Matches in SemOpenAlex for { <https://semopenalex.org/work/W4243100448> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W4243100448 endingPage "677" @default.
- W4243100448 startingPage "677" @default.
- W4243100448 abstract "The enzyme prostaglandin endoperoxide H synthase (PGHS) has a pivotal role in the prostanoid biosynthetic pathway because it catalyses the formation of prostaglandin H2 (PGH2), the common precursor of prostanoids. Two PGHS isoforms have been reported, PGHS-1 and PGHS-2, which have 61% identity (at the amino acid level) and 73% similarity (at the nucleotide level) between the two human enzymes. Transcription of the PGHS-1 gene leads to the formation of two transcripts (2.8 and 5.1 kb); two transcripts of 2.8 and 4.5 kb are produced from the PGHS-2 gene. By Northern blot analysis with the entire coding region of human PGHS-1, 2.8 and 5.1 kb transcripts as well as a novel 4.5 kb transcript were detected in the human megakaryoblastic cell line MEG-01. We designed a strategy to characterize the 4.5 kb PGHS transcript. Probes specific for each PGHS-1 and PGHS-2 were designed on the basis of the 3ʹ untranslated region (3ʹ UTR), where no similarity is present. The 4.5 kb transcript was detected only with the PGHS-1-specific 3ʹ UTR probes and not with the PGHS-2-specific 3ʹ UTR probe. To investigate the origin of the 4.5 kb PGHS-1 transcript, the remaining 947 bp of the 5.1 kb PGHS-1 transcript was generated by 3ʹ rapid amplification of cDNA ends (3ʹ RACE) and sequenced. A non-canonical polyadenylation signal (AAGAAA) located upstream of a potential cleavage site (CA) was found and could generate the 4.5 kb PGHS-1 transcript. Analysis of the sequence also produced several possible G/U-rich elements downstream of the potential cleavage site. An RNA dot-blot with 50 different human tissues was probed with the 4.5 and 5.1 kb PGHS-1-specific probes. A signal for the 4.5 kb PGHS-1 transcript was detected in the bladder and appendix. Signals of lower intensity were detected in the colon, bone marrow, small intestine, uterus, prostate, peripheral leucocyte, lymph node and stomach. In conclusion, our results suggest that the cell line MEG-01, the bladder and the appendix contain a new PGHS-1 transcript of 4.5 kb that can be produced from the PGHS-1 gene and we provide a better strategy for distinguishing PGHS-1 transcripts from PGHS-2." @default.
- W4243100448 created "2022-05-12" @default.
- W4243100448 creator A5006698956 @default.
- W4243100448 creator A5053225327 @default.
- W4243100448 date "1999-12-15" @default.
- W4243100448 modified "2023-09-23" @default.
- W4243100448 title "Characterization of a novel transcript of prostaglandin endoperoxide H synthase 1 with a tissue-specific profile of expression" @default.
- W4243100448 doi "https://doi.org/10.1042/0264-6021:3440677" @default.
- W4243100448 hasPublicationYear "1999" @default.
- W4243100448 type Work @default.
- W4243100448 citedByCount "7" @default.
- W4243100448 countsByYear W42431004482014 @default.
- W4243100448 crossrefType "journal-article" @default.
- W4243100448 hasAuthorship W4243100448A5006698956 @default.
- W4243100448 hasAuthorship W4243100448A5053225327 @default.
- W4243100448 hasBestOaLocation W42431004482 @default.
- W4243100448 hasConcept C104317684 @default.
- W4243100448 hasConcept C105580179 @default.
- W4243100448 hasConcept C138885662 @default.
- W4243100448 hasConcept C142575336 @default.
- W4243100448 hasConcept C150194340 @default.
- W4243100448 hasConcept C153911025 @default.
- W4243100448 hasConcept C177707886 @default.
- W4243100448 hasConcept C179926584 @default.
- W4243100448 hasConcept C181199279 @default.
- W4243100448 hasConcept C187882448 @default.
- W4243100448 hasConcept C2778078955 @default.
- W4243100448 hasConcept C2780978440 @default.
- W4243100448 hasConcept C41895202 @default.
- W4243100448 hasConcept C53345823 @default.
- W4243100448 hasConcept C54355233 @default.
- W4243100448 hasConcept C55493867 @default.
- W4243100448 hasConcept C86803240 @default.
- W4243100448 hasConcept C89604277 @default.
- W4243100448 hasConcept C91779695 @default.
- W4243100448 hasConceptScore W4243100448C104317684 @default.
- W4243100448 hasConceptScore W4243100448C105580179 @default.
- W4243100448 hasConceptScore W4243100448C138885662 @default.
- W4243100448 hasConceptScore W4243100448C142575336 @default.
- W4243100448 hasConceptScore W4243100448C150194340 @default.
- W4243100448 hasConceptScore W4243100448C153911025 @default.
- W4243100448 hasConceptScore W4243100448C177707886 @default.
- W4243100448 hasConceptScore W4243100448C179926584 @default.
- W4243100448 hasConceptScore W4243100448C181199279 @default.
- W4243100448 hasConceptScore W4243100448C187882448 @default.
- W4243100448 hasConceptScore W4243100448C2778078955 @default.
- W4243100448 hasConceptScore W4243100448C2780978440 @default.
- W4243100448 hasConceptScore W4243100448C41895202 @default.
- W4243100448 hasConceptScore W4243100448C53345823 @default.
- W4243100448 hasConceptScore W4243100448C54355233 @default.
- W4243100448 hasConceptScore W4243100448C55493867 @default.
- W4243100448 hasConceptScore W4243100448C86803240 @default.
- W4243100448 hasConceptScore W4243100448C89604277 @default.
- W4243100448 hasConceptScore W4243100448C91779695 @default.
- W4243100448 hasIssue "3" @default.
- W4243100448 hasLocation W42431004481 @default.
- W4243100448 hasLocation W42431004482 @default.
- W4243100448 hasOpenAccess W4243100448 @default.
- W4243100448 hasPrimaryLocation W42431004481 @default.
- W4243100448 hasRelatedWork W106503236 @default.
- W4243100448 hasRelatedWork W1995193834 @default.
- W4243100448 hasRelatedWork W1999653867 @default.
- W4243100448 hasRelatedWork W2066484244 @default.
- W4243100448 hasRelatedWork W2074050642 @default.
- W4243100448 hasRelatedWork W2083535977 @default.
- W4243100448 hasRelatedWork W2095293288 @default.
- W4243100448 hasRelatedWork W2114525272 @default.
- W4243100448 hasRelatedWork W2344678176 @default.
- W4243100448 hasRelatedWork W251730109 @default.
- W4243100448 hasVolume "344" @default.
- W4243100448 isParatext "false" @default.
- W4243100448 isRetracted "false" @default.
- W4243100448 workType "article" @default.