Matches in SemOpenAlex for { <https://semopenalex.org/work/W4242625549> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W4242625549 endingPage "3299" @default.
- W4242625549 startingPage "3289" @default.
- W4242625549 abstract "Pyrosequencing is a four-enzyme bioluminometric DNA sequencing technique based on a DNA sequencing by synthesis principle. Currently, the technique is limited to analysis of short DNA sequences exemplified by single-nucleotide polymorphism analysis. In order to expand the field for pyrosequencing, the read length needs to be improved and efforts have been made to purify reaction components as well as add single-stranded DNA-binding protein (SSB) to the pyrosequencing reaction. In this study, we have performed a systematic effort to analyze the effects of SSB by comparing the pyrosequencing result of 103 independent complementary DNA (cDNA) clones. More detailed information about the cause of low quality sequences on templates with different characteristics was achieved by thorough analysis of the pyrograms. Also, real-time biosensor analysis was performed on individual cDNA clones for investigation of primer annealing and SSB binding on these templates. Results from these studies indicate that templates with high performance in pyrosequencing without SSB possess efficient primer annealing and low SSB affinity. Alternative strategies to improve the performance in pyrosequencing by increasing the primer-annealing efficiency have also been evaluated." @default.
- W4242625549 created "2022-05-12" @default.
- W4242625549 creator A5006305357 @default.
- W4242625549 creator A5020047304 @default.
- W4242625549 creator A5044920044 @default.
- W4242625549 creator A5051573418 @default.
- W4242625549 creator A5054656710 @default.
- W4242625549 date "2002-10-01" @default.
- W4242625549 modified "2023-09-30" @default.
- W4242625549 doi "https://doi.org/10.1002/1522-2683(200210)23:19<3289::aid-elps3289>3.0.co;2-" @default.
- W4242625549 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12373756" @default.
- W4242625549 hasPublicationYear "2002" @default.
- W4242625549 type Work @default.
- W4242625549 citedByCount "16" @default.
- W4242625549 countsByYear W42426255492016 @default.
- W4242625549 crossrefType "journal-article" @default.
- W4242625549 hasAuthorship W4242625549A5006305357 @default.
- W4242625549 hasAuthorship W4242625549A5020047304 @default.
- W4242625549 hasAuthorship W4242625549A5044920044 @default.
- W4242625549 hasAuthorship W4242625549A5051573418 @default.
- W4242625549 hasAuthorship W4242625549A5054656710 @default.
- W4242625549 hasConcept C104317684 @default.
- W4242625549 hasConcept C125009961 @default.
- W4242625549 hasConcept C153911025 @default.
- W4242625549 hasConcept C178790620 @default.
- W4242625549 hasConcept C185592680 @default.
- W4242625549 hasConcept C187882448 @default.
- W4242625549 hasConcept C199360897 @default.
- W4242625549 hasConcept C2777563447 @default.
- W4242625549 hasConcept C41008148 @default.
- W4242625549 hasConcept C51679486 @default.
- W4242625549 hasConcept C54355233 @default.
- W4242625549 hasConcept C552990157 @default.
- W4242625549 hasConcept C70721500 @default.
- W4242625549 hasConcept C82714645 @default.
- W4242625549 hasConcept C86803240 @default.
- W4242625549 hasConceptScore W4242625549C104317684 @default.
- W4242625549 hasConceptScore W4242625549C125009961 @default.
- W4242625549 hasConceptScore W4242625549C153911025 @default.
- W4242625549 hasConceptScore W4242625549C178790620 @default.
- W4242625549 hasConceptScore W4242625549C185592680 @default.
- W4242625549 hasConceptScore W4242625549C187882448 @default.
- W4242625549 hasConceptScore W4242625549C199360897 @default.
- W4242625549 hasConceptScore W4242625549C2777563447 @default.
- W4242625549 hasConceptScore W4242625549C41008148 @default.
- W4242625549 hasConceptScore W4242625549C51679486 @default.
- W4242625549 hasConceptScore W4242625549C54355233 @default.
- W4242625549 hasConceptScore W4242625549C552990157 @default.
- W4242625549 hasConceptScore W4242625549C70721500 @default.
- W4242625549 hasConceptScore W4242625549C82714645 @default.
- W4242625549 hasConceptScore W4242625549C86803240 @default.
- W4242625549 hasIssue "19" @default.
- W4242625549 hasLocation W42426255491 @default.
- W4242625549 hasLocation W42426255492 @default.
- W4242625549 hasOpenAccess W4242625549 @default.
- W4242625549 hasPrimaryLocation W42426255491 @default.
- W4242625549 hasRelatedWork W2013529626 @default.
- W4242625549 hasRelatedWork W2019195734 @default.
- W4242625549 hasRelatedWork W2088017464 @default.
- W4242625549 hasRelatedWork W2165308292 @default.
- W4242625549 hasRelatedWork W2359698256 @default.
- W4242625549 hasRelatedWork W2359741505 @default.
- W4242625549 hasRelatedWork W2367078647 @default.
- W4242625549 hasRelatedWork W2367445045 @default.
- W4242625549 hasRelatedWork W2375757446 @default.
- W4242625549 hasRelatedWork W2398349715 @default.
- W4242625549 hasVolume "23" @default.
- W4242625549 isParatext "false" @default.
- W4242625549 isRetracted "false" @default.
- W4242625549 workType "article" @default.