FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4242653178> ?p ?o ?g. }

• W4242653178 abstract "Abstract Introduction: Spinal cord injury (SCI) is a neurological, medically incurable disorder. Human pluripotent stem cells (hPSCs) have the potential to generate neural stem/progenitor cells (NS/PCs) which hold promise in therapy for SCI by transplantation. In our study, we aimed to establish a chemically defined culture system by using serum-free medium and ascorbic acid (AA) to generation and expansion of long-term self-renewing neuroepithelial-like stem cells (lt-NES cells) differentiated from hPSCs effectively and stably. Methods: We induce hESC/iPSC to neurospheres by using a newly established induction system in vitro in our study. And lt-NES cells derived from hESCs/iPSCs-neurospheres using two induction systems, including conventional N2 medium with gelatin-coated (coated) and N2+AA medium without pre-coated (AA) were characterized by reverse transcription-polymerase chain reaction (RT-PCR) analysis and immunocytochemistry staining. Subsequently, lt-NES cells were induced to neurons and the microelectrode array (MEA) recording system was used to evaluate the functionality of neurons differentiated from lt-NES cells. Moreover, the mechanism of AA-induced lt-NES cells was explored through RNA-seq and the use of inhibitors. Results: HESCs/iPSCs were efficiently induced to neurospheres by using a newly established induction system in vitro. And lt-NES cells derived from hESCs/iPSCs-neurospheres using two induction system (coated vs AA) both expressed neural pluripotency-associated genes PAX6, NESTIN, SOX1, SOX2. After long-term cultivation, we found that they both can maintain the long-term expansion for more than a dozen generations while maintaining neuropluripotency. Moreover, the lt-NES cells retain the ability to differentiate into general functional neurons that highly express β-tubulin. We also demonstrated that AA promotes the generation and long-term expansion of lt-NES cells by promoting collagen synthesis via the MEK-ERK1/2 pathways. Conclusions: Taken together, this new chemically defined culture system is stable and effective to generate and culture the lt-NES cells induced by hESCs/iPSCs using serum-free medium combined with ascorbic acid (AA). The lt-NES cells under this culture system can maintain the long-term expansion and neural pluripotency, with the potential to differentiate into functional neurons. Keywords: Spinal cord injury, Neurospheres, Ascorbic acid, lt-NES cells, Human pluripotent stem cells." @default.
• W4242653178 created "2022-05-12" @default.
• W4242653178 creator A5000663583 @default.
• W4242653178 creator A5006557162 @default.
• W4242653178 creator A5006838194 @default.
• W4242653178 creator A5016453378 @default.
• W4242653178 creator A5033747658 @default.
• W4242653178 creator A5041981383 @default.
• W4242653178 creator A5042619808 @default.
• W4242653178 creator A5049321053 @default.
• W4242653178 creator A5053151070 @default.
• W4242653178 creator A5058582642 @default.
• W4242653178 creator A5065641837 @default.
• W4242653178 creator A5074846688 @default.
• W4242653178 creator A5086519113 @default.
• W4242653178 date "2020-09-24" @default.
• W4242653178 modified "2023-09-26" @default.
• W4242653178 title "Ascorbic Acid Can Promote the Generation and Expansion of Neuroepithelial-Like Stem Cells Derived from HiPS/ES Cells Under Chemically Defined Conditions Through Promoting Collagen Synthesis" @default.
• W4242653178 doi "https://doi.org/10.21203/rs.3.rs-79357/v1" @default.
• W4242653178 hasPublicationYear "2020" @default.
• W4242653178 type Work @default.
• W4242653178 citedByCount "0" @default.
• W4242653178 crossrefType "posted-content" @default.
• W4242653178 hasAuthorship W4242653178A5000663583 @default.
• W4242653178 hasAuthorship W4242653178A5006557162 @default.
• W4242653178 hasAuthorship W4242653178A5006838194 @default.
• W4242653178 hasAuthorship W4242653178A5016453378 @default.
• W4242653178 hasAuthorship W4242653178A5033747658 @default.
• W4242653178 hasAuthorship W4242653178A5041981383 @default.
• W4242653178 hasAuthorship W4242653178A5042619808 @default.
• W4242653178 hasAuthorship W4242653178A5049321053 @default.
• W4242653178 hasAuthorship W4242653178A5053151070 @default.
• W4242653178 hasAuthorship W4242653178A5058582642 @default.
• W4242653178 hasAuthorship W4242653178A5065641837 @default.
• W4242653178 hasAuthorship W4242653178A5074846688 @default.
• W4242653178 hasAuthorship W4242653178A5086519113 @default.
• W4242653178 hasBestOaLocation W42426531781 @default.
• W4242653178 hasConcept C104317684 @default.
• W4242653178 hasConcept C107459253 @default.
• W4242653178 hasConcept C136834591 @default.
• W4242653178 hasConcept C145103041 @default.
• W4242653178 hasConcept C153911025 @default.
• W4242653178 hasConcept C162746124 @default.
• W4242653178 hasConcept C185592680 @default.
• W4242653178 hasConcept C200707615 @default.
• W4242653178 hasConcept C20875687 @default.
• W4242653178 hasConcept C2777738006 @default.
• W4242653178 hasConcept C2777933648 @default.
• W4242653178 hasConcept C28328180 @default.
• W4242653178 hasConcept C2986274086 @default.
• W4242653178 hasConcept C31903555 @default.
• W4242653178 hasConcept C55493867 @default.
• W4242653178 hasConcept C86803240 @default.
• W4242653178 hasConcept C95444343 @default.
• W4242653178 hasConceptScore W4242653178C104317684 @default.
• W4242653178 hasConceptScore W4242653178C107459253 @default.
• W4242653178 hasConceptScore W4242653178C136834591 @default.
• W4242653178 hasConceptScore W4242653178C145103041 @default.
• W4242653178 hasConceptScore W4242653178C153911025 @default.
• W4242653178 hasConceptScore W4242653178C162746124 @default.
• W4242653178 hasConceptScore W4242653178C185592680 @default.
• W4242653178 hasConceptScore W4242653178C200707615 @default.
• W4242653178 hasConceptScore W4242653178C20875687 @default.
• W4242653178 hasConceptScore W4242653178C2777738006 @default.
• W4242653178 hasConceptScore W4242653178C2777933648 @default.
• W4242653178 hasConceptScore W4242653178C28328180 @default.
• W4242653178 hasConceptScore W4242653178C2986274086 @default.
• W4242653178 hasConceptScore W4242653178C31903555 @default.
• W4242653178 hasConceptScore W4242653178C55493867 @default.
• W4242653178 hasConceptScore W4242653178C86803240 @default.
• W4242653178 hasConceptScore W4242653178C95444343 @default.
• W4242653178 hasLocation W42426531781 @default.
• W4242653178 hasLocation W42426531782 @default.
• W4242653178 hasOpenAccess W4242653178 @default.
• W4242653178 hasPrimaryLocation W42426531781 @default.
• W4242653178 hasRelatedWork W13257616 @default.
• W4242653178 hasRelatedWork W13324867 @default.
• W4242653178 hasRelatedWork W17797412 @default.
• W4242653178 hasRelatedWork W18068426 @default.
• W4242653178 hasRelatedWork W3018495 @default.
• W4242653178 hasRelatedWork W5681630 @default.
• W4242653178 hasRelatedWork W6678017 @default.
• W4242653178 hasRelatedWork W8713738 @default.
• W4242653178 hasRelatedWork W953999 @default.
• W4242653178 hasRelatedWork W9564727 @default.
• W4242653178 isParatext "false" @default.
• W4242653178 isRetracted "false" @default.
• W4242653178 workType "article" @default.