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- W4243704570 abstract "Abstract Background: Cryopreservation is the best way for long-term in vitro preservation of plant germplasm resources. The preliminary studies found that reactive oxygen species (ROS) induced oxidative stress and ice-induced membrane damage are the fundamental causes of cell death in cryopreserved samples. How to improve plant cryopreservation survival rate is an important scientific issue in the cryobiology field. Results: This study found that the survival rate was significantly improved by adding single-wall carbon nanotubes (SWCNTs) to plant vitrification solution (PVS) in cryopreservation of Agapanthus praecox embryogenic callus (EC), and analyzed the oxidative response of cells during the control and SWCNTs-added cryopreservation protocol. The SWCNTs entered EC at the step of dehydration, and mainly located around the cell wall and in the vesicles, and most of SWCNTs moved out of EC during dilution step. Combination with physiological index and gene quantitative expression results, SWCNTs affect ROS signal transduction and antioxidant system response during plant cryopreservation. The EC treated by SWCNTs had higher antioxidant levels, like POD, CAT and GSH than the control group EC. EC mainly depended on AsA-GSH and GPX cycle to scavenge H 2 O 2 in the control cryopreservation, but depended on CAT in the SWCNTs-added cryopreservation which lead to low levels of H 2 O 2 and MDA. Elevated antioxidant level in dehydration by adding SWCNTs enhanced cells resistance to injury during cryopreservation. The ROS signals of EC were balanced and stable in the SWCNTs-added cryopreservation. Conclusions: SWCNTs regulated oxidative stress responses of EC during the process, and controlled oxidative damages by the maintenance of ROS homeostasis to achieve high survival rate after cryopreservation. This study is the first to systematically describe the role of carbon nanomaterial in the regulation of plant oxidative stress response, and provided a novel insight into the application of nanomaterials in the field of cryobiology." @default.
- W4243704570 created "2022-05-12" @default.
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- W4243704570 date "2020-06-30" @default.
- W4243704570 modified "2023-09-23" @default.
- W4243704570 title "Single-wall Carbon Nanotubes Improve Cell Survival Rate and Reduce Oxidative Injury in Cryopreservation of Agapanthus praecox Embryogenic Callus" @default.
- W4243704570 doi "https://doi.org/10.21203/rs.3.rs-28448/v2" @default.
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