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- W4245126565 abstract "7β-(4-Carboxybutanamido)cephalosporanic acid acylase (penicillin amidohydrolase, EC 3.5.1.11) was crystallized from cell-free extracts of a mutant derived from Pseudomonas SY-77-1. Purification of the enzyme was performed by a procedure involving ammonium sulfate fractionation and column chromatographies on DEAE-Sephadex, TEAE-cellulose and Sephadex G-200. The crystalline enzyme was homogeneous on polyacrylamide gel disc electrophoresis. The molecular weight of the acylase was estimated to be 1.3 × 105 by gel filtration. The enzyme was fully active at pH above 6.5 and was highly stable at a pH range of 6.0 to 8.0 and below 38°C. The Michaelis-Menten constant (Km) and Vmax for 7β-(4-carboxybutanamido)cephalosporanic acid were 0.16mM and 4.91 μmol/min/mg-protein, respectively. It was also indicated that this enzyme-protein occupied 2.3% of the dry-cell weight." @default.
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- W4245126565 date "1981-10-01" @default.
- W4245126565 modified "2023-09-23" @default.
- W4245126565 title "Purification and Properties of 7β-(4-Carboxybutanamido)-cephalosporanic Acid Acylase Produced by Mutants Derived fromPseudomonas" @default.
- W4245126565 doi "https://doi.org/10.1080/00021369.1981.10864878" @default.
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