FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4244873904> ?p ?o ?g. }

• W4244873904 endingPage "S114" @default.
• W4244873904 startingPage "S114" @default.
• W4244873904 abstract "RATIONALE: An inactivated influenza vaccine (Optaflu® is manufactured by Novartis, registered in the EU) produced in canine kidney cells (MDCK 33016-PF) contains no egg proteins and may be used for immunizing egg-allergic patients. Although no major dog allergens were identified in MDCK 33016-PF cells, minor dog allergens might be present and cause reactions in dog-allergic individuals. We sought to evaluate allergenicity of Optaflu in a passive sensitization assay.METHODS: RBL cells transfected with human IgE receptor-1 were sensitized with sera from adult dog-allergic subjects with positive skin prick tests to dog extract and detectable dog dander -IgE, and stimulated with serial dilutions of Optaflu and dog dander extract. N-hexosaminidase release% (NHR) was used as a marker of RBL degranulation. Western blots were performed and UniCAP was used to measure dog-specific IgE antibody levels.RESULTS: Median dog dander-IgE was 8.31 kUA/L (0.62; 67.9); median dog epithelium-IgE was 3.2 kUA/L, (0.4; 38.2). Median (range) maximum NHR (at 1st 10-fold dilution) was: 1). Optaflu- 0% (0; 1.4); 2). Dog dander-10.2% (0; 35.9), P < 0.001; and 3). Anti-IgE positive control-17.7% (1.2; 45.4). In a control egg-allergic subject, maximum NHR to a vaccine cultured in chick embryo and containing egg protein was 10.2%. IgE antibodies in pooled sera did not bind to Optaflu on immunoblots but produced strong binding to dog dander and epithelium extracts. Serum from a control egg-allergic subject strongly bound embryonated egg-derived vaccine.CONCLUSIONS: Optaflu, an influenza vaccine produced in continuous canine kidney cell line did not trigger degranulation in RBL cells passively sensitized with human anti-dog IgE. RATIONALE: An inactivated influenza vaccine (Optaflu® is manufactured by Novartis, registered in the EU) produced in canine kidney cells (MDCK 33016-PF) contains no egg proteins and may be used for immunizing egg-allergic patients. Although no major dog allergens were identified in MDCK 33016-PF cells, minor dog allergens might be present and cause reactions in dog-allergic individuals. We sought to evaluate allergenicity of Optaflu in a passive sensitization assay. METHODS: RBL cells transfected with human IgE receptor-1 were sensitized with sera from adult dog-allergic subjects with positive skin prick tests to dog extract and detectable dog dander -IgE, and stimulated with serial dilutions of Optaflu and dog dander extract. N-hexosaminidase release% (NHR) was used as a marker of RBL degranulation. Western blots were performed and UniCAP was used to measure dog-specific IgE antibody levels. RESULTS: Median dog dander-IgE was 8.31 kUA/L (0.62; 67.9); median dog epithelium-IgE was 3.2 kUA/L, (0.4; 38.2). Median (range) maximum NHR (at 1st 10-fold dilution) was: 1). Optaflu- 0% (0; 1.4); 2). Dog dander-10.2% (0; 35.9), P < 0.001; and 3). Anti-IgE positive control-17.7% (1.2; 45.4). In a control egg-allergic subject, maximum NHR to a vaccine cultured in chick embryo and containing egg protein was 10.2%. IgE antibodies in pooled sera did not bind to Optaflu on immunoblots but produced strong binding to dog dander and epithelium extracts. Serum from a control egg-allergic subject strongly bound embryonated egg-derived vaccine. CONCLUSIONS: Optaflu, an influenza vaccine produced in continuous canine kidney cell line did not trigger degranulation in RBL cells passively sensitized with human anti-dog IgE." @default.
• W4244873904 created "2022-05-12" @default.
• W4244873904 creator A5005335627 @default.
• W4244873904 creator A5052548559 @default.
• W4244873904 creator A5066056325 @default.
• W4244873904 creator A5084256551 @default.
• W4244873904 date "2009-02-01" @default.
• W4244873904 modified "2023-09-25" @default.
• W4244873904 title "In vitro Assessment of the Allergenicity of Novel Influenza Vaccine Produced in Dog Kidney Cells in Subjects with Dog Allergy" @default.
• W4244873904 doi "https://doi.org/10.1016/j.jaci.2008.12.417" @default.
• W4244873904 hasPublicationYear "2009" @default.
• W4244873904 type Work @default.
• W4244873904 citedByCount "1" @default.
• W4244873904 crossrefType "journal-article" @default.
• W4244873904 hasAuthorship W4244873904A5005335627 @default.
• W4244873904 hasAuthorship W4244873904A5052548559 @default.
• W4244873904 hasAuthorship W4244873904A5066056325 @default.
• W4244873904 hasAuthorship W4244873904A5084256551 @default.
• W4244873904 hasBestOaLocation W42448739041 @default.
• W4244873904 hasConcept C126322002 @default.
• W4244873904 hasConcept C141105273 @default.
• W4244873904 hasConcept C159654299 @default.
• W4244873904 hasConcept C170493617 @default.
• W4244873904 hasConcept C203014093 @default.
• W4244873904 hasConcept C207480886 @default.
• W4244873904 hasConcept C49802076 @default.
• W4244873904 hasConcept C71924100 @default.
• W4244873904 hasConcept C74962801 @default.
• W4244873904 hasConcept C86803240 @default.
• W4244873904 hasConceptScore W4244873904C126322002 @default.
• W4244873904 hasConceptScore W4244873904C141105273 @default.
• W4244873904 hasConceptScore W4244873904C159654299 @default.
• W4244873904 hasConceptScore W4244873904C170493617 @default.
• W4244873904 hasConceptScore W4244873904C203014093 @default.
• W4244873904 hasConceptScore W4244873904C207480886 @default.
• W4244873904 hasConceptScore W4244873904C49802076 @default.
• W4244873904 hasConceptScore W4244873904C71924100 @default.
• W4244873904 hasConceptScore W4244873904C74962801 @default.
• W4244873904 hasConceptScore W4244873904C86803240 @default.
• W4244873904 hasIssue "2" @default.
• W4244873904 hasLocation W42448739041 @default.
• W4244873904 hasOpenAccess W4244873904 @default.
• W4244873904 hasPrimaryLocation W42448739041 @default.
• W4244873904 hasRelatedWork W1661858862 @default.
• W4244873904 hasRelatedWork W1980244606 @default.
• W4244873904 hasRelatedWork W2005452495 @default.
• W4244873904 hasRelatedWork W2016392084 @default.
• W4244873904 hasRelatedWork W2027510281 @default.
• W4244873904 hasRelatedWork W2067605016 @default.
• W4244873904 hasRelatedWork W2080227842 @default.
• W4244873904 hasRelatedWork W2561511743 @default.
• W4244873904 hasRelatedWork W2948080223 @default.
• W4244873904 hasRelatedWork W3121682850 @default.
• W4244873904 hasVolume "123" @default.
• W4244873904 isParatext "false" @default.
• W4244873904 isRetracted "false" @default.
• W4244873904 workType "article" @default.