Matches in SemOpenAlex for { <https://semopenalex.org/work/W4246372182> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W4246372182 endingPage "50" @default.
- W4246372182 startingPage "45" @default.
- W4246372182 abstract "The acrA strain AS-1 of Escherichia coli is more sensitive than its parent W3110 to growth inhibition by Methylene blue sodium dodecyl sulfate and novobiocin. UR-3 is an uncoupler-resistant strain isolated from AS-1 which is resistant to growth inhibition by carbonylcyanide m-chlorophenylhydrazone (CCCP). 3.3′.4.5-tetrachlorsalicylanilide (TCS) and tributyltin chloride, while remaining sensitive to the first group of compounds. A revertant of AS-1 acquired resistance to Methylene blue and sodium dodecyl sulfate but emained sensitive to uncouplers. In contrast to AS-1, proline uptake in UR-3 was resistant to uncouplers. Strain UR-3 grown in the presence of uncoupler incorporated elongation factor Tu to high levels in the outer membrane of the cell. A role for the outer membrane in the acquisition of uncoupler-resistance by UR-3 is suggested by the behaviour of the mutant to the fluorescene probe N-phenyl-1-naphthylamine. The fluorescence intensity of this probe was quenched by membrane energization in the wild-type strain W3110 but not in AS-1. UR-3 behaved like W3110, suggesting that an outer membrane barrier to neutral lipophilic compounds like N-phenyl-1-naphthylamine (NPN) and uncoupler had been restored in UR-3. By contrast, AS-1 and UR-3 both allowed energized uptake of the fluorescent lipophilic cation 2-(dimethylaminostyryl)-1-ethylpyridinium (DMP). It is concluded that lipophilic materials must permeate the outer membrane of E. coli by at least two different routes. However, uncoupler-resistance in UR-3 appears to be more complex than the provision of an outer membrane barrier to uncouplers. Thus, uncouplers readily discharged a pH gradient established in both AS-1 and UR-3 by addition of HCl to cell suspensions." @default.
- W4246372182 created "2022-05-12" @default.
- W4246372182 creator A5061046330 @default.
- W4246372182 date "1992-01-30" @default.
- W4246372182 modified "2023-09-25" @default.
- W4246372182 title "Differential permeability for lipophilic compounds in uncoupler-resistant cells of Escherichia coli" @default.
- W4246372182 doi "https://doi.org/10.1016/0167-4838(92)90450-r" @default.
- W4246372182 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1739727" @default.
- W4246372182 hasPublicationYear "1992" @default.
- W4246372182 type Work @default.
- W4246372182 citedByCount "1" @default.
- W4246372182 crossrefType "journal-article" @default.
- W4246372182 hasAuthorship W4246372182A5061046330 @default.
- W4246372182 hasConcept C104317684 @default.
- W4246372182 hasConcept C105702510 @default.
- W4246372182 hasConcept C12554922 @default.
- W4246372182 hasConcept C146587185 @default.
- W4246372182 hasConcept C161790260 @default.
- W4246372182 hasConcept C178790620 @default.
- W4246372182 hasConcept C181911157 @default.
- W4246372182 hasConcept C185592680 @default.
- W4246372182 hasConcept C2778022156 @default.
- W4246372182 hasConcept C2778913249 @default.
- W4246372182 hasConcept C2779191827 @default.
- W4246372182 hasConcept C2992270614 @default.
- W4246372182 hasConcept C41625074 @default.
- W4246372182 hasConcept C43617362 @default.
- W4246372182 hasConcept C537181965 @default.
- W4246372182 hasConcept C547475151 @default.
- W4246372182 hasConcept C55493867 @default.
- W4246372182 hasConcept C65165184 @default.
- W4246372182 hasConcept C86803240 @default.
- W4246372182 hasConceptScore W4246372182C104317684 @default.
- W4246372182 hasConceptScore W4246372182C105702510 @default.
- W4246372182 hasConceptScore W4246372182C12554922 @default.
- W4246372182 hasConceptScore W4246372182C146587185 @default.
- W4246372182 hasConceptScore W4246372182C161790260 @default.
- W4246372182 hasConceptScore W4246372182C178790620 @default.
- W4246372182 hasConceptScore W4246372182C181911157 @default.
- W4246372182 hasConceptScore W4246372182C185592680 @default.
- W4246372182 hasConceptScore W4246372182C2778022156 @default.
- W4246372182 hasConceptScore W4246372182C2778913249 @default.
- W4246372182 hasConceptScore W4246372182C2779191827 @default.
- W4246372182 hasConceptScore W4246372182C2992270614 @default.
- W4246372182 hasConceptScore W4246372182C41625074 @default.
- W4246372182 hasConceptScore W4246372182C43617362 @default.
- W4246372182 hasConceptScore W4246372182C537181965 @default.
- W4246372182 hasConceptScore W4246372182C547475151 @default.
- W4246372182 hasConceptScore W4246372182C55493867 @default.
- W4246372182 hasConceptScore W4246372182C65165184 @default.
- W4246372182 hasConceptScore W4246372182C86803240 @default.
- W4246372182 hasIssue "1" @default.
- W4246372182 hasLocation W42463721821 @default.
- W4246372182 hasLocation W42463721822 @default.
- W4246372182 hasOpenAccess W4246372182 @default.
- W4246372182 hasPrimaryLocation W42463721821 @default.
- W4246372182 hasRelatedWork W1267014805 @default.
- W4246372182 hasRelatedWork W2003357470 @default.
- W4246372182 hasRelatedWork W2022669366 @default.
- W4246372182 hasRelatedWork W2064080902 @default.
- W4246372182 hasRelatedWork W2071951393 @default.
- W4246372182 hasRelatedWork W2081943401 @default.
- W4246372182 hasRelatedWork W2105157144 @default.
- W4246372182 hasRelatedWork W2121160741 @default.
- W4246372182 hasRelatedWork W2150792357 @default.
- W4246372182 hasRelatedWork W2270501460 @default.
- W4246372182 hasVolume "1099" @default.
- W4246372182 isParatext "false" @default.
- W4246372182 isRetracted "false" @default.
- W4246372182 workType "article" @default.