Matches in SemOpenAlex for { <https://semopenalex.org/work/W4245697989> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W4245697989 abstract "Abstract Background: Isocitrate dehydrogenase (IDH1/2) gene mutations are the most frequently observed mutations in cartilaginous tumors. The mutant IDH causes elevation in the levels of R-enantiomer of 2-hydroxylglutarate (R-2HG). Mesenchymal stromal cells (MSCs) are reasonable precursor cell candidates of cartilaginous tumors. This study aimed to investigate the effect of oncometabolite R-2HG on MSCs. Methods: Human bone marrow MSCs treated with or without R-2HG at concentrations 0.1 to 1.5mM were used for experiments. Cell Counting Kit-8 was used to detect the proliferation of MSCs. To determine the effects of R-2HG on MSC differentiation, cells were cultured in osteogenic, chondrogenic and adipogenic medium. Specific staining approaches were performed and differentiation-related genes were quantified. Furthermore, DNA methylation status was explored by Illumina array-based arrays. Real-time PCR was applied to examine the signaling component mRNAs involved in. Results: R-2HG showed no influence on the proliferation of human MSCs. R-2HG blocked osteogenic differentiation, whereas promoted adipogenic differentiation of MSCs in a dose-dependent manner. R-2HG inhibited chondrogenic differentiation of MSCs, but increased the expression of genes related to chondrocyte hypertrophy in a lower concentration (1.0 mM). Moreover, R-2HG induced a pronounced DNA hypermethylation state of MSC. R-2HG also improved promotor methylation of lineage-specific genes during osteogenic and chondrogenic differentiation. In addition, R-2HG induced hypermethylation and decreased the mRNA levels of SHH, GLI1and GLI2, indicating Sonic Hedgehog (Shh) signaling inhibition. Conclusions: The oncometabolite R-2HG dysregulated the chondrogenic and osteogenic differentiation of MSCs possibly via induction of DNA hypermethylation, improving the role of R-2HG in cartilaginous tumor development." @default.
- W4245697989 created "2022-05-12" @default.
- W4245697989 creator A5010632162 @default.
- W4245697989 creator A5026662451 @default.
- W4245697989 creator A5033443215 @default.
- W4245697989 creator A5034191619 @default.
- W4245697989 creator A5054111036 @default.
- W4245697989 creator A5067050151 @default.
- W4245697989 creator A5068244825 @default.
- W4245697989 creator A5068580768 @default.
- W4245697989 creator A5076257659 @default.
- W4245697989 creator A5090013547 @default.
- W4245697989 date "2021-01-10" @default.
- W4245697989 modified "2023-09-27" @default.
- W4245697989 title "The oncometabolite R-2-hydroxyglutarate dysregulates the differentiation of human mesenchymal stromal cells via inducing DNA hypermethylation" @default.
- W4245697989 doi "https://doi.org/10.21203/rs.3.rs-18260/v3" @default.
- W4245697989 hasPublicationYear "2021" @default.
- W4245697989 type Work @default.
- W4245697989 citedByCount "0" @default.
- W4245697989 crossrefType "posted-content" @default.
- W4245697989 hasAuthorship W4245697989A5010632162 @default.
- W4245697989 hasAuthorship W4245697989A5026662451 @default.
- W4245697989 hasAuthorship W4245697989A5033443215 @default.
- W4245697989 hasAuthorship W4245697989A5034191619 @default.
- W4245697989 hasAuthorship W4245697989A5054111036 @default.
- W4245697989 hasAuthorship W4245697989A5067050151 @default.
- W4245697989 hasAuthorship W4245697989A5068244825 @default.
- W4245697989 hasAuthorship W4245697989A5068580768 @default.
- W4245697989 hasAuthorship W4245697989A5076257659 @default.
- W4245697989 hasAuthorship W4245697989A5090013547 @default.
- W4245697989 hasBestOaLocation W42456979891 @default.
- W4245697989 hasConcept C104317684 @default.
- W4245697989 hasConcept C122927707 @default.
- W4245697989 hasConcept C148738053 @default.
- W4245697989 hasConcept C150194340 @default.
- W4245697989 hasConcept C153911025 @default.
- W4245697989 hasConcept C185592680 @default.
- W4245697989 hasConcept C190727270 @default.
- W4245697989 hasConcept C198826908 @default.
- W4245697989 hasConcept C2778486617 @default.
- W4245697989 hasConcept C35496256 @default.
- W4245697989 hasConcept C502942594 @default.
- W4245697989 hasConcept C55493867 @default.
- W4245697989 hasConcept C86803240 @default.
- W4245697989 hasConcept C95444343 @default.
- W4245697989 hasConceptScore W4245697989C104317684 @default.
- W4245697989 hasConceptScore W4245697989C122927707 @default.
- W4245697989 hasConceptScore W4245697989C148738053 @default.
- W4245697989 hasConceptScore W4245697989C150194340 @default.
- W4245697989 hasConceptScore W4245697989C153911025 @default.
- W4245697989 hasConceptScore W4245697989C185592680 @default.
- W4245697989 hasConceptScore W4245697989C190727270 @default.
- W4245697989 hasConceptScore W4245697989C198826908 @default.
- W4245697989 hasConceptScore W4245697989C2778486617 @default.
- W4245697989 hasConceptScore W4245697989C35496256 @default.
- W4245697989 hasConceptScore W4245697989C502942594 @default.
- W4245697989 hasConceptScore W4245697989C55493867 @default.
- W4245697989 hasConceptScore W4245697989C86803240 @default.
- W4245697989 hasConceptScore W4245697989C95444343 @default.
- W4245697989 hasLocation W42456979891 @default.
- W4245697989 hasLocation W42456979892 @default.
- W4245697989 hasLocation W42456979893 @default.
- W4245697989 hasOpenAccess W4245697989 @default.
- W4245697989 hasPrimaryLocation W42456979891 @default.
- W4245697989 hasRelatedWork W1977744140 @default.
- W4245697989 hasRelatedWork W2000228770 @default.
- W4245697989 hasRelatedWork W2087691169 @default.
- W4245697989 hasRelatedWork W2912091564 @default.
- W4245697989 hasRelatedWork W2915780375 @default.
- W4245697989 hasRelatedWork W3012664368 @default.
- W4245697989 hasRelatedWork W3164287290 @default.
- W4245697989 hasRelatedWork W3201468063 @default.
- W4245697989 hasRelatedWork W4221055048 @default.
- W4245697989 hasRelatedWork W4296402822 @default.
- W4245697989 isParatext "false" @default.
- W4245697989 isRetracted "false" @default.
- W4245697989 workType "article" @default.