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- W4242922021 abstract "Abstract The aim of this study was to examine four strains of two yeast species in relation to their capability for assimilating alkanes in the presence of heavy metals (HMs). The four strains tested were Candida pseudoglaebosa ENCB‐7 and Kodamaea ohmeri ENCB‐8R, ENCB‐23, and ENCB‐VIK. Determination was made of the expression of CYP52 genes involved in alkane hydroxylation. When exposed to Cu 2+ , Zn 2+ , Pb 2+ , Cd 2+ , and As 3+ at pH 3 and 5, all four strains could assimilate several n‐ alkanes having at least six carbon atoms. The three K. ohmeri strains could also utilize branched alkanes, cycloalkanes, and n ‐octanol as sole carbon sources. Kinetic assays demonstrated greater biomass production and specific growth of the yeasts exposed to long‐chain n ‐alkanes. Fragments of paralogous CYP52 genes of C. pseudoglaebosa ENCB‐7 and K. ohmeri ENCB‐23 were amplified, sequenced, and phylogenetically evaluated. Reverse‐transcription polymerase chain reaction revealed that n ‐nonane and n ‐decane induced to CpCYP52‐G3 , CpCYP52‐G9 , and CpCYP52‐G10. KoCYP52‐G3 was induced with n ‐decane and n ‐octanol. Also, CpCYP52‐G3 and CpCYP52‐G9 were induced by glucose. In conclusion, C. pseudoglaebosa and K. ohmeri were able to degrade several alkanes in the presence of HMs and under acidic conditions. These yeasts harbor paralogous alkane‐induced CYP52 genes, which display different profiles of transcriptional expression." @default.
- W4242922021 created "2022-05-12" @default.
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- W4242922021 date "2019-08-01" @default.
- W4242922021 modified "2023-10-18" @default.
- W4242922021 title "<i>Candida pseudoglaebosa</i> and <i>Kodamaea ohmeri</i> are capable of degrading alkanes in the presence of heavy metals" @default.
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- W4242922021 doi "https://doi.org/10.1002/jobm.201900027" @default.
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