Matches in SemOpenAlex for { <https://semopenalex.org/work/W4244626107> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W4244626107 endingPage "58" @default.
- W4244626107 startingPage "58" @default.
- W4244626107 abstract "Inositol 1,4,5-trisphosphate receptor (IP3R) plays a pivotal role in the Ca2+ release process in a variety of cell types. Additionally, IP3R is distributed in ventricular intercalated discs, but its function(s) in this particular site remains unknown. Connexin (Cx43), the predominant gap junction (GJ) protein in ventricular myocardium, is linked to several signaling pathways that regulate Cx43 properties by (de)phosphorylation on multiple residues. Here, we investigated the regulatory role of IP3R in cell-cell communication and the mechanism(s) underlying this effect. In neonatal rat and adult mouse ventricular myocytes IP3R co-localized and co-immunoprecipitated with Cx43 in GJ plaques detected by immunostaining and western blot assays. Blocking IP3R with antagonists or silencing pan-IP3R expression with shRNA hindered the 6-carboxyfluorescein (6-CFDA) diffusion through GJs and desynchronized Ca2+ transients among confluent neonatal myocytes in culture, whereas stimulation of IP3R with IP3 ester or ATP exerted the opposite effect. Likewise, 6-CFDA propagation through GJs was modulated by IP3R activation or inhibition in cell pairs of isolated adult cardiomyocytes. Furthermore, IP3R activation or IP3R suppression promoted or suppressed, respectively, Cx43 phosphorylation on S279/282. Site-directed mutagenesis indicated that expression of a mutant Cx43-S282A (alanine) inhibited S279/282 phosphorylation and GJ permeability, while the S279A mutant showed the opposite effect in ventricular myocytes. Expression of these mutants in HEK293 cells revealed that cells with a dual S279/282 mutation failed to express exogenous Cx43, whereas cells with a single S279 or S282 mutation displayed Cx43 overexpression with increased phosphorylation of S279/282 and promotion of intercellular communication. These results demonstrated, for the first time, that IP3R physically interacts with Cx43 and participates in the regulation of Cx43 phosphorylation on S279/282, thereby affecting GJ intercellular communication in ventricular myocytes." @default.
- W4244626107 created "2022-05-12" @default.
- W4244626107 creator A5001001450 @default.
- W4244626107 creator A5026098740 @default.
- W4244626107 creator A5032447166 @default.
- W4244626107 creator A5046234506 @default.
- W4244626107 creator A5048903877 @default.
- W4244626107 creator A5063424279 @default.
- W4244626107 creator A5066215262 @default.
- W4244626107 creator A5070570599 @default.
- W4244626107 creator A5079104664 @default.
- W4244626107 creator A5085529129 @default.
- W4244626107 creator A5089966579 @default.
- W4244626107 date "2014-01-01" @default.
- W4244626107 modified "2023-09-27" @default.
- W4244626107 title "Cx43 phosphorylation on S279/282 and intercellular communication are regulated by IP 3 /IP 3 receptor signaling" @default.
- W4244626107 doi "https://doi.org/10.1186/preaccept-9317128261148505" @default.
- W4244626107 hasPublicationYear "2014" @default.
- W4244626107 type Work @default.
- W4244626107 citedByCount "0" @default.
- W4244626107 crossrefType "journal-article" @default.
- W4244626107 hasAuthorship W4244626107A5001001450 @default.
- W4244626107 hasAuthorship W4244626107A5026098740 @default.
- W4244626107 hasAuthorship W4244626107A5032447166 @default.
- W4244626107 hasAuthorship W4244626107A5046234506 @default.
- W4244626107 hasAuthorship W4244626107A5048903877 @default.
- W4244626107 hasAuthorship W4244626107A5063424279 @default.
- W4244626107 hasAuthorship W4244626107A5066215262 @default.
- W4244626107 hasAuthorship W4244626107A5070570599 @default.
- W4244626107 hasAuthorship W4244626107A5079104664 @default.
- W4244626107 hasAuthorship W4244626107A5085529129 @default.
- W4244626107 hasAuthorship W4244626107A5089966579 @default.
- W4244626107 hasBestOaLocation W42446261071 @default.
- W4244626107 hasConcept C104317684 @default.
- W4244626107 hasConcept C11960822 @default.
- W4244626107 hasConcept C143065580 @default.
- W4244626107 hasConcept C152624196 @default.
- W4244626107 hasConcept C153911025 @default.
- W4244626107 hasConcept C158157758 @default.
- W4244626107 hasConcept C170493617 @default.
- W4244626107 hasConcept C174510640 @default.
- W4244626107 hasConcept C185592680 @default.
- W4244626107 hasConcept C207200792 @default.
- W4244626107 hasConcept C2777427919 @default.
- W4244626107 hasConcept C2780228694 @default.
- W4244626107 hasConcept C55493867 @default.
- W4244626107 hasConcept C62478195 @default.
- W4244626107 hasConcept C79879829 @default.
- W4244626107 hasConcept C86803240 @default.
- W4244626107 hasConcept C95444343 @default.
- W4244626107 hasConceptScore W4244626107C104317684 @default.
- W4244626107 hasConceptScore W4244626107C11960822 @default.
- W4244626107 hasConceptScore W4244626107C143065580 @default.
- W4244626107 hasConceptScore W4244626107C152624196 @default.
- W4244626107 hasConceptScore W4244626107C153911025 @default.
- W4244626107 hasConceptScore W4244626107C158157758 @default.
- W4244626107 hasConceptScore W4244626107C170493617 @default.
- W4244626107 hasConceptScore W4244626107C174510640 @default.
- W4244626107 hasConceptScore W4244626107C185592680 @default.
- W4244626107 hasConceptScore W4244626107C207200792 @default.
- W4244626107 hasConceptScore W4244626107C2777427919 @default.
- W4244626107 hasConceptScore W4244626107C2780228694 @default.
- W4244626107 hasConceptScore W4244626107C55493867 @default.
- W4244626107 hasConceptScore W4244626107C62478195 @default.
- W4244626107 hasConceptScore W4244626107C79879829 @default.
- W4244626107 hasConceptScore W4244626107C86803240 @default.
- W4244626107 hasConceptScore W4244626107C95444343 @default.
- W4244626107 hasIssue "1" @default.
- W4244626107 hasLocation W42446261071 @default.
- W4244626107 hasLocation W42446261072 @default.
- W4244626107 hasOpenAccess W4244626107 @default.
- W4244626107 hasPrimaryLocation W42446261071 @default.
- W4244626107 hasRelatedWork W2003142024 @default.
- W4244626107 hasRelatedWork W2036548518 @default.
- W4244626107 hasRelatedWork W2039235242 @default.
- W4244626107 hasRelatedWork W2086770062 @default.
- W4244626107 hasRelatedWork W2120843887 @default.
- W4244626107 hasRelatedWork W2121730220 @default.
- W4244626107 hasRelatedWork W2292296809 @default.
- W4244626107 hasRelatedWork W2515245704 @default.
- W4244626107 hasRelatedWork W4244626107 @default.
- W4244626107 hasRelatedWork W2049490721 @default.
- W4244626107 hasVolume "12" @default.
- W4244626107 isParatext "false" @default.
- W4244626107 isRetracted "false" @default.
- W4244626107 workType "article" @default.