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• W4243946781 abstract "Since 2012, when clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) systems were first adapted to use as programmable genome-editing tools, editing technology has undergone an extraordinary degree of evolution, going far beyond the paradigm of inducing double-strand breaks and cellular repair of the breaks, originally enabled by zinc-finger nucleases and then continued with transcription activator-like effector nucleases and CRISPR. This chapter and the next chapter discuss the variety of new approaches now available to make precise changes to genes or perturbations of gene expression. The focus of this chapter is base editing, which has the ability to make specific base pair changes at specific sites in the genome in a manner that avoids the need for double-strand breaks altogether and instead relies on direct chemical modification of DNA bases." @default.
• W4243946781 created "2022-05-12" @default.
• W4243946781 creator A5040568538 @default.
• W4243946781 date "2021-01-01" @default.
• W4243946781 modified "2023-09-30" @default.
• W4243946781 title "Base editing" @default.
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• W4243946781 doi "https://doi.org/10.1016/b978-0-12-823484-6.00009-8" @default.
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