Matches in SemOpenAlex for { <https://semopenalex.org/work/W4246790717> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W4246790717 abstract "<p>Ion mobility was integrated with liquid chromatography/high resolution mass spectrometry (LC/IM-HRMS) to quantify 25-hydroxyvitamin D (25OHD) in human serum. It has previously been shown that 25OHD adopts two gas-phase conformations which are resolved using ion mobility; in contrast, the inactive epimer, 3-epi-25-hydroxyvitamin D (epi25OHD) only adopts one. Interference from epi25OHD was eliminated by filtering the chromatogram to retain the drift time that corresponds to the unique gas-phase conformation of 25OHD. Although ion mobility separates the epimers, some chromatography is required to separate compounds which interfere with ionization or fall at the same nominal <i>m/z</i>. Standards were prepared in 4% albumin solutions and compared against commercial serum quality controls. Standards and quality controls were analyzed and validated using a two-minute LC/IM-MS method. 25-hydroxyvitamin D3 and D2 were quantified over the range between 2 and 500 ng/mL with bias and precision within 15%. When epi25OHD was spiked into quality control samples, no significant bias was introduced, and analysis of 30 patient samples shows good agreement between this LC/IM-MS and traditional LC/MS/MS methods. This work shows that ion mobility can be incorporated with liquid chromatography and mass spectrometry for rapid quantitation of 25OHD in human serum.</p>" @default.
- W4246790717 created "2022-05-12" @default.
- W4246790717 creator A5010665082 @default.
- W4246790717 creator A5011252927 @default.
- W4246790717 creator A5021570473 @default.
- W4246790717 creator A5041147061 @default.
- W4246790717 creator A5048042532 @default.
- W4246790717 creator A5069619631 @default.
- W4246790717 creator A5088003120 @default.
- W4246790717 date "2019-06-11" @default.
- W4246790717 modified "2023-09-26" @default.
- W4246790717 title "Rapid Quantitation of 25-Hydroxyvitamin D2 and D3 in Human Serum Using Liquid Chromatography/drift Tube Ion Mobility-Mass Spectrometry" @default.
- W4246790717 doi "https://doi.org/10.26434/chemrxiv.8247176" @default.
- W4246790717 hasPublicationYear "2019" @default.
- W4246790717 type Work @default.
- W4246790717 citedByCount "0" @default.
- W4246790717 crossrefType "posted-content" @default.
- W4246790717 hasAuthorship W4246790717A5010665082 @default.
- W4246790717 hasAuthorship W4246790717A5011252927 @default.
- W4246790717 hasAuthorship W4246790717A5021570473 @default.
- W4246790717 hasAuthorship W4246790717A5041147061 @default.
- W4246790717 hasAuthorship W4246790717A5048042532 @default.
- W4246790717 hasAuthorship W4246790717A5069619631 @default.
- W4246790717 hasAuthorship W4246790717A5088003120 @default.
- W4246790717 hasConcept C113196181 @default.
- W4246790717 hasConcept C138268822 @default.
- W4246790717 hasConcept C145148216 @default.
- W4246790717 hasConcept C15213710 @default.
- W4246790717 hasConcept C154945302 @default.
- W4246790717 hasConcept C162356407 @default.
- W4246790717 hasConcept C175467004 @default.
- W4246790717 hasConcept C178790620 @default.
- W4246790717 hasConcept C185592680 @default.
- W4246790717 hasConcept C198291218 @default.
- W4246790717 hasConcept C203130289 @default.
- W4246790717 hasConcept C41008148 @default.
- W4246790717 hasConcept C43617362 @default.
- W4246790717 hasConcept C64489805 @default.
- W4246790717 hasConceptScore W4246790717C113196181 @default.
- W4246790717 hasConceptScore W4246790717C138268822 @default.
- W4246790717 hasConceptScore W4246790717C145148216 @default.
- W4246790717 hasConceptScore W4246790717C15213710 @default.
- W4246790717 hasConceptScore W4246790717C154945302 @default.
- W4246790717 hasConceptScore W4246790717C162356407 @default.
- W4246790717 hasConceptScore W4246790717C175467004 @default.
- W4246790717 hasConceptScore W4246790717C178790620 @default.
- W4246790717 hasConceptScore W4246790717C185592680 @default.
- W4246790717 hasConceptScore W4246790717C198291218 @default.
- W4246790717 hasConceptScore W4246790717C203130289 @default.
- W4246790717 hasConceptScore W4246790717C41008148 @default.
- W4246790717 hasConceptScore W4246790717C43617362 @default.
- W4246790717 hasConceptScore W4246790717C64489805 @default.
- W4246790717 hasLocation W42467907171 @default.
- W4246790717 hasOpenAccess W4246790717 @default.
- W4246790717 hasPrimaryLocation W42467907171 @default.
- W4246790717 hasRelatedWork W10846234 @default.
- W4246790717 hasRelatedWork W21003272 @default.
- W4246790717 hasRelatedWork W2243583 @default.
- W4246790717 hasRelatedWork W2339581 @default.
- W4246790717 hasRelatedWork W28056001 @default.
- W4246790717 hasRelatedWork W32718931 @default.
- W4246790717 hasRelatedWork W33377625 @default.
- W4246790717 hasRelatedWork W33794738 @default.
- W4246790717 hasRelatedWork W33986596 @default.
- W4246790717 hasRelatedWork W3075982 @default.
- W4246790717 isParatext "false" @default.
- W4246790717 isRetracted "false" @default.
- W4246790717 workType "article" @default.