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- W42449013 abstract "A kinetic study has been made of the effects of 5-bromouracil deoxyriboside (BrUDR) on T2 development in a sulfanilamide-containing medium. As tested by the u.v. light sensitivity of the intracellularly developing phage and by the time of appearance of the first intracellular mature phages, it was found that sulfanilamide and BrUDR did not change the pattern of phage development, but slowed it down slightly. Sulfanilamide caused lysis inhibition of the r2 pahges in this medium. BrUDR did not begin to be effective as a mutagen until the time expected for commencement of phage DNA synthesis, but the mutagenic action of BrUDR could be exerted at any time thereafter as long as phage DNA continued to be synthesized. Counteraction of free BrUDR by thymidine at 15 min after infection did not halt the continued production of mutants. It is proposed that mutations are induced both in the process of the introduction of BrU into phage DNA and during the replication of DNA containing BrU. Two mechanisms by which BrU might induce the appearance of altered base sequences in phage DNA are outlined." @default.
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- W42449013 date "1960-01-01" @default.
- W42449013 modified "2023-09-27" @default.
- W42449013 title "The induction of mutants of bacteriophage T2 by 5-bromouracil" @default.
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- W42449013 doi "https://doi.org/10.1016/0006-3002(60)90759-9" @default.
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