Matches in SemOpenAlex for { <https://semopenalex.org/work/W4245785763> ?p ?o ?g. }
- W4245785763 endingPage "2225" @default.
- W4245785763 startingPage "2222" @default.
- W4245785763 abstract "Abstract The selective generation of covalent bonds between and within proteins would provide new avenues for studying protein function and engineering proteins with new properties. New covalent bonds were genetically introduced into proteins by enabling an unnatural amino acid (Uaa) to selectively react with a proximal natural residue. This proximity‐enabled bioreactivity was expanded to a series of haloalkane Uaas. Orthogonal tRNA/synthetase pairs were evolved to incorporate these Uaas, which only form a covalent thioether bond with cysteine when positioned in close proximity. By using the Uaa and cysteine, spontaneous covalent bond formation was demonstrated between an affibody and its substrate Z protein, thereby leading to irreversible binding, and within the affibody to increase its thermostability. This strategy of proximity‐enabled protein crosslinking (PEPC) may be generally expanded to target different natural amino acids, thus providing diversity and flexibility in covalent bond formation for protein research and protein engineering." @default.
- W4245785763 created "2022-05-12" @default.
- W4245785763 creator A5011497989 @default.
- W4245785763 creator A5046221512 @default.
- W4245785763 creator A5053680151 @default.
- W4245785763 creator A5055005369 @default.
- W4245785763 creator A5065097720 @default.
- W4245785763 creator A5069137038 @default.
- W4245785763 creator A5073216396 @default.
- W4245785763 date "2014-01-21" @default.
- W4245785763 modified "2023-10-08" @default.
- W4245785763 title "Proximity‐Enabled Protein Crosslinking through Genetically Encoding Haloalkane Unnatural Amino Acids" @default.
- W4245785763 cites W1994822882 @default.
- W4245785763 cites W2011489155 @default.
- W4245785763 cites W2018400595 @default.
- W4245785763 cites W2045950957 @default.
- W4245785763 cites W2050957868 @default.
- W4245785763 cites W2051031878 @default.
- W4245785763 cites W2061377697 @default.
- W4245785763 cites W2072594799 @default.
- W4245785763 cites W2127699503 @default.
- W4245785763 cites W2134178604 @default.
- W4245785763 cites W2135235247 @default.
- W4245785763 cites W2136639243 @default.
- W4245785763 cites W2146913917 @default.
- W4245785763 cites W2148313902 @default.
- W4245785763 cites W2149554987 @default.
- W4245785763 cites W2157680389 @default.
- W4245785763 cites W2800284038 @default.
- W4245785763 doi "https://doi.org/10.1002/ange.201308794" @default.
- W4245785763 hasPublicationYear "2014" @default.
- W4245785763 type Work @default.
- W4245785763 citedByCount "16" @default.
- W4245785763 countsByYear W42457857632014 @default.
- W4245785763 countsByYear W42457857632016 @default.
- W4245785763 countsByYear W42457857632017 @default.
- W4245785763 countsByYear W42457857632018 @default.
- W4245785763 countsByYear W42457857632019 @default.
- W4245785763 countsByYear W42457857632022 @default.
- W4245785763 crossrefType "journal-article" @default.
- W4245785763 hasAuthorship W4245785763A5011497989 @default.
- W4245785763 hasAuthorship W4245785763A5046221512 @default.
- W4245785763 hasAuthorship W4245785763A5053680151 @default.
- W4245785763 hasAuthorship W4245785763A5055005369 @default.
- W4245785763 hasAuthorship W4245785763A5065097720 @default.
- W4245785763 hasAuthorship W4245785763A5069137038 @default.
- W4245785763 hasAuthorship W4245785763A5073216396 @default.
- W4245785763 hasBestOaLocation W42457857632 @default.
- W4245785763 hasConcept C104317684 @default.
- W4245785763 hasConcept C147816474 @default.
- W4245785763 hasConcept C152769699 @default.
- W4245785763 hasConcept C153957851 @default.
- W4245785763 hasConcept C178790620 @default.
- W4245785763 hasConcept C180577832 @default.
- W4245785763 hasConcept C181199279 @default.
- W4245785763 hasConcept C185592680 @default.
- W4245785763 hasConcept C2779201268 @default.
- W4245785763 hasConcept C2779315393 @default.
- W4245785763 hasConcept C2781216036 @default.
- W4245785763 hasConcept C47701112 @default.
- W4245785763 hasConcept C515207424 @default.
- W4245785763 hasConcept C55493867 @default.
- W4245785763 hasConcept C67705224 @default.
- W4245785763 hasConcept C71240020 @default.
- W4245785763 hasConceptScore W4245785763C104317684 @default.
- W4245785763 hasConceptScore W4245785763C147816474 @default.
- W4245785763 hasConceptScore W4245785763C152769699 @default.
- W4245785763 hasConceptScore W4245785763C153957851 @default.
- W4245785763 hasConceptScore W4245785763C178790620 @default.
- W4245785763 hasConceptScore W4245785763C180577832 @default.
- W4245785763 hasConceptScore W4245785763C181199279 @default.
- W4245785763 hasConceptScore W4245785763C185592680 @default.
- W4245785763 hasConceptScore W4245785763C2779201268 @default.
- W4245785763 hasConceptScore W4245785763C2779315393 @default.
- W4245785763 hasConceptScore W4245785763C2781216036 @default.
- W4245785763 hasConceptScore W4245785763C47701112 @default.
- W4245785763 hasConceptScore W4245785763C515207424 @default.
- W4245785763 hasConceptScore W4245785763C55493867 @default.
- W4245785763 hasConceptScore W4245785763C67705224 @default.
- W4245785763 hasConceptScore W4245785763C71240020 @default.
- W4245785763 hasIssue "8" @default.
- W4245785763 hasLocation W42457857631 @default.
- W4245785763 hasLocation W42457857632 @default.
- W4245785763 hasOpenAccess W4245785763 @default.
- W4245785763 hasPrimaryLocation W42457857631 @default.
- W4245785763 hasRelatedWork W1973834553 @default.
- W4245785763 hasRelatedWork W2031196168 @default.
- W4245785763 hasRelatedWork W2042181696 @default.
- W4245785763 hasRelatedWork W2045388299 @default.
- W4245785763 hasRelatedWork W2057422942 @default.
- W4245785763 hasRelatedWork W2079077885 @default.
- W4245785763 hasRelatedWork W3020850360 @default.
- W4245785763 hasRelatedWork W3215076145 @default.
- W4245785763 hasRelatedWork W4284965515 @default.
- W4245785763 hasRelatedWork W4318051960 @default.
- W4245785763 hasVolume "126" @default.
- W4245785763 isParatext "false" @default.
- W4245785763 isRetracted "false" @default.