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- W4242226932 abstract "The considerable growth of nano‐technologies and nano‐sciences requires the implementation of very well adapted characterization tools and methods to combine both a nanometer resolution and a good preservation of the nano‐objects. This point is absolutely critical when the nanostructures are made of hydrated and organic compounds. Cryo‐electron microscopy (cryo‐TEM) made from vitrified thin films has proved to be a method of choice for characterizing the hierarchy of supramolecular assemblies of proteins and hydroxy fatty acids with maximum resolution and minimal damage. We aim here to present a selection of applications of cryo‐TEM showing the characterization of dispersions of organic or hybrid organic/inorganic nanoparticles with a large range of sizes, shapes and composition (synthetic polymers, polysaccharides, proteins, lipids). In particular, we will detail how cryo‐TEM helped to elucidate the supramolecular self‐assembling of fatty acids through a multiscale approach. The unique benefits as well as the limitations of cryo‐TEM will be discussed and compared with conventional SEM and TEM techniques as well as with liquid AFM." @default.
- W4242226932 created "2022-05-12" @default.
- W4242226932 creator A5053991344 @default.
- W4242226932 date "2016-12-20" @default.
- W4242226932 modified "2023-09-26" @default.
- W4242226932 title "Cryo‐electron microscopy from vitreous thin films in complement to <scp>AFM</scp> for understanding the multiscale supramolecular assemblies: a few examples" @default.
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- W4242226932 doi "https://doi.org/10.1002/9783527808465.emc2016.6984" @default.
- W4242226932 hasPublicationYear "2016" @default.
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