FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4245705226> ?p ?o ?g. }

• W4245705226 endingPage "326" @default.
• W4245705226 startingPage "319" @default.
• W4245705226 abstract "Perfusion of medium through three-dimensional (3D) collagen sponges enhanced viability and function of cocultivated marrow stromal and hematopoietic cell lines. Cells of the murine bone marrow stromal cell line GPIa were cultured in novel 3D collagen sponges, made from pepsin-digested bovine skin. Static cultures of sponges were maintained in dishes with media changes every other day. Perfused sponges were contained in a glass column with medium flow set at 1.3 mL/min. In some sponges, the 32D cl3 c-fms(m) (CRX-1) hematopoietic progenitor cell line was added 7 days after GPIa cells. At 7 and 16 days, light microscopic evaluation showed poor viability of cells in static sponge cultures. In perfused sponge cultures, there was greater cellularity throughout the sponge and abundant accumulation of metachromatic extracellular matrix surrounding GPIa cells. Chondroitin 6-sulfate and heparan sulfate were identified as components of the matrix by immunohistochemical methods. DNA synthesis was evaluated by 15-h exposure of cultures to bromodeoxyuridine (BrdU), with subsequent immunohistochemical localization with monoclonal anti-BrdU antibody. Cells positive for BrdU were identified at the outer surfaces of both static and perfused sponges; however, positive cells were also seen throughout the internal areas of the sponges that were perfused. These results suggest that better nutrient exchange occurred in perfused sponges. In static cocultures of GPIa and CRX-1 cells, there was no detectable viability of the IL-3-dependent CRX-1 cells; however, under perfused conditions, CRX-1 cells flourished within the sponges as documented by BrdU incorporation. Thus, medium perfusion enhanced GPIa stromal cell line viability and function in 3D collagen sponge cultures, as demonstrated by BrdU incorporation, matrix production, and support of CRX-1 cells. This novel culture system may be useful for examining the interactions of bone marrow stromal cells with extracellular matrix molecules, soluble and matrix-bound factors, and with other cell types." @default.
• W4245705226 created "2022-05-12" @default.
• W4245705226 creator A5041981667 @default.
• W4245705226 creator A5063266916 @default.
• W4245705226 creator A5070880955 @default.
• W4245705226 date "1998-05-01" @default.
• W4245705226 modified "2023-10-18" @default.
• W4245705226 title "Perfusion Enhances Functions of Bone Marrow Stromal Cells in Three-Dimensional Culture" @default.
• W4245705226 cites W1265257573 @default.
• W4245705226 cites W1970202636 @default.
• W4245705226 cites W1977387775 @default.
• W4245705226 cites W1979415894 @default.
• W4245705226 cites W1983773059 @default.
• W4245705226 cites W1988420415 @default.
• W4245705226 cites W1990934385 @default.
• W4245705226 cites W1996403236 @default.
• W4245705226 cites W1998347190 @default.
• W4245705226 cites W2003275504 @default.
• W4245705226 cites W2004807762 @default.
• W4245705226 cites W2039045865 @default.
• W4245705226 cites W2041222342 @default.
• W4245705226 cites W2053018628 @default.
• W4245705226 cites W2059067233 @default.
• W4245705226 cites W2086886868 @default.
• W4245705226 cites W2095582203 @default.
• W4245705226 cites W2115898073 @default.
• W4245705226 cites W2117713457 @default.
• W4245705226 cites W2125489880 @default.
• W4245705226 cites W2132063386 @default.
• W4245705226 cites W2267993214 @default.
• W4245705226 cites W2321057541 @default.
• W4245705226 cites W2333597718 @default.
• W4245705226 cites W2371130252 @default.
• W4245705226 doi "https://doi.org/10.1177/096368979800700310" @default.
• W4245705226 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9647441" @default.
• W4245705226 hasPublicationYear "1998" @default.
• W4245705226 type Work @default.
• W4245705226 citedByCount "58" @default.
• W4245705226 countsByYear W42457052262014 @default.
• W4245705226 countsByYear W42457052262015 @default.
• W4245705226 countsByYear W42457052262016 @default.
• W4245705226 countsByYear W42457052262017 @default.
• W4245705226 countsByYear W42457052262018 @default.
• W4245705226 countsByYear W42457052262019 @default.
• W4245705226 countsByYear W42457052262020 @default.
• W4245705226 crossrefType "journal-article" @default.
• W4245705226 hasAuthorship W4245705226A5041981667 @default.
• W4245705226 hasAuthorship W4245705226A5063266916 @default.
• W4245705226 hasAuthorship W4245705226A5070880955 @default.
• W4245705226 hasConcept C106487976 @default.
• W4245705226 hasConcept C1491633281 @default.
• W4245705226 hasConcept C153911025 @default.
• W4245705226 hasConcept C16930146 @default.
• W4245705226 hasConcept C185592680 @default.
• W4245705226 hasConcept C189165786 @default.
• W4245705226 hasConcept C203014093 @default.
• W4245705226 hasConcept C2775895372 @default.
• W4245705226 hasConcept C2778260397 @default.
• W4245705226 hasConcept C2778849931 @default.
• W4245705226 hasConcept C2779335624 @default.
• W4245705226 hasConcept C2780007613 @default.
• W4245705226 hasConcept C43617362 @default.
• W4245705226 hasConcept C502942594 @default.
• W4245705226 hasConcept C53227056 @default.
• W4245705226 hasConcept C54355233 @default.
• W4245705226 hasConcept C55493867 @default.
• W4245705226 hasConcept C59822182 @default.
• W4245705226 hasConcept C62112901 @default.
• W4245705226 hasConcept C81885089 @default.
• W4245705226 hasConcept C86803240 @default.
• W4245705226 hasConcept C95444343 @default.
• W4245705226 hasConceptScore W4245705226C106487976 @default.
• W4245705226 hasConceptScore W4245705226C1491633281 @default.
• W4245705226 hasConceptScore W4245705226C153911025 @default.
• W4245705226 hasConceptScore W4245705226C16930146 @default.
• W4245705226 hasConceptScore W4245705226C185592680 @default.
• W4245705226 hasConceptScore W4245705226C189165786 @default.
• W4245705226 hasConceptScore W4245705226C203014093 @default.
• W4245705226 hasConceptScore W4245705226C2775895372 @default.
• W4245705226 hasConceptScore W4245705226C2778260397 @default.
• W4245705226 hasConceptScore W4245705226C2778849931 @default.
• W4245705226 hasConceptScore W4245705226C2779335624 @default.
• W4245705226 hasConceptScore W4245705226C2780007613 @default.
• W4245705226 hasConceptScore W4245705226C43617362 @default.
• W4245705226 hasConceptScore W4245705226C502942594 @default.
• W4245705226 hasConceptScore W4245705226C53227056 @default.
• W4245705226 hasConceptScore W4245705226C54355233 @default.
• W4245705226 hasConceptScore W4245705226C55493867 @default.
• W4245705226 hasConceptScore W4245705226C59822182 @default.
• W4245705226 hasConceptScore W4245705226C62112901 @default.
• W4245705226 hasConceptScore W4245705226C81885089 @default.
• W4245705226 hasConceptScore W4245705226C86803240 @default.
• W4245705226 hasConceptScore W4245705226C95444343 @default.
• W4245705226 hasIssue "3" @default.
• W4245705226 hasLocation W42457052261 @default.
• W4245705226 hasLocation W42457052262 @default.
• W4245705226 hasOpenAccess W4245705226 @default.
• W4245705226 hasPrimaryLocation W42457052261 @default.

• next