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- W4243591630 abstract "The uptake of leucine by S14, an unidentified marine Gram-negative bacterium, was studied during a starvation period of 96 h. The S14 cells displayed two separate uptake systems with different affinities for leucine. The Km values of these systems were 0.76 μM and 20 μM, respectively. The time of exposure to starvation had a marked effect on both uptake systems, not by changing the affinity for leucine, but rather by altering the velocity of uptake (Vmax). A marked increase in the uptake capacity was noted for the high-affinity system, whereas the uptake velocity decreased for the low-affinity system. An osmotic shock treatment resulted in an almost complete loss of substrate binding activity. A gradual recovery of the leucine uptake subsequent to the osmotic shock was observed during a 72-h period of starvation. Separation of the osmotic shock supernatant by gel filtration revealed two proteins, 37 and 44 kDa in size, with leucine binding activity." @default.
- W4243591630 created "2022-05-12" @default.
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- W4243591630 date "1987-08-01" @default.
- W4243591630 modified "2023-09-27" @default.
- W4243591630 title "Uptake of leucine by a marine Gram-negative heterotrophic bacterium during exposure to starvation conditions" @default.
- W4243591630 doi "https://doi.org/10.1016/0378-1097(87)90057-7" @default.
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