Matches in SemOpenAlex for { <https://semopenalex.org/work/W4245129849> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W4245129849 abstract "Abstract Background: Increases in seawater temperatures and in the frequency and severity of hypoxic events are expected with climate change, and may become a challenge for cultured Atlantic salmon and negatively affect their growth, immunology and welfare. Thus, we examined how an incremental temperature increase alone (Warm & Normoxic-WN: 12→20°C; 1°C week -1 ), and in combination with moderate hypoxia (Warm & Hypoxic-WH: ~70% air saturation), impacted salmon’s hepatic transcriptome expression compared to control fish (CT: 12°C, normoxic) using 44K microarrays and qPCR. Results: Overall, we identified 2,894 differentially expressed probes (DEPs, FDR < 5%), that included 1,111 shared DEPs, while 789 and 994 DEPs were specific to WN and WH fish, respectively. Pathway analysis suggested that the cellular mechanisms affected by the two experimental conditions were quite similar, with up-regulated genes functionally associated with heat shock response, ER-stress, apoptosis and immune defence, while genes connected with general metabolic processes, proteolysis and oxidation-reduction were largely suppressed. The qPCR assessment of 41 microarray-identified genes validated that the heat shock response ( hsp90aa1, serpinh1 ), apoptosis ( casp8, jund, jak2 ) and immune responses ( apod, c1ql2, epx ) were up-regulated in WN and WH fish, while oxidative stress and hypoxia sensitive genes were down-regulated ( cirbp, cyp1a1, egln2, gstt1, hif1α, prdx6, rraga, ucp2 ). However, the additional challenge of hypoxia resulted in more pronounced effects on heat shock and immune-related processes, including a stronger influence on the expression of 14 immune-related genes. Finally, robust correlations between the transcription of 19 genes and several phenotypic traits in WH fish suggest that changes in gene expression were related to an impaired physiological and growth performance. Conclusion: Increasing temperature to 20°C alone, and in combination with hypoxia, resulted in the up- and down-regulation of genes involved in similar important pathways in Atlantic salmon. However, the heat shock and immune responses of fish exposed to 20°C and hypoxia were more affected, and their transcriptional dysregulation was related to reduced performance. This study provides valuable information on how these two environmental challenges affect the expression of stress-, metabolic- and immune-related genes and pathways and identifies potential biomarker genes for improving our understanding of fish health and welfare." @default.
- W4245129849 created "2022-05-12" @default.
- W4245129849 creator A5005183831 @default.
- W4245129849 creator A5020184311 @default.
- W4245129849 creator A5026539778 @default.
- W4245129849 creator A5068713579 @default.
- W4245129849 creator A5074199427 @default.
- W4245129849 creator A5075546378 @default.
- W4245129849 date "2020-07-28" @default.
- W4245129849 modified "2023-10-16" @default.
- W4245129849 title "The Transcriptomic Responses of Atlantic Salmon (Salmo salar) to High Temperature Stress Alone, and in Combination with Moderate Hypoxia" @default.
- W4245129849 doi "https://doi.org/10.21203/rs.3.rs-38228/v1" @default.
- W4245129849 hasPublicationYear "2020" @default.
- W4245129849 type Work @default.
- W4245129849 citedByCount "0" @default.
- W4245129849 crossrefType "posted-content" @default.
- W4245129849 hasAuthorship W4245129849A5005183831 @default.
- W4245129849 hasAuthorship W4245129849A5020184311 @default.
- W4245129849 hasAuthorship W4245129849A5026539778 @default.
- W4245129849 hasAuthorship W4245129849A5068713579 @default.
- W4245129849 hasAuthorship W4245129849A5074199427 @default.
- W4245129849 hasAuthorship W4245129849A5075546378 @default.
- W4245129849 hasBestOaLocation W42451298491 @default.
- W4245129849 hasConcept C104317684 @default.
- W4245129849 hasConcept C137984847 @default.
- W4245129849 hasConcept C150194340 @default.
- W4245129849 hasConcept C153911025 @default.
- W4245129849 hasConcept C162317418 @default.
- W4245129849 hasConcept C16685009 @default.
- W4245129849 hasConcept C178790620 @default.
- W4245129849 hasConcept C185592680 @default.
- W4245129849 hasConcept C186836561 @default.
- W4245129849 hasConcept C203014093 @default.
- W4245129849 hasConcept C205260736 @default.
- W4245129849 hasConcept C2776151105 @default.
- W4245129849 hasConcept C2777940460 @default.
- W4245129849 hasConcept C2779018089 @default.
- W4245129849 hasConcept C2909208804 @default.
- W4245129849 hasConcept C505870484 @default.
- W4245129849 hasConcept C540031477 @default.
- W4245129849 hasConcept C55493867 @default.
- W4245129849 hasConcept C68991219 @default.
- W4245129849 hasConcept C71924100 @default.
- W4245129849 hasConcept C7836513 @default.
- W4245129849 hasConcept C8415881 @default.
- W4245129849 hasConcept C86803240 @default.
- W4245129849 hasConcept C8891405 @default.
- W4245129849 hasConcept C95444343 @default.
- W4245129849 hasConceptScore W4245129849C104317684 @default.
- W4245129849 hasConceptScore W4245129849C137984847 @default.
- W4245129849 hasConceptScore W4245129849C150194340 @default.
- W4245129849 hasConceptScore W4245129849C153911025 @default.
- W4245129849 hasConceptScore W4245129849C162317418 @default.
- W4245129849 hasConceptScore W4245129849C16685009 @default.
- W4245129849 hasConceptScore W4245129849C178790620 @default.
- W4245129849 hasConceptScore W4245129849C185592680 @default.
- W4245129849 hasConceptScore W4245129849C186836561 @default.
- W4245129849 hasConceptScore W4245129849C203014093 @default.
- W4245129849 hasConceptScore W4245129849C205260736 @default.
- W4245129849 hasConceptScore W4245129849C2776151105 @default.
- W4245129849 hasConceptScore W4245129849C2777940460 @default.
- W4245129849 hasConceptScore W4245129849C2779018089 @default.
- W4245129849 hasConceptScore W4245129849C2909208804 @default.
- W4245129849 hasConceptScore W4245129849C505870484 @default.
- W4245129849 hasConceptScore W4245129849C540031477 @default.
- W4245129849 hasConceptScore W4245129849C55493867 @default.
- W4245129849 hasConceptScore W4245129849C68991219 @default.
- W4245129849 hasConceptScore W4245129849C71924100 @default.
- W4245129849 hasConceptScore W4245129849C7836513 @default.
- W4245129849 hasConceptScore W4245129849C8415881 @default.
- W4245129849 hasConceptScore W4245129849C86803240 @default.
- W4245129849 hasConceptScore W4245129849C8891405 @default.
- W4245129849 hasConceptScore W4245129849C95444343 @default.
- W4245129849 hasLocation W42451298491 @default.
- W4245129849 hasLocation W42451298492 @default.
- W4245129849 hasLocation W42451298493 @default.
- W4245129849 hasOpenAccess W4245129849 @default.
- W4245129849 hasPrimaryLocation W42451298491 @default.
- W4245129849 hasRelatedWork W2002357097 @default.
- W4245129849 hasRelatedWork W2003434208 @default.
- W4245129849 hasRelatedWork W2013135109 @default.
- W4245129849 hasRelatedWork W2048437533 @default.
- W4245129849 hasRelatedWork W2070600272 @default.
- W4245129849 hasRelatedWork W2734403094 @default.
- W4245129849 hasRelatedWork W2890995448 @default.
- W4245129849 hasRelatedWork W3121049759 @default.
- W4245129849 hasRelatedWork W3169420937 @default.
- W4245129849 hasRelatedWork W4245129849 @default.
- W4245129849 isParatext "false" @default.
- W4245129849 isRetracted "false" @default.
- W4245129849 workType "article" @default.