- W4242118137 hasConceptScore W4242118137C2777315031 @default.
- W4242118137 hasConceptScore W4242118137C2777846895 @default.
- W4242118137 hasConceptScore W4242118137C2781253189 @default.
- W4242118137 hasConceptScore W4242118137C2910443534 @default.
- W4242118137 hasConceptScore W4242118137C49105822 @default.
- W4242118137 hasConceptScore W4242118137C501593827 @default.
- W4242118137 hasConceptScore W4242118137C523546767 @default.
- W4242118137 hasConceptScore W4242118137C54355233 @default.
- W4242118137 hasConceptScore W4242118137C55493867 @default.
- W4242118137 hasConceptScore W4242118137C71924100 @default.
- W4242118137 hasConceptScore W4242118137C86803240 @default.
- W4242118137 hasConceptScore W4242118137C89423630 @default.
- W4242118137 hasConceptScore W4242118137C90924648 @default.
- W4242118137 hasIssue "20" @default.
- W4242118137 hasLocation W42421181371 @default.
- W4242118137 hasOpenAccess W4242118137 @default.
- W4242118137 hasPrimaryLocation W42421181371 @default.
- W4242118137 hasRelatedWork W2007823809 @default.
- W4242118137 hasRelatedWork W2037402068 @default.
- W4242118137 hasRelatedWork W2103091271 @default.
- W4242118137 hasRelatedWork W2122663840 @default.
- W4242118137 hasRelatedWork W2188917108 @default.
- W4242118137 hasRelatedWork W2361394208 @default.
- W4242118137 hasRelatedWork W3022745209 @default.
- W4242118137 hasRelatedWork W3045869313 @default.
- W4242118137 hasRelatedWork W4242118137 @default.
- W4242118137 hasRelatedWork W4383199037 @default.
- W4242118137 hasVolume "273" @default.
- W4242118137 isParatext "false" @default.
- W4242118137 isRetracted "false" @default.
- W4242118137 workType "article" @default.