Matches in SemOpenAlex for { <https://semopenalex.org/work/W4243705919> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W4243705919 endingPage "19" @default.
- W4243705919 startingPage "9" @default.
- W4243705919 abstract "Short runs of mononucleotide repeats are present in chloroplast genomes of higher plants. In soybean, rice, and pine, PCR (polymerase chain reaction) with flanking primers has shown that the numbers of A or T residues in such repeats are variable among closely related taxa. Here we describe a set of primers for studying mononucleotide repeat variation in chloroplast DNA of angiosperms where database information is limited. A total of 39 (A)n and (T)n repeats (n 10) were identified in the tobacco chloroplast genome, and DNA sequences encompassing these 39 regions were aligned with orthologous DNA sequences in the databases. Consensus primer pairs were constructed and used to amplify total genomic DNA from a hierarchical set of angiosperms. All 10 primer pairs generated PCR products from members of the Solanaceae, and 8 of the 10 were also functional in most other angiosperm species. Levels of interspecific polymorphism within the genera Nicotiana, Lycopersicon (both Solanaceae), and Actinidia (Actinidiaceae) proved to be high, while intraspecific variation in Nicotiana tabacum, Lycopersicon esculentum, and Actinidia chinensis was limited. Sequence analysis of PCR products from three primer pairs revealed variable numbers of A, G, and T residues in mononucleotide arrays as the major cause of polymorphism in Actinidia. Our results suggest that universal primers targeted to mononucleotide repeats may serve as general tools to study chloroplast variation in angiosperms.Key words: genetic markers, chloroplast genome, microsatellites, consensus primers, angiosperms." @default.
- W4243705919 created "2022-05-12" @default.
- W4243705919 creator A5028442094 @default.
- W4243705919 creator A5041600050 @default.
- W4243705919 date "1999-01-01" @default.
- W4243705919 modified "2023-10-17" @default.
- W4243705919 title "A set of conserved PCR primers for the analysis of simple sequence repeat polymorphisms in chloroplast genomes of dicotyledonous angiosperms" @default.
- W4243705919 doi "https://doi.org/10.1139/gen-42-1-9" @default.
- W4243705919 hasPublicationYear "1999" @default.
- W4243705919 type Work @default.
- W4243705919 citedByCount "443" @default.
- W4243705919 countsByYear W42437059192012 @default.
- W4243705919 countsByYear W42437059192013 @default.
- W4243705919 countsByYear W42437059192014 @default.
- W4243705919 countsByYear W42437059192015 @default.
- W4243705919 countsByYear W42437059192016 @default.
- W4243705919 countsByYear W42437059192017 @default.
- W4243705919 countsByYear W42437059192018 @default.
- W4243705919 countsByYear W42437059192019 @default.
- W4243705919 countsByYear W42437059192020 @default.
- W4243705919 countsByYear W42437059192021 @default.
- W4243705919 countsByYear W42437059192022 @default.
- W4243705919 countsByYear W42437059192023 @default.
- W4243705919 crossrefType "journal-article" @default.
- W4243705919 hasAuthorship W4243705919A5028442094 @default.
- W4243705919 hasAuthorship W4243705919A5041600050 @default.
- W4243705919 hasConcept C104317684 @default.
- W4243705919 hasConcept C141231307 @default.
- W4243705919 hasConcept C178790620 @default.
- W4243705919 hasConcept C180754005 @default.
- W4243705919 hasConcept C185592680 @default.
- W4243705919 hasConcept C2777362759 @default.
- W4243705919 hasConcept C2777563447 @default.
- W4243705919 hasConcept C2781205006 @default.
- W4243705919 hasConcept C48050816 @default.
- W4243705919 hasConcept C51679486 @default.
- W4243705919 hasConcept C54355233 @default.
- W4243705919 hasConcept C552990157 @default.
- W4243705919 hasConcept C59822182 @default.
- W4243705919 hasConcept C61320498 @default.
- W4243705919 hasConcept C86803240 @default.
- W4243705919 hasConceptScore W4243705919C104317684 @default.
- W4243705919 hasConceptScore W4243705919C141231307 @default.
- W4243705919 hasConceptScore W4243705919C178790620 @default.
- W4243705919 hasConceptScore W4243705919C180754005 @default.
- W4243705919 hasConceptScore W4243705919C185592680 @default.
- W4243705919 hasConceptScore W4243705919C2777362759 @default.
- W4243705919 hasConceptScore W4243705919C2777563447 @default.
- W4243705919 hasConceptScore W4243705919C2781205006 @default.
- W4243705919 hasConceptScore W4243705919C48050816 @default.
- W4243705919 hasConceptScore W4243705919C51679486 @default.
- W4243705919 hasConceptScore W4243705919C54355233 @default.
- W4243705919 hasConceptScore W4243705919C552990157 @default.
- W4243705919 hasConceptScore W4243705919C59822182 @default.
- W4243705919 hasConceptScore W4243705919C61320498 @default.
- W4243705919 hasConceptScore W4243705919C86803240 @default.
- W4243705919 hasIssue "1" @default.
- W4243705919 hasLocation W42437059191 @default.
- W4243705919 hasOpenAccess W4243705919 @default.
- W4243705919 hasPrimaryLocation W42437059191 @default.
- W4243705919 hasRelatedWork W1531257505 @default.
- W4243705919 hasRelatedWork W1548811127 @default.
- W4243705919 hasRelatedWork W1594933590 @default.
- W4243705919 hasRelatedWork W2044438529 @default.
- W4243705919 hasRelatedWork W2064291183 @default.
- W4243705919 hasRelatedWork W2090139400 @default.
- W4243705919 hasRelatedWork W2189381471 @default.
- W4243705919 hasRelatedWork W2295707821 @default.
- W4243705919 hasRelatedWork W2301943644 @default.
- W4243705919 hasRelatedWork W2373996679 @default.
- W4243705919 hasVolume "42" @default.
- W4243705919 isParatext "false" @default.
- W4243705919 isRetracted "false" @default.
- W4243705919 workType "article" @default.