Matches in SemOpenAlex for { <https://semopenalex.org/work/W4242912161> ?p ?o ?g. }
Showing items 1 to 51 of
51
with 100 items per page.
- W4242912161 endingPage "2" @default.
- W4242912161 startingPage "1" @default.
- W4242912161 abstract "During both human and animal vaccine development phases, animal testing is necessary to demonstrate vaccine efficacy. Since the number of antigen candidates for testing is usually large when developing a potential vaccine, it is too costly, time consuming and would involve higher risks to carry out selection using in vivo models. The currently available in vitro assays that measure immunogenicity do not adequately reproduce the in vivo state and this is especially true for vaccine research in livestock species. With this in mind, we have developed a bovine monocyte derived dendritic cell (MODC)s based assay to prime CD4 and CD8 lymphocytes in order to investigate vaccine immunogenicity in vitro. MODCs were generated, pulsed with diphtheria toxoid (DT) and co-cultured with lymphocytes for priming. Immunogenicity was measured through antigen recall when antigen pulsed MODC were re-introduced to the co-culture and proliferation of CD4 and CD8 positive lymphocytes were quantified using expressed Ki-67. Having developed the protocol for the assay, we then employed two licenced vaccines against blue tongue virus and rabies virus to validate the assay. Our results show the ability of the assay to satisfactorily measure immunogenicity in cattle. The assay could be used to identify antigens that induce CD4 and CD8 T cell responses prior to embarking on in vivo experiments and can also be used for the quality control of established vaccines in vaccine production facilities as a supplement for in vivo experiments." @default.
- W4242912161 created "2022-05-12" @default.
- W4242912161 date "2015-05-01" @default.
- W4242912161 modified "2023-09-27" @default.
- W4242912161 title "Editorial" @default.
- W4242912161 doi "https://doi.org/10.1016/j.semcdb.2015.05.010" @default.
- W4242912161 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26094160" @default.
- W4242912161 hasPublicationYear "2015" @default.
- W4242912161 type Work @default.
- W4242912161 citedByCount "0" @default.
- W4242912161 crossrefType "journal-article" @default.
- W4242912161 hasConcept C147483822 @default.
- W4242912161 hasConcept C150903083 @default.
- W4242912161 hasConcept C159047783 @default.
- W4242912161 hasConcept C167672396 @default.
- W4242912161 hasConcept C203014093 @default.
- W4242912161 hasConcept C207001950 @default.
- W4242912161 hasConcept C2522874641 @default.
- W4242912161 hasConcept C2777961786 @default.
- W4242912161 hasConcept C2780868878 @default.
- W4242912161 hasConcept C86803240 @default.
- W4242912161 hasConceptScore W4242912161C147483822 @default.
- W4242912161 hasConceptScore W4242912161C150903083 @default.
- W4242912161 hasConceptScore W4242912161C159047783 @default.
- W4242912161 hasConceptScore W4242912161C167672396 @default.
- W4242912161 hasConceptScore W4242912161C203014093 @default.
- W4242912161 hasConceptScore W4242912161C207001950 @default.
- W4242912161 hasConceptScore W4242912161C2522874641 @default.
- W4242912161 hasConceptScore W4242912161C2777961786 @default.
- W4242912161 hasConceptScore W4242912161C2780868878 @default.
- W4242912161 hasConceptScore W4242912161C86803240 @default.
- W4242912161 hasLocation W42429121611 @default.
- W4242912161 hasLocation W42429121612 @default.
- W4242912161 hasOpenAccess W4242912161 @default.
- W4242912161 hasPrimaryLocation W42429121611 @default.
- W4242912161 hasRelatedWork W2038220108 @default.
- W4242912161 hasRelatedWork W2316406995 @default.
- W4242912161 hasRelatedWork W2349241371 @default.
- W4242912161 hasRelatedWork W2361192876 @default.
- W4242912161 hasRelatedWork W2379987678 @default.
- W4242912161 hasRelatedWork W2382453547 @default.
- W4242912161 hasRelatedWork W2503218969 @default.
- W4242912161 hasRelatedWork W3144031950 @default.
- W4242912161 hasRelatedWork W3149370582 @default.
- W4242912161 hasRelatedWork W4200459600 @default.
- W4242912161 hasVolume "41" @default.
- W4242912161 isParatext "false" @default.
- W4242912161 isRetracted "false" @default.
- W4242912161 workType "editorial" @default.