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- W4242958324 abstract "Abstract Mithilfe des UAG‐Codons wurde ein Pyrrolysin‐Analogon mit terminaler Alkinfunktion ortsspezifisch in rekombinantes Calmodulin (CaM) eingebaut. Das resultierende Protein wurde über eine Kupfer(I)‐katalysierte Klick‐Reaktion mit der Azidgruppe eines Farbstoffs verknüpft. Die anschließende Anwendung einer orthogonalen Cystein‐Markierungsmethode ergab mit zwei unterschiedlichen Fluorophoren markiertes CaM für FRET‐Studien. magnified image" @default.
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- W4242958324 date "2009-02-10" @default.
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- W4242958324 title "A Pyrrolysine Analogue for Protein Click Chemistry" @default.
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- W4242958324 doi "https://doi.org/10.1002/ange.200805420" @default.
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