Matches in SemOpenAlex for { <https://semopenalex.org/work/W4245035269> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W4245035269 abstract "Abstract Background: Alpha actinins (ACTNs) are major cytoskeletal proteins and exhibit many non-muscle functions. Emerging evidence have uncovered the regulatory role of ACTNs in tumorigenesis, however, the expression pattern, biological functions, and underlying mechanism of ACTN1 in hepatocellular carcinoma (HCC) remain largely unexplored. Methods: Immunohistochemical analysis of a HCC tissue microarray (n = 157) was performed to determine the expression pattern and prognostic value of ACTN1 in HCC. In vitro loss-of-function study in HCC cells were carried out to investigate ACTN1 knockdown on cell proliferation. In vivo subcutaneous xenograft model and intrahepatic transplantation model were generated to decipher the contribution of ACTN1 in the tumor growth of HCC. Gene set enrichment analysis, quantitative real-time PCR, Co-immunoprecipitation, immunofluorescence and western blotting were performed to identify the underlying molecular mechanism. Results: It was found that ACTN1 was significantly upregulated in HCC tissues and closely related to llpha-fetoprotein level, tumor thrombus, tumor size, TNM stage and patient prognoses. Knockdown of ACTN1 suppressed in vitro cell proliferation and in vivo tumor growth of HCC cells. Mechanistically, knockdown of ACTN1 increased Hippo signaling pathway activity and decrease Rho GTPases activities. Mechanistically, ACTN1 could competitively interact with MOB1 and decrease the phosphorylation of LATS1 and YAP. The growth-promoting effect induced by ACTN1 was significantly abrogated by pharmacological inhibition of YAP with verteporfin or super-TDU. Conclusions: ACTN1 is highly expressed in HCC tissues and acts as a tumor promoter by suppressing Hippo signaling via physical interaction with MOB1. ACTN1 may serve as a potential prognostic marker and therapeutic target for HCC." @default.
- W4245035269 created "2022-05-12" @default.
- W4245035269 creator A5022744654 @default.
- W4245035269 creator A5036324254 @default.
- W4245035269 creator A5039018137 @default.
- W4245035269 creator A5068290128 @default.
- W4245035269 date "2020-09-10" @default.
- W4245035269 modified "2023-09-30" @default.
- W4245035269 title "ACTN1 Supports Tumor Growth by Inhibiting Hippo Signaling in Hepatocellular Carcinoma" @default.
- W4245035269 doi "https://doi.org/10.21203/rs.3.rs-72190/v1" @default.
- W4245035269 hasPublicationYear "2020" @default.
- W4245035269 type Work @default.
- W4245035269 citedByCount "0" @default.
- W4245035269 crossrefType "posted-content" @default.
- W4245035269 hasAuthorship W4245035269A5022744654 @default.
- W4245035269 hasAuthorship W4245035269A5036324254 @default.
- W4245035269 hasAuthorship W4245035269A5039018137 @default.
- W4245035269 hasAuthorship W4245035269A5068290128 @default.
- W4245035269 hasBestOaLocation W42450352691 @default.
- W4245035269 hasConcept C121608353 @default.
- W4245035269 hasConcept C150903083 @default.
- W4245035269 hasConcept C172512520 @default.
- W4245035269 hasConcept C173396325 @default.
- W4245035269 hasConcept C207001950 @default.
- W4245035269 hasConcept C2778019345 @default.
- W4245035269 hasConcept C502942594 @default.
- W4245035269 hasConcept C54355233 @default.
- W4245035269 hasConcept C55493867 @default.
- W4245035269 hasConcept C555283112 @default.
- W4245035269 hasConcept C62112901 @default.
- W4245035269 hasConcept C62478195 @default.
- W4245035269 hasConcept C81885089 @default.
- W4245035269 hasConcept C86803240 @default.
- W4245035269 hasConcept C95444343 @default.
- W4245035269 hasConceptScore W4245035269C121608353 @default.
- W4245035269 hasConceptScore W4245035269C150903083 @default.
- W4245035269 hasConceptScore W4245035269C172512520 @default.
- W4245035269 hasConceptScore W4245035269C173396325 @default.
- W4245035269 hasConceptScore W4245035269C207001950 @default.
- W4245035269 hasConceptScore W4245035269C2778019345 @default.
- W4245035269 hasConceptScore W4245035269C502942594 @default.
- W4245035269 hasConceptScore W4245035269C54355233 @default.
- W4245035269 hasConceptScore W4245035269C55493867 @default.
- W4245035269 hasConceptScore W4245035269C555283112 @default.
- W4245035269 hasConceptScore W4245035269C62112901 @default.
- W4245035269 hasConceptScore W4245035269C62478195 @default.
- W4245035269 hasConceptScore W4245035269C81885089 @default.
- W4245035269 hasConceptScore W4245035269C86803240 @default.
- W4245035269 hasConceptScore W4245035269C95444343 @default.
- W4245035269 hasLocation W42450352691 @default.
- W4245035269 hasLocation W42450352692 @default.
- W4245035269 hasLocation W42450352693 @default.
- W4245035269 hasOpenAccess W4245035269 @default.
- W4245035269 hasPrimaryLocation W42450352691 @default.
- W4245035269 hasRelatedWork W2150163671 @default.
- W4245035269 hasRelatedWork W2158597407 @default.
- W4245035269 hasRelatedWork W2531142332 @default.
- W4245035269 hasRelatedWork W2600717990 @default.
- W4245035269 hasRelatedWork W2602561919 @default.
- W4245035269 hasRelatedWork W2606878667 @default.
- W4245035269 hasRelatedWork W2903968497 @default.
- W4245035269 hasRelatedWork W2922197439 @default.
- W4245035269 hasRelatedWork W2964133775 @default.
- W4245035269 hasRelatedWork W3087442266 @default.
- W4245035269 isParatext "false" @default.
- W4245035269 isRetracted "false" @default.
- W4245035269 workType "article" @default.