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- W4242646598 abstract "A commercially available diluting buffer (ADB) was used to customize prediluted immunohistochemical kits from the less expensive concentrated form. These kits were used for a broad spectrum of antibodies in our neuropathology laboratory. The ADB allows the conversion of existing immunohistochemical (IHC) stains that employ concentrated regents into a complete prediluted system, without the necessity for changing existing dilutions and incubation times. This commercial buffer enables the user to predilute and store in bulk not only primary antibodies (up to 1 yr at 4°C) but blocking serum, biotinylated antiserum, and enzyme conjugated antiserum (up to 2 mo at 4°C). Furthermore, a color coding kit for visual identification of the various reagents is available to complement this system. An in-house prediluted system enables the technologist to reduce the initial preparation time, and is far more cost effective than prediluted kits and primary antibodies. (The J Histotechnol 18:41, 1995)" @default.
- W4242646598 created "2022-05-12" @default.
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- W4242646598 date "1995-03-01" @default.
- W4242646598 modified "2023-09-26" @default.
- W4242646598 title "Streamlining the Immunopathology Laboratory with Commercial Antibody Diluting Buffer" @default.
- W4242646598 doi "https://doi.org/10.1179/014788895794710453" @default.
- W4242646598 hasPublicationYear "1995" @default.
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