Matches in SemOpenAlex for { <https://semopenalex.org/work/W4246304467> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W4246304467 endingPage "845" @default.
- W4246304467 startingPage "839" @default.
- W4246304467 abstract "The occurrence of two enzymes performing de‐N‐glycosylation of glycoproteins, namely, endo‐N‐acetyl‐β‐ d‐glucosaminidase (ENGase, EC 3.2.1.96) and peptide‐N4‐(N‐acetyl‐β‐d‐glucosaminyl) asparagine amidase (PNGase, EC 3.5.1.52) was investigated in barley, cv. Plaisant (a winter six rowed variety). The dry grain showed both activities according to the HPLC detection of the hydrolysis of fluorescent resorufin‐labelled substrates. However, PNGase activity was 16‐fold higher than ENGase activity. During germination, both activities increased, PNGase by only 1.5‐fold compared to nearly 4.8‐fold for ENGase over the 4 d following imbibition. The localization of these activities within the grain showed that the major contribution of PNGase was due to the endosperm, typically representing over 90% of the whole grain activity. In contrast, ENGase activity was especially high in the embryo and, later, in the developing plantlet (10‐fold higher than in the endosperm), particularly in the rootlets and scutellum. In developing spikes, PNGase activity was 5.6‐fold higher than in the leaves, but similar ENGase activity was measured in both organs. During grain formation, PNGase activity followed dry matter increase together with endosperm development. In contrast, ENGase activity dropped by 66% at the beginning of grain filling before stabilizing until harvest. The occurrence of de‐N‐glycosylation‐performing enzymes throughout the development of barley raises the question of the nature of their natural substrates. Moreover, the prevalence of one of these enzymes over the other depending on the organ and the developmental stage, could represent the first evidence of specific functions for each enzyme." @default.
- W4246304467 created "2022-05-12" @default.
- W4246304467 creator A5014802995 @default.
- W4246304467 creator A5021340251 @default.
- W4246304467 creator A5038366773 @default.
- W4246304467 creator A5052372115 @default.
- W4246304467 creator A5066746667 @default.
- W4246304467 date "2000-05-01" @default.
- W4246304467 modified "2023-09-30" @default.
- W4246304467 title "Evidence of two enzymes performing the de‐N‐glycosylation of proteins in barley: expression during germination, localization within the grain and set‐up during grain formation" @default.
- W4246304467 cites W1606498175 @default.
- W4246304467 cites W1968334347 @default.
- W4246304467 cites W1972931582 @default.
- W4246304467 cites W1990132059 @default.
- W4246304467 cites W1996949997 @default.
- W4246304467 cites W2015088136 @default.
- W4246304467 cites W2020363463 @default.
- W4246304467 cites W2023451072 @default.
- W4246304467 cites W2024208316 @default.
- W4246304467 cites W2048530018 @default.
- W4246304467 cites W2064433652 @default.
- W4246304467 cites W2067397663 @default.
- W4246304467 cites W2079437832 @default.
- W4246304467 cites W2094802855 @default.
- W4246304467 cites W2138357365 @default.
- W4246304467 cites W2897045455 @default.
- W4246304467 cites W2990325914 @default.
- W4246304467 cites W4245792343 @default.
- W4246304467 cites W4296322132 @default.
- W4246304467 doi "https://doi.org/10.1093/jxb/51.346.839" @default.
- W4246304467 hasPublicationYear "2000" @default.
- W4246304467 type Work @default.
- W4246304467 citedByCount "0" @default.
- W4246304467 crossrefType "journal-article" @default.
- W4246304467 hasAuthorship W4246304467A5014802995 @default.
- W4246304467 hasAuthorship W4246304467A5021340251 @default.
- W4246304467 hasAuthorship W4246304467A5038366773 @default.
- W4246304467 hasAuthorship W4246304467A5052372115 @default.
- W4246304467 hasAuthorship W4246304467A5066746667 @default.
- W4246304467 hasBestOaLocation W42463044671 @default.
- W4246304467 hasConcept C100701293 @default.
- W4246304467 hasConcept C100836998 @default.
- W4246304467 hasConcept C108625454 @default.
- W4246304467 hasConcept C113442657 @default.
- W4246304467 hasConcept C181199279 @default.
- W4246304467 hasConcept C185592680 @default.
- W4246304467 hasConcept C206212055 @default.
- W4246304467 hasConcept C2775895851 @default.
- W4246304467 hasConcept C2776817266 @default.
- W4246304467 hasConcept C2777313579 @default.
- W4246304467 hasConcept C2778409621 @default.
- W4246304467 hasConcept C2993665447 @default.
- W4246304467 hasConcept C46757340 @default.
- W4246304467 hasConcept C55493867 @default.
- W4246304467 hasConcept C59822182 @default.
- W4246304467 hasConcept C86803240 @default.
- W4246304467 hasConceptScore W4246304467C100701293 @default.
- W4246304467 hasConceptScore W4246304467C100836998 @default.
- W4246304467 hasConceptScore W4246304467C108625454 @default.
- W4246304467 hasConceptScore W4246304467C113442657 @default.
- W4246304467 hasConceptScore W4246304467C181199279 @default.
- W4246304467 hasConceptScore W4246304467C185592680 @default.
- W4246304467 hasConceptScore W4246304467C206212055 @default.
- W4246304467 hasConceptScore W4246304467C2775895851 @default.
- W4246304467 hasConceptScore W4246304467C2776817266 @default.
- W4246304467 hasConceptScore W4246304467C2777313579 @default.
- W4246304467 hasConceptScore W4246304467C2778409621 @default.
- W4246304467 hasConceptScore W4246304467C2993665447 @default.
- W4246304467 hasConceptScore W4246304467C46757340 @default.
- W4246304467 hasConceptScore W4246304467C55493867 @default.
- W4246304467 hasConceptScore W4246304467C59822182 @default.
- W4246304467 hasConceptScore W4246304467C86803240 @default.
- W4246304467 hasIssue "346" @default.
- W4246304467 hasLocation W42463044671 @default.
- W4246304467 hasOpenAccess W4246304467 @default.
- W4246304467 hasPrimaryLocation W42463044671 @default.
- W4246304467 hasRelatedWork W161448031 @default.
- W4246304467 hasRelatedWork W1976118749 @default.
- W4246304467 hasRelatedWork W1979046329 @default.
- W4246304467 hasRelatedWork W1984939224 @default.
- W4246304467 hasRelatedWork W2018128719 @default.
- W4246304467 hasRelatedWork W2019751362 @default.
- W4246304467 hasRelatedWork W2054205538 @default.
- W4246304467 hasRelatedWork W2936098593 @default.
- W4246304467 hasRelatedWork W63663339 @default.
- W4246304467 hasRelatedWork W68544838 @default.
- W4246304467 hasVolume "51" @default.
- W4246304467 isParatext "false" @default.
- W4246304467 isRetracted "false" @default.
- W4246304467 workType "article" @default.