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- W4244996919 abstract "A laboratory study was carried out to evaluate the impact of the introduction of genetically modified microorganisms into soil, in terms of effect on the diversity of the indigenous microflora, and at the process level. The impact on microbial phenotypic diversity, and on soil denitrification of an inoculum of a lux-modified denitrifier, Pseudomonas fluorescens, was examined using two different soil types in re-packed soil microcosms. The effect on diversity was found to depend on the soil pore size class into which the modified inoculum was introduced. The introduction of lux-modified cells into the 15–30 μm pore neck size class caused a short-term reduction in the overall microbial diversity. There was no significant change in the diversity of the indigenous microbial community, however, when cells were introduced into the 40–60 μm pore class. Partial chloroform fumigation proved useful in differentiating cell populations with respect to pore location. No change in diversity was observed when dead cells (either heat killed or glutaraldehyde fixed) were introduced into either pore size class. At the process level, the effect on soil denitrification of introduction of lux-modified P. fluorescens was not significantly different from introduction of the equivalent inoculum of the parental wild-type, although denitrification was found to be dependent upon both soil structure and pore size location of the introduced inoculum." @default.
- W4244996919 created "2022-05-12" @default.
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- W4244996919 date "1994-06-01" @default.
- W4244996919 modified "2023-09-26" @default.
- W4244996919 title "Effect of an introduced inoculum on soil microbial diversity" @default.
- W4244996919 doi "https://doi.org/10.1016/0168-6496(94)90094-9" @default.
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