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- W4245919793 abstract "A naphthalene (Nap) and salicylate (Sal) degrading microorganism, Pseudomonas putida RKJ1, is chemotactic towards these compounds. This strain carries a 83 kb plasmid. A 25 kb EcoRI fragment of the plasmid contains the genes responsible for Nap degradation through Sal. RKJ5, the plasmid-cured derivative of RKJ1, is neither capable of degradation nor is chemotactic towards Nap or Sal. The recombinant plasmid pRKJ3, which contained a 25 kb EcoRI fragment, was transferred back into the plasmid-free wild-type strain RKJ5, and the transconjugant showed both degradation and chemotaxis. The recombinant plasmid pRKJ3 was also transferred into motile, plasmid-free P. putida KT2442. The resulting transconjugant (RKJ15) showed chemotaxis towards both Nap and Sal. Two mutant strains carrying deletions in pRKJ3 (in KT2442) with phenotypes Nap - Sal + and Nap - Sal - , were also tested for chemotaxis. It was found that the Nap - Sal + mutant strain showed chemotaxis towards Sal only, whereas the Nap - Sal - mutant strain is non-chemotactic towards both the compounds. These results suggest that the metabolism of Nap and Sal may be required for the chemotactic activity.Key words: Pseudomonas putida, plasmid-encoded chemotaxis, naphthalene, salicylate." @default.
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- W4245919793 date "1999-12-17" @default.
- W4245919793 modified "2023-10-16" @default.
- W4245919793 title "Evidence for plasmid-mediated chemotaxis ofPseudomonas putidatowards naphthalene and salicylate" @default.
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- W4245919793 doi "https://doi.org/10.1139/w99-118" @default.
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