Matches in SemOpenAlex for { <https://semopenalex.org/work/W4244173181> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W4244173181 endingPage "338" @default.
- W4244173181 startingPage "332" @default.
- W4244173181 abstract "In the myelodysplastic syndrome (MDS), cytogenetic abnormalities are often present and can be used as markers in studies for cell lineage involvement. Little is known of the involvement of the megakaryocytic lineage due to the variable ploidy of these cells. We applied dual-color fluorescence in situ hybridization (FISH) to routinely prepared bone marrow (BM) smears of cytogenetically normal patients and seven patients with MDS and monosomy 7 or trisomy 8. Probes specific for the centromeric regions of chromosomes 7 and 8 were detected with fluorescein isothiocyanate (FITC) and Texas Red, respectively. This enabled us to assess the ratio between the numbers of chromosomes 7 and 8 in the polyploid cells. We utilized confocal laser scanning microscopy to count the FITC and Texas Red FISH signals in the different focal layers of the megakaryocytes. Fifty-six megakaryocytes in six normal BM smears were analyzed, giving a mean ratio of 1.0, a standard deviation (SD) of 0.12, and a range of 0.8–1.33. This ratio was applied to evaluation of clonal involvement of individual megakaryocytes in the patients with MDS. In two patients with monosomy 7, the majority of the megakaryocytes were monosomic. In the five patients with trisomy 8, all or a majority of the analyzed megakaryocytes were trisomic. These results add direct evidence that in MDS megakaryocytes are involved in the malignant clone. Genes Chromosomes Cancer 25:332–338, 1999. © 1999 Wiley-Liss, Inc." @default.
- W4244173181 created "2022-05-12" @default.
- W4244173181 creator A5028119059 @default.
- W4244173181 creator A5046775582 @default.
- W4244173181 creator A5047299605 @default.
- W4244173181 creator A5069691714 @default.
- W4244173181 creator A5089249551 @default.
- W4244173181 date "1999-08-01" @default.
- W4244173181 modified "2023-10-03" @default.
- W4244173181 title "Cytogenetic clonality analysis of megakaryocytes in myelodysplastic syndrome by dual‐color fluorescence in situ hybridization and confocal laser scanning microscopy" @default.
- W4244173181 doi "https://doi.org/10.1002/(sici)1098-2264(199908)25:4<332::aid-gcc4>3.3.co;2-w" @default.
- W4244173181 hasPublicationYear "1999" @default.
- W4244173181 type Work @default.
- W4244173181 citedByCount "1" @default.
- W4244173181 countsByYear W42441731812021 @default.
- W4244173181 crossrefType "journal-article" @default.
- W4244173181 hasAuthorship W4244173181A5028119059 @default.
- W4244173181 hasAuthorship W4244173181A5046775582 @default.
- W4244173181 hasAuthorship W4244173181A5047299605 @default.
- W4244173181 hasAuthorship W4244173181A5069691714 @default.
- W4244173181 hasAuthorship W4244173181A5089249551 @default.
- W4244173181 hasConcept C104317684 @default.
- W4244173181 hasConcept C142724271 @default.
- W4244173181 hasConcept C153911025 @default.
- W4244173181 hasConcept C174475383 @default.
- W4244173181 hasConcept C201750760 @default.
- W4244173181 hasConcept C203014093 @default.
- W4244173181 hasConcept C2776229224 @default.
- W4244173181 hasConcept C2777542201 @default.
- W4244173181 hasConcept C2779142324 @default.
- W4244173181 hasConcept C2779672484 @default.
- W4244173181 hasConcept C2779705218 @default.
- W4244173181 hasConcept C2780007613 @default.
- W4244173181 hasConcept C2780365373 @default.
- W4244173181 hasConcept C2780817109 @default.
- W4244173181 hasConcept C28328180 @default.
- W4244173181 hasConcept C30481170 @default.
- W4244173181 hasConcept C53226629 @default.
- W4244173181 hasConcept C54355233 @default.
- W4244173181 hasConcept C71924100 @default.
- W4244173181 hasConcept C81089528 @default.
- W4244173181 hasConcept C86803240 @default.
- W4244173181 hasConceptScore W4244173181C104317684 @default.
- W4244173181 hasConceptScore W4244173181C142724271 @default.
- W4244173181 hasConceptScore W4244173181C153911025 @default.
- W4244173181 hasConceptScore W4244173181C174475383 @default.
- W4244173181 hasConceptScore W4244173181C201750760 @default.
- W4244173181 hasConceptScore W4244173181C203014093 @default.
- W4244173181 hasConceptScore W4244173181C2776229224 @default.
- W4244173181 hasConceptScore W4244173181C2777542201 @default.
- W4244173181 hasConceptScore W4244173181C2779142324 @default.
- W4244173181 hasConceptScore W4244173181C2779672484 @default.
- W4244173181 hasConceptScore W4244173181C2779705218 @default.
- W4244173181 hasConceptScore W4244173181C2780007613 @default.
- W4244173181 hasConceptScore W4244173181C2780365373 @default.
- W4244173181 hasConceptScore W4244173181C2780817109 @default.
- W4244173181 hasConceptScore W4244173181C28328180 @default.
- W4244173181 hasConceptScore W4244173181C30481170 @default.
- W4244173181 hasConceptScore W4244173181C53226629 @default.
- W4244173181 hasConceptScore W4244173181C54355233 @default.
- W4244173181 hasConceptScore W4244173181C71924100 @default.
- W4244173181 hasConceptScore W4244173181C81089528 @default.
- W4244173181 hasConceptScore W4244173181C86803240 @default.
- W4244173181 hasIssue "4" @default.
- W4244173181 hasLocation W42441731811 @default.
- W4244173181 hasOpenAccess W4244173181 @default.
- W4244173181 hasPrimaryLocation W42441731811 @default.
- W4244173181 hasRelatedWork W1974754966 @default.
- W4244173181 hasRelatedWork W1979015094 @default.
- W4244173181 hasRelatedWork W2150137793 @default.
- W4244173181 hasRelatedWork W215965135 @default.
- W4244173181 hasRelatedWork W2406168547 @default.
- W4244173181 hasRelatedWork W2409379738 @default.
- W4244173181 hasRelatedWork W2414698859 @default.
- W4244173181 hasRelatedWork W2417351798 @default.
- W4244173181 hasRelatedWork W35686571 @default.
- W4244173181 hasRelatedWork W4244173181 @default.
- W4244173181 hasVolume "25" @default.
- W4244173181 isParatext "false" @default.
- W4244173181 isRetracted "false" @default.
- W4244173181 workType "article" @default.