Matches in SemOpenAlex for { <https://semopenalex.org/work/W4245431390> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W4245431390 endingPage "1571" @default.
- W4245431390 startingPage "1564" @default.
- W4245431390 abstract "We perfused livers from fed rats with a balanced salt solution containing 1 mmol/L glucose. Under these conditions a low steady rate of glycogenolysis was observed (approximately 1.7 μmol glucose equivalents/g/min; 20% of the maximal glycogenolytic activity). Nitric oxide (NO) transiently stimulated hepatic glucose production. A maximal response (on average doubling basal glucose output) was observed with 34 μmol/L NO. The same concentration of nitrite (NO2-) was ineffective. Half-maximal effects were seen at 8 to 10 μmol/L NO, irrespective of the flow direction (portocaval or retrograde). This glycogenolytic response to NO corresponded to a partial activation of phosphorylase. The NO effect was not additive to maximal stimulation of glycogenolysis (7.7 ± 0.2 μmol hexose equivalents/g/min; n = 4) by 100 μmol/L dibutyryl cyclic adenosine monophosphate (Bt2cAMP). The requirement for activation of phosphorylase was also evidenced by the ineffectiveness of NO in phosphorylase-kinase-deficient livers of gsd/gsd rats. The NO effect was blocked by co-administration of cyclooxygenase inhibitors (50 μmol/L ibuprofen, 50 μmol/L indomethacin, or 2 mmol/L aspirin), suggesting a mediatory role of prostanoids from nonparenchymal cells. This conclusion was confirmed by the fact that NO did not activate phosphorylase in isolated hepatocytes. Moreover, NO was no longer glycogenolytic in livers perfused with Ca2+-free medium, in agreement with the known mediatory role of Ca2+ in prostanoid-mediated responses. Surprisingly, in Ca2+- free medium NO inhibited the basal glucose production. This coincided with an increased elution of cyclic guanosine monophosphate (cGMP). Inhibition of glycogenolysis by NO under these conditions was blocked by 1 mmol/L theophylline, suggestive for involvement of cGMP-stimulated cAMP phosphodiesterase. However, we could not confirm that an increase in cGMP resulted in a drop in cAMP. In conclusion, NO recruits opposing mechanisms with respect to modulation of basal hepatic glycogenolysis. In the presence of Ca2+, activation of phosphorylase with stimulation of glycogenolysis dominates. Cyclooxygenase inhibitors abolish this effect. Activation by NO of the cyclooxygenase in nonparenchymal cells is a distinct possibility. In the absence of Ca2+, inhibition of basal glycogenolysis becomes observable. It remains to be established whether this results from cGMP-mediated stimulation of hydrolysis of cAMP." @default.
- W4245431390 created "2022-05-12" @default.
- W4245431390 creator A5049402764 @default.
- W4245431390 date "1996-06-01" @default.
- W4245431390 modified "2023-10-17" @default.
- W4245431390 title "Modulation of basal hepatic glycogenolysis by nitric oxide" @default.
- W4245431390 doi "https://doi.org/10.1053/jhep.1996.v23.pm0008675178" @default.
- W4245431390 hasPublicationYear "1996" @default.
- W4245431390 type Work @default.
- W4245431390 citedByCount "0" @default.
- W4245431390 crossrefType "journal-article" @default.
- W4245431390 hasAuthorship W4245431390A5049402764 @default.
- W4245431390 hasBestOaLocation W42454313901 @default.
- W4245431390 hasConcept C126322002 @default.
- W4245431390 hasConcept C134018914 @default.
- W4245431390 hasConcept C181199279 @default.
- W4245431390 hasConcept C185592680 @default.
- W4245431390 hasConcept C204779464 @default.
- W4245431390 hasConcept C24998067 @default.
- W4245431390 hasConcept C2776991684 @default.
- W4245431390 hasConcept C2777499176 @default.
- W4245431390 hasConcept C2777949327 @default.
- W4245431390 hasConcept C2777995097 @default.
- W4245431390 hasConcept C2778024521 @default.
- W4245431390 hasConcept C2779306644 @default.
- W4245431390 hasConcept C2779689624 @default.
- W4245431390 hasConcept C2780994212 @default.
- W4245431390 hasConcept C519581460 @default.
- W4245431390 hasConcept C55493867 @default.
- W4245431390 hasConcept C62231903 @default.
- W4245431390 hasConcept C71924100 @default.
- W4245431390 hasConcept C86803240 @default.
- W4245431390 hasConceptScore W4245431390C126322002 @default.
- W4245431390 hasConceptScore W4245431390C134018914 @default.
- W4245431390 hasConceptScore W4245431390C181199279 @default.
- W4245431390 hasConceptScore W4245431390C185592680 @default.
- W4245431390 hasConceptScore W4245431390C204779464 @default.
- W4245431390 hasConceptScore W4245431390C24998067 @default.
- W4245431390 hasConceptScore W4245431390C2776991684 @default.
- W4245431390 hasConceptScore W4245431390C2777499176 @default.
- W4245431390 hasConceptScore W4245431390C2777949327 @default.
- W4245431390 hasConceptScore W4245431390C2777995097 @default.
- W4245431390 hasConceptScore W4245431390C2778024521 @default.
- W4245431390 hasConceptScore W4245431390C2779306644 @default.
- W4245431390 hasConceptScore W4245431390C2779689624 @default.
- W4245431390 hasConceptScore W4245431390C2780994212 @default.
- W4245431390 hasConceptScore W4245431390C519581460 @default.
- W4245431390 hasConceptScore W4245431390C55493867 @default.
- W4245431390 hasConceptScore W4245431390C62231903 @default.
- W4245431390 hasConceptScore W4245431390C71924100 @default.
- W4245431390 hasConceptScore W4245431390C86803240 @default.
- W4245431390 hasIssue "6" @default.
- W4245431390 hasLocation W42454313901 @default.
- W4245431390 hasOpenAccess W4245431390 @default.
- W4245431390 hasPrimaryLocation W42454313901 @default.
- W4245431390 hasRelatedWork W2026433816 @default.
- W4245431390 hasRelatedWork W2033761402 @default.
- W4245431390 hasRelatedWork W2056528285 @default.
- W4245431390 hasRelatedWork W2063041624 @default.
- W4245431390 hasRelatedWork W2064311032 @default.
- W4245431390 hasRelatedWork W2101457200 @default.
- W4245431390 hasRelatedWork W2410373927 @default.
- W4245431390 hasRelatedWork W2905993336 @default.
- W4245431390 hasRelatedWork W4245431390 @default.
- W4245431390 hasRelatedWork W2480560083 @default.
- W4245431390 hasVolume "23" @default.
- W4245431390 isParatext "false" @default.
- W4245431390 isRetracted "false" @default.
- W4245431390 workType "article" @default.