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- W4246850867 abstract "In the presence of phenethylalcohol, plasmidencoded levansucrase (2,6β-d-fructan: 6,β-d-fructosyltransferase, EC 2.4.1.10) was accumulated as a precursor form in the membrane fraction of Escherichia coli minicells. Immunoblotting experiments were used to evaluate the amount of the newly synthesised precursor form relative to the immunoreactivity of the purified mature form of levansucrase. Solubilisation of both forms with Triton X100 and Triton X114 was compared. Triton X114 allowed separation of the precursor form from the mature form, which were recovered in the detergent and the aqueous phases, respectively, after separation of the phases at 30°C." @default.
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- W4246850867 date "1985-11-01" @default.
- W4246850867 modified "2023-09-30" @default.
- W4246850867 title "Preferential synthesis, localisation and solubilisation of a precursor form of Bacillus subtilis levansucrase in an Escherichia coli minicell system" @default.
- W4246850867 doi "https://doi.org/10.1016/0378-1097(85)90383-0" @default.
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