FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4246223895> ?p ?o ?g. }

• W4246223895 endingPage "3228" @default.
• W4246223895 startingPage "3217" @default.
• W4246223895 abstract "GCN4 is a transcriptional activator of amino acid biosynthetic genes in Saccharomyces cerevisiae whose expression is regulated by amino-acid availability at the translational level. GCD1 and GCD2 are negative regulators required for the repression of GCN4 translation under nonstarvation conditions that is mediated by upstream open reading frames (uORFs) in the leader of GCN4 mRNA. GCD factors are thought to be antagonized by the positive regulators GCN1, GCN2 and GCN3 in amino acid-starved cells to allow for increased GCN4 protein synthesis. Previous genetic studies suggested that GCD1, GCD2, and GCN3 have closely related functions in the regulation of GCN4 expression that involve translation initiation factor 2 (eIF-2). In agreement with these predictions, we show that GCD1, GCD2, and GCN3 are integral components of a high-molecular-weight complex of approximately 600,000 Da. The three proteins copurified through several biochemical fractionation steps and could be coimmunoprecipitated by using antibodies against GCD1 or GCD2. Interestingly, a portion of the eIF-2 present in cell extracts also cofractionated and coimmunoprecipitated with these regulatory proteins but was dissociated from the GCD1/GCD2/GCN3 complex by 0.5 M KCl. Incubation of a temperature-sensitive gcdl-101 mutant at the restrictive temperature led to a rapid reduction in the average size and quantity of polysomes, plus an accumulation of inactive 80S ribosomal couples; in addition, excess amounts of eIF-2 alpha, GCD1, GCD2, and GCN3 were found comigrating with free 40S ribosomal subunits. These results suggest that GCD1 is required for an essential function involving eIF-2 at a late step in the translation initiation cycle. We propose that lowering the function of this high-molecular-weight complex, or of eIF-2 itself, in amino acid-starved cells leads to reduced ribosomal recognition of the uORFs and increased translation initiation at the GCN4 start codon. Our results provide new insights into how general initiation factors can be regulated to affect gene-specific translational control." @default.
• W4246223895 created "2022-05-12" @default.
• W4246223895 creator A5015593109 @default.
• W4246223895 creator A5035404530 @default.
• W4246223895 creator A5090025131 @default.
• W4246223895 creator A5091088864 @default.
• W4246223895 date "1991-06-01" @default.
• W4246223895 modified "2023-10-16" @default.
• W4246223895 title "Complex Formation by Positive and Negative Translational Regulators of <i>GCN4</i>" @default.
• W4246223895 cites W1481139836 @default.
• W4246223895 cites W1483444054 @default.
• W4246223895 cites W1491237609 @default.
• W4246223895 cites W1503789453 @default.
• W4246223895 cites W1511858736 @default.
• W4246223895 cites W1513838543 @default.
• W4246223895 cites W1569310536 @default.
• W4246223895 cites W1573100887 @default.
• W4246223895 cites W1582070860 @default.
• W4246223895 cites W1589355384 @default.
• W4246223895 cites W1593443523 @default.
• W4246223895 cites W1601827412 @default.
• W4246223895 cites W1662971183 @default.
• W4246223895 cites W1769175751 @default.
• W4246223895 cites W1853827546 @default.
• W4246223895 cites W1886701353 @default.
• W4246223895 cites W1896726814 @default.
• W4246223895 cites W1910618147 @default.
• W4246223895 cites W1965177762 @default.
• W4246223895 cites W1969107860 @default.
• W4246223895 cites W1969737313 @default.
• W4246223895 cites W1973782344 @default.
• W4246223895 cites W1978186743 @default.
• W4246223895 cites W1979202944 @default.
• W4246223895 cites W1979412818 @default.
• W4246223895 cites W1988134822 @default.
• W4246223895 cites W1999019973 @default.
• W4246223895 cites W2014423141 @default.
• W4246223895 cites W2018432619 @default.
• W4246223895 cites W2022016707 @default.
• W4246223895 cites W2037724550 @default.
• W4246223895 cites W2047134288 @default.
• W4246223895 cites W2047662834 @default.
• W4246223895 cites W2049690336 @default.
• W4246223895 cites W2053955340 @default.
• W4246223895 cites W2066825851 @default.
• W4246223895 cites W2100837269 @default.
• W4246223895 cites W2101108802 @default.
• W4246223895 cites W2103832597 @default.
• W4246223895 cites W2119958357 @default.
• W4246223895 cites W2137350042 @default.
• W4246223895 cites W2141606719 @default.
• W4246223895 cites W2144879264 @default.
• W4246223895 cites W2169465564 @default.
• W4246223895 cites W2224929038 @default.
• W4246223895 cites W2493996218 @default.
• W4246223895 cites W4234615114 @default.
• W4246223895 cites W4241000956 @default.
• W4246223895 cites W4244965997 @default.
• W4246223895 cites W4249769297 @default.
• W4246223895 cites W4255797102 @default.
• W4246223895 cites W4293247451 @default.
• W4246223895 cites W72118176 @default.
• W4246223895 doi "https://doi.org/10.1128/mcb.11.6.3217-3228.1991" @default.
• W4246223895 hasPublicationYear "1991" @default.
• W4246223895 type Work @default.
• W4246223895 citedByCount "6" @default.
• W4246223895 crossrefType "journal-article" @default.
• W4246223895 hasAuthorship W4246223895A5015593109 @default.
• W4246223895 hasAuthorship W4246223895A5035404530 @default.
• W4246223895 hasAuthorship W4246223895A5090025131 @default.
• W4246223895 hasAuthorship W4246223895A5091088864 @default.
• W4246223895 hasConcept C100631289 @default.
• W4246223895 hasConcept C12554922 @default.
• W4246223895 hasConcept C181199279 @default.
• W4246223895 hasConcept C54355233 @default.
• W4246223895 hasConcept C55493867 @default.
• W4246223895 hasConcept C70721500 @default.
• W4246223895 hasConcept C86803240 @default.
• W4246223895 hasConcept C95444343 @default.
• W4246223895 hasConceptScore W4246223895C100631289 @default.
• W4246223895 hasConceptScore W4246223895C12554922 @default.
• W4246223895 hasConceptScore W4246223895C181199279 @default.
• W4246223895 hasConceptScore W4246223895C54355233 @default.
• W4246223895 hasConceptScore W4246223895C55493867 @default.
• W4246223895 hasConceptScore W4246223895C70721500 @default.
• W4246223895 hasConceptScore W4246223895C86803240 @default.
• W4246223895 hasConceptScore W4246223895C95444343 @default.
• W4246223895 hasIssue "6" @default.
• W4246223895 hasLocation W42462238951 @default.
• W4246223895 hasOpenAccess W4246223895 @default.
• W4246223895 hasPrimaryLocation W42462238951 @default.
• W4246223895 hasRelatedWork W1828691184 @default.
• W4246223895 hasRelatedWork W1903732681 @default.
• W4246223895 hasRelatedWork W1991523530 @default.
• W4246223895 hasRelatedWork W2002128513 @default.
• W4246223895 hasRelatedWork W2020824267 @default.
• W4246223895 hasRelatedWork W2031436818 @default.
• W4246223895 hasRelatedWork W2057739827 @default.

• next