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- W4244666988 abstract "Redox modulation participates in the regulation of intracellular free calcium concentration ([Ca2+]i) in several cell types. In thyroid cells, including FRTL-5 cells, changes in [Ca2+]i regulate several important functions, including the production of H2O2 (hydrogen peroxide). As H2O2 is of crucial importance for the production of thyroid hormones, we investigated the effects of H2O2 on [Ca2+]i in thyroid FRTL-5 cells. H2O2 itself did not modulate basal [Ca2+]i. However, H2O2 attenuated store-operated calcium entry evoked by thapsigargin, both in a sodium-containing buffer and in a sodium-free buffer. The effect of H2O2 was abrogated by the reducing agent β-mercaptoethanol. H2O2 also attenuated the thapsigargin-evoked entry of barium and manganese. The effect of H2O2 was, at least in part, mediated by activation of protein kinase C (PKC), as H2O2 enhanced the binding of [3H]phorbol 12,13-dibutyrate. H2O2 also stimulated the translocation of the isoenzyme PKCε from the cytosolic fraction to the particulate fraction. Furthermore, H2O2 did not attenuate store-operated calcium entry in cells treated with staurosporine or calphostin C, or in cells with down-regulated PKC. H2O2 depolarized the membrane potential in bisoxonol-loaded cells and when patch-clamp in the whole-cell mode was used. The depolarization was attenuated in cells with down-regulated PKC. This depolarization, at least in part, explained the H2O2-evoked inhibition of calcium entry. In addition, H2O2 enhanced the extrusion of calcium from cells stimulated with thapsigargin and this effect was abolished in cells with down-regulated PKC and after treatment of the cells with the reducing agent β-mercaptoethanol. In conclusion H2O2 attenuates an increase in [Ca2+]i. As H2O2 is produced in thyroid cells in a calcium-dependent manner, our results suggest that H2O2 may participate in the regulation of [Ca2+]i in these cells via a negative-feedback mechanism involving activation of PKC." @default.
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- W4244666988 date "2000-09-26" @default.
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- W4244666988 title "Hydrogen peroxide attenuates store-operated calcium entry and enhances calcium extrusion in thyroid FRTL-5 cells" @default.
- W4244666988 doi "https://doi.org/10.1042/bj3510047" @default.
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