FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4242807496> ?p ?o ?g. }

• W4242807496 endingPage "1350" @default.
• W4242807496 startingPage "1350" @default.
• W4242807496 abstract "To the Editors:The recent article by Kumar et al. (1) in the September 1, 2006 issue of Clinical Cancer Research challenged the presence of salivary mRNA. They concluded that the source of the published RNA signals detected in human saliva is from genomic DNA contamination. This letter provides a different perspective on this subject and rebuts the investigators' conclusion.The major discrepancy is the claim by these investigators (1) that they have reproduced the experimental conditions of the Li et al. article (2) without detecting mRNA. A careful comparison of the two articles shows that there are significant differences. First and foremost, Kumar et al. used RNAlater (Ambion Inc., Austin, TX) to stabilize salivary RNA, whereas Li et al. used Superase-In (Ambion, Inc., Austin, TX), a protein-based inhibitor efficient in inhibiting different types of nucleases, including RNase A, B, C, 1, and T1. Other than inhibiting nucleases, it has no additional effect in saliva. RNAlater, on the other hand, does a lot more than just preserving RNA. RNAlater increases cellular membrane permeability and its high salt content (ammonium sulfate) changes intracellular salt concentration, leading to leakage of cellular components, including genomic DNA. We have recently compared a number of RNA stabilizers and found RNAlater to be the least suitable, even worse than without any stabilizer (3).Second, the methods used by the Kumar et al. cannot isolate RNA from saliva, not even from the cellular pellet. The failure to isolate RNA from the saliva cell pellet should signal concerns as it is enriched in epithelial cells and leukocytes, which often serve as an internal positive control for RNA isolation. The investigators used a different RNA isolation procedure than the one published by Li et al. (2). The failure to isolate RNA from the saliva cellular pellet suggests the inability of their procedure to harvest RNA from saliva.The scientific credibility of the salivary transcriptome is increasing, ranging from recent studies on the characterization of salivary RNA (4), to assessing salivary RNA integrity by cDNA library analysis (5), to identifying and commercializing the most effective stabilizer, RNA Protect Saliva (QIAGEN, Valencia, CA) for research and eventual clinical applications (3). The salivary cDNA library analysis conclusively showed that none of the detected mRNA in saliva is from a pseudogene of contaminating genomic origin, independently disputing DNA as a source of templates giving rise to false-positive RNA signals (5). Other research groups, particularly from forensic sciences, are focusing on multiplex mRNA profiling for the identification of body fluids, including saliva (6–9). The most recent study from Seugnet et al. compellingly shows the value of the amylase mRNA in human saliva as a highly discriminatory biomarker for sleep drive (10)." @default.
• W4242807496 created "2022-05-12" @default.
• W4242807496 creator A5087543035 @default.
• W4242807496 date "2007-02-15" @default.
• W4242807496 modified "2023-09-26" @default.
• W4242807496 title "Salivary Transcriptome" @default.
• W4242807496 cites W1971991351 @default.
• W4242807496 cites W2019223199 @default.
• W4242807496 cites W2027363946 @default.
• W4242807496 cites W2031795328 @default.
• W4242807496 cites W2077133630 @default.
• W4242807496 cites W2106295250 @default.
• W4242807496 cites W2113272687 @default.
• W4242807496 cites W2115757940 @default.
• W4242807496 cites W2124262378 @default.
• W4242807496 cites W2127839469 @default.
• W4242807496 doi "https://doi.org/10.1158/1078-0432.ccr-06-2227" @default.
• W4242807496 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17317847" @default.
• W4242807496 hasPublicationYear "2007" @default.
• W4242807496 type Work @default.
• W4242807496 citedByCount "8" @default.
• W4242807496 countsByYear W42428074962012 @default.
• W4242807496 countsByYear W42428074962013 @default.
• W4242807496 countsByYear W42428074962015 @default.
• W4242807496 countsByYear W42428074962021 @default.
• W4242807496 crossrefType "journal-article" @default.
• W4242807496 hasAuthorship W4242807496A5087543035 @default.
• W4242807496 hasBestOaLocation W42428074961 @default.
• W4242807496 hasConcept C104317684 @default.
• W4242807496 hasConcept C150194340 @default.
• W4242807496 hasConcept C153911025 @default.
• W4242807496 hasConcept C162317418 @default.
• W4242807496 hasConcept C185592680 @default.
• W4242807496 hasConcept C504460877 @default.
• W4242807496 hasConcept C552990157 @default.
• W4242807496 hasConcept C55493867 @default.
• W4242807496 hasConcept C67705224 @default.
• W4242807496 hasConcept C86803240 @default.
• W4242807496 hasConceptScore W4242807496C104317684 @default.
• W4242807496 hasConceptScore W4242807496C150194340 @default.
• W4242807496 hasConceptScore W4242807496C153911025 @default.
• W4242807496 hasConceptScore W4242807496C162317418 @default.
• W4242807496 hasConceptScore W4242807496C185592680 @default.
• W4242807496 hasConceptScore W4242807496C504460877 @default.
• W4242807496 hasConceptScore W4242807496C552990157 @default.
• W4242807496 hasConceptScore W4242807496C55493867 @default.
• W4242807496 hasConceptScore W4242807496C67705224 @default.
• W4242807496 hasConceptScore W4242807496C86803240 @default.
• W4242807496 hasIssue "4" @default.
• W4242807496 hasLocation W42428074961 @default.
• W4242807496 hasLocation W42428074962 @default.
• W4242807496 hasOpenAccess W4242807496 @default.
• W4242807496 hasPrimaryLocation W42428074961 @default.
• W4242807496 hasRelatedWork W1971124177 @default.
• W4242807496 hasRelatedWork W1976736675 @default.
• W4242807496 hasRelatedWork W2002473487 @default.
• W4242807496 hasRelatedWork W2009966535 @default.
• W4242807496 hasRelatedWork W2102579905 @default.
• W4242807496 hasRelatedWork W2513916811 @default.
• W4242807496 hasRelatedWork W3089353767 @default.
• W4242807496 hasRelatedWork W3097825150 @default.
• W4242807496 hasRelatedWork W3146618441 @default.
• W4242807496 hasRelatedWork W4247273247 @default.
• W4242807496 hasVolume "13" @default.
• W4242807496 isParatext "false" @default.
• W4242807496 isRetracted "false" @default.
• W4242807496 workType "article" @default.