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• W4243485374 abstract "A microtitre plate nucleic acid probe hybridization system was developed for the detection of ribosomal RNA from thermophilic Campylobacter (Camp. jejuni, Camp. coli, Camp. lari and Camp. upsaliensis). A specific DNA probe obtained by amplification of 23S rRNA sequences using the polymerase chain reaction technique was immobilized on a microtitre plate, and used for hybridization with target 23S rRNA from cell lysates. The RNA‐DNA hybrids thus formed in the wells were detected by an immunoenzymatic assay using a monoclonal antiRNA‐DNA hybrid antibody. The sensitivity of this system was 2.7 x 104 cells ml‐1. This simple, sensitive and inexpensive hybridization and immunoenzymatic assay system should facilitate the detection of Campylobacter in food and clinical samples." @default.
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• W4243485374 date "1997-02-01" @default.
• W4243485374 modified "2023-09-30" @default.
• W4243485374 title "Microtitre plate hybridization system for detection of thermophilic Campylobacter rRNA" @default.
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• W4243485374 doi "https://doi.org/10.1111/j.1365-2672.1997.tb02860.x" @default.
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