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- W4244301067 abstract "Fumarase catalysed hydration of fumarate was investigated in water/organic solvent one-phase systems. The organic solvents used were ethylene glycol, glycerol and dimethylformamide. The effects of the amount of organic solvent on the maximum velocity (Vmax), the Michaelis-Menten constant (KM) and the equilibrium constant (Keq) were studied in all the reaction media. Together with a denaturing power of the solvent evidenced by a systematic decrease of Vmax also a surprising decrease of the KM was registered as the percentage of organic solvent in the reaction media was increased. While the equilibrium constant of the reaction (Keq = [l-malate]/[fumarate]) decreased when the percentage of organic solvent was raised. An interpretation of these facts was given. Time-dependent denaturation was also investigated and glycerol resulted the less denaturing of the solvents used, while the aprotic DMF exhibited the highest deactivation." @default.
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- W4244301067 date "1996-06-01" @default.
- W4244301067 modified "2023-09-30" @default.
- W4244301067 title "Study of fumarase activity in non-conventional media. Part I" @default.
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- W4244301067 doi "https://doi.org/10.1016/1381-1177(95)00276-6" @default.
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