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• W4242256325 endingPage "2088" @default.
• W4242256325 startingPage "2080" @default.
• W4242256325 abstract "At least six mRNAs are made from the Drosophila melanogaster act5C gene. We investigated the structures of these RNAs in detail and determined that they are heterogeneous at both their 5' and 3' ends. At the 5' end there were two nonhomologous leader exons which were alternately spliced to the remainder of the gene. These leader exons mapped to 1.7 and 0.7 kilobases, respectively, upstream of a common splice acceptor site which was eight base pairs 5' to the translation initiator AUG. Exon 1 is 147 bases in length, while exon 2 is 111 bases. A consensus TATA sequence was found roughly 30 base pairs upstream from exon 1, but none was found in the analogous position upstream of exon 2. The transcript length diversity arose principally from the use of three polyadenylation sites. This gave rise to RNA molecules with 3'-untranslated regions of roughly 375, 655, and 945 base pairs. With two start sites and three termination sites, this gene has the potential to produce six different transcripts. All six possible transcripts were present in whole fly mRNA. Transcripts containing the two different leader exons were found in roughly the same relative quantities through development. In contrast, the various 3' ends were differentially represented through development." @default.
• W4242256325 created "2022-05-12" @default.
• W4242256325 creator A5041209392 @default.
• W4242256325 creator A5065805381 @default.
• W4242256325 date "1986-06-01" @default.
• W4242256325 modified "2023-09-25" @default.
• W4242256325 title "The <i>Drosophila melanogaster</i> Actin 5C Gene Uses Two Transcription Initiation Sites and Three Polyadenylation Sites To Express Multiple mRNA Species" @default.
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• W4242256325 doi "https://doi.org/10.1128/mcb.6.6.2080-2088.1986" @default.
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