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- W4243363525 abstract "The production of bacterial magnetic particles (BMPs) by recombinant Magnetospirillum magneticum AMB-1 harboring the plasmid pKML was enhanced in pH-regulated fed-batch culture. The addition of fresh nutrients was feedback-controlled as a function of the pH of the culture. The yield of BMPs was optimized by adjusting the rate of ferric iron addition. Feeding ferric quinate at 15.4 microg/min resulted ina BMP yield of 7.5 mg/l, which is the highest yield so far reported. Expression of a plasmid-encoded fusion protein and segregation of the plasmid during bacterial growth here both stable during, fed-batch culture. More than 75 % of the cells retained the plasmid for 130 h under antibiotic-free conditions. In addition, the fusion protein permitting the display of a specific protein on the BMP surface was also stably expressed." @default.
- W4243363525 created "2022-05-12" @default.
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- W4243363525 date "2001-01-01" @default.
- W4243363525 modified "2023-09-30" @default.
- W4243363525 title "Iron Feeding Optimization and Plasmid Stability in Production of Recombinant Bacterial Magnetic Particles by Magnetospirillum magneticum AMB-1 in Fed-Batch Culture." @default.
- W4243363525 doi "https://doi.org/10.1263/jbb.91.213" @default.
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