Matches in SemOpenAlex for { <https://semopenalex.org/work/W4246371361> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W4246371361 endingPage "S270" @default.
- W4246371361 startingPage "S269" @default.
- W4246371361 abstract "1823 Chronic pharmacologic anabolic-androgenic steroid (AAS) treatment using testosterone propionate (TP) has previously been shown to improve the force generating capabilities of the diaphragm muscle of gonadally intact adult male rats without altering cross-sectional area. PURPOSE: The purpose of this study is to determine the effect of AAS treatment on satellite cell activation in the diaphragm muscle. METHODS: Adult male rats (250–275 g initial body weight) were housed in the USC vivarium with food and water supplied ad libium. Serum testosterone levels were elevated to approximately 10x physiologic levels for up to 28 days through the use of subcutaneously implanted Silastic™ capsules filled with crystalline TP. Animals were deeply anesthetized and killed by transcardial saline perfusion followed by 4% paraformaldehyde in situ. The right and left costal diaphragm muscle (DIAm) were harvested and prepared for immunohistochemistry. Immunohistochemical analysis was performed on 10 μm-thick sections utilizing standard protocols. Goat anti-Mcadherin, Santa Cruz, CA (satellite cells), rabbit anti-Laminin, DAKO, Denmark (basal lamina), rabbit anti-MyoD, Santa Cruz, CA, (myogenic regulatory factor) were used to visualize to identify quiescent and activated satellite cells. Nuclei were labeled with DAPI (Molecular Probes, OR). One-way ANOVA with Bonferroni correction was used to determine differences between groups. RESULTS: Significant increases in DIAm quiescent satellite cell number, relative to controls, were observed after 7-days of TP treatment, (39.3 and 16.0, respectively; F = 4.97, p = 0.01). Interestingly, quiescent DIAm satellite cell numbers appear to return to control values after 14 and 28 days of TP treatment. Cross-sectional area (μm2) and number of myonuclei per fiber were not affected by TP treatment (F = 0.45, p = NS, and F = 0.59, p = NS, respectively). CONCLUSIONS: These data suggest that pharmacologic TP treatment transiently increases quiescent satellite cell number. These observations suggest that TP treatment of sedentary adult male rats transiently increases the capability of this muscle to rapidly adapt to physiologic stresses by increasing the number of cells capable of myogenic differentiation. (Research funded by NHLBI: HL-59904 to CEB)." @default.
- W4246371361 created "2022-05-12" @default.
- W4246371361 creator A5019371023 @default.
- W4246371361 creator A5052661603 @default.
- W4246371361 creator A5074326978 @default.
- W4246371361 creator A5085094402 @default.
- W4246371361 date "2004-05-01" @default.
- W4246371361 modified "2023-10-18" @default.
- W4246371361 title "Pharmacologic Androgen Treatment Induces Satellite Cell Activity in the Rat Diaphragm" @default.
- W4246371361 doi "https://doi.org/10.1249/00005768-200405001-01294" @default.
- W4246371361 hasPublicationYear "2004" @default.
- W4246371361 type Work @default.
- W4246371361 citedByCount "0" @default.
- W4246371361 crossrefType "journal-article" @default.
- W4246371361 hasAuthorship W4246371361A5019371023 @default.
- W4246371361 hasAuthorship W4246371361A5052661603 @default.
- W4246371361 hasAuthorship W4246371361A5074326978 @default.
- W4246371361 hasAuthorship W4246371361A5085094402 @default.
- W4246371361 hasConcept C105702510 @default.
- W4246371361 hasConcept C126322002 @default.
- W4246371361 hasConcept C134018914 @default.
- W4246371361 hasConcept C16685009 @default.
- W4246371361 hasConcept C178790620 @default.
- W4246371361 hasConcept C185592680 @default.
- W4246371361 hasConcept C204232928 @default.
- W4246371361 hasConcept C207200792 @default.
- W4246371361 hasConcept C2777911890 @default.
- W4246371361 hasConcept C2778106681 @default.
- W4246371361 hasConcept C2778723968 @default.
- W4246371361 hasConcept C2779279991 @default.
- W4246371361 hasConcept C2781115071 @default.
- W4246371361 hasConcept C3763915 @default.
- W4246371361 hasConcept C71315377 @default.
- W4246371361 hasConcept C71924100 @default.
- W4246371361 hasConcept C86803240 @default.
- W4246371361 hasConceptScore W4246371361C105702510 @default.
- W4246371361 hasConceptScore W4246371361C126322002 @default.
- W4246371361 hasConceptScore W4246371361C134018914 @default.
- W4246371361 hasConceptScore W4246371361C16685009 @default.
- W4246371361 hasConceptScore W4246371361C178790620 @default.
- W4246371361 hasConceptScore W4246371361C185592680 @default.
- W4246371361 hasConceptScore W4246371361C204232928 @default.
- W4246371361 hasConceptScore W4246371361C207200792 @default.
- W4246371361 hasConceptScore W4246371361C2777911890 @default.
- W4246371361 hasConceptScore W4246371361C2778106681 @default.
- W4246371361 hasConceptScore W4246371361C2778723968 @default.
- W4246371361 hasConceptScore W4246371361C2779279991 @default.
- W4246371361 hasConceptScore W4246371361C2781115071 @default.
- W4246371361 hasConceptScore W4246371361C3763915 @default.
- W4246371361 hasConceptScore W4246371361C71315377 @default.
- W4246371361 hasConceptScore W4246371361C71924100 @default.
- W4246371361 hasConceptScore W4246371361C86803240 @default.
- W4246371361 hasIssue "Supplement" @default.
- W4246371361 hasLocation W42463713611 @default.
- W4246371361 hasOpenAccess W4246371361 @default.
- W4246371361 hasPrimaryLocation W42463713611 @default.
- W4246371361 hasRelatedWork W1977970588 @default.
- W4246371361 hasRelatedWork W1992438146 @default.
- W4246371361 hasRelatedWork W2011296913 @default.
- W4246371361 hasRelatedWork W2014764273 @default.
- W4246371361 hasRelatedWork W2022659954 @default.
- W4246371361 hasRelatedWork W2023266651 @default.
- W4246371361 hasRelatedWork W2101146068 @default.
- W4246371361 hasRelatedWork W2116930034 @default.
- W4246371361 hasRelatedWork W2146554192 @default.
- W4246371361 hasRelatedWork W2611776224 @default.
- W4246371361 hasVolume "36" @default.
- W4246371361 isParatext "false" @default.
- W4246371361 isRetracted "false" @default.
- W4246371361 workType "article" @default.