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- W4246031663 abstract "OP-1 is a main member of the BMP family. It plays diverse and significant roles in growth and differentiation. RT-PCR amplified the open reading frame of mouse OP-1 from cDNAs synthesis from CD-1 mouse embryo. By coding the gene of mOP-1 was inserted into pTrcHis 2B, the prokaryotic expression vector through IPTG induction, and the recombinant protein was isolated and purified with Ni-NTA resin. In an ALP activity assay, rmOP-1 has shown osteogenesis activity in vitro. It would offer a fast and inexpensive means of the productions of recombinant protein and an easy tool for growth and development research on rmOP-1." @default.
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- W4246031663 date "2002-03-01" @default.
- W4246031663 modified "2023-09-27" @default.
- W4246031663 title "PROKARYOTIC EXPRESSION OF RECOMBINANT MOUSE OP-1 AND ITS OSTEOGENESISIN VITRO" @default.
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- W4246031663 doi "https://doi.org/10.1142/s0218957702000769" @default.
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