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- W4242799468 abstract "Abstract Background SARS-CoV-2 has been detected not only in respiratory secretions, but also in stool collections. Here were sought to identify SARS-CoV-2 by enrichment NGS from fecal samples, and to utilize whole genome analysis to characterize SARS-CoV-2 mutational variations in COVID-19 patients. Results Study participants underwent testing for SARS-CoV-2 from fecal samples by whole genome enrichment NGS (n = 14), and RT-PCR nasopharyngeal swab analysis (n = 12). The concordance of SARS-CoV-2 detection by enrichment NGS from stools with RT-PCR nasopharyngeal analysis was 100%. Unique variants were identified in four patients, with a total of 33 different mutations among those in which SARS-CoV-2 was detected by whole genome enrichment NGS. Conclusion These results highlight the potential viability of SARS-CoV-2 in feces, its ongoing mutational accumulation, and its possible role in fecal-oral transmission. This study also elucidates the advantages of SARS-CoV-2 enrichment NGS, which may be a key methodology to document complete viral eradication." @default.
- W4242799468 created "2022-05-12" @default.
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- W4242799468 date "2021-01-15" @default.
- W4242799468 modified "2023-09-25" @default.
- W4242799468 title "Detection of SARS-CoV-2 from Patient Fecal Samples by Whole Genome Sequencing" @default.
- W4242799468 doi "https://doi.org/10.21203/rs.3.rs-48863/v3" @default.
- W4242799468 hasPublicationYear "2021" @default.
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